Abstract
The ultimate goal of regenerative therapy is to develop fully functioning bioengineered organs that can replace lost or damaged organs after disease, injury, or aging. We have previously developed a three-dimensional culture system with the aim of reconstituting a bioengineered organ germ at an early developmental stage. The regeneration of a functional tooth unit is critical issue to achieving proper oral function, including mastication. Recently, we successfully demonstrated that our bioengineered tooth germ could develop a fully functioning tooth with sufficient hardness for masticatory potential, the ability to withstand mechanical stress in the maxillofacial region, and in which the innerved neural fibers had an adequate perceptive potential for noxious stimulations. Our results thus show that bioengineered tooth germ can develop a fully functioning regenerated tooth in vivo after engraftment and therefore that organ replacement regenerative therapy in this way is feasible.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Brockes JP, Kumar A (2005) Appendage regeneration in adult vertebrates and implications for regenerative medicine. Science 310:1919–1923
Watt FM, Hogan BL (2000) Out of Eden: stem cells and their niches. Science 287:1427–1430
Langer RS, Vacanti JP (1999) Tissue engineering: the challenges ahead. Sci Am 280:86–89
Atala A (2005) Tissue engineering, stem cells and cloning: current concepts and changing trends. Expert Opin Biol Ther 5:879–892
Korbling M, Estrov Z (2003) Adult stem cells for tissue repair – a new therapeutic concept? N Engl J Med 349:570–582
Griffith LG, Naughton G (2002) Tissue engineering – current challenges and expanding opportunities. Science 295:1009–1014
Ikeda E, Tsuji T (2008) Growing bioengineered teeth from single cells: potential for dental regenerative medicine. Expert Opin Biol Ther 8:735–744
Purnell B (2008) New release: the complete guide to organ repair. Introduction. Science 322:1489
Lechler RI, Sykes M, Thomson AW et al (2005) Organ transplantation – how much of the promise has been realized? Nat Med 11:605–613
Sharpe PT, Young CS (2005) Test-tube teeth. Sci Am 293:34–41
Duailibi SE, Duailibi MT, Vacanti JP et al (2006) Prospects for tooth regeneration. Periodontol 2000 41:177–187
Voruganti K (2008) Clinical periodontology and implant dentistry, 5th edition. Br Dent J 205:216
Tsukiboshi M (1993) Autogenous tooth transplantation: a reevaluation. Int J Periodontics Restorative Dent 13(2):120–149
Pispa J, Thesleff I (2003) Mechanisms of ectodermal organogenesis. Dev Biol 262:195–205
Tucker A, Sharpe P (2004) The cutting-edge of mammalian development; how the embryo makes teeth. Nat Rev Genet 5:499–508
Nakao K, Morita R, Saji Y et al (2007) The development of a bioengineered organ germ method. Nat Methods 4:227–230
Ikeda E, Morita R, Nakao K et al (2009) Fully functional bioengineered tooth replacement as an organ replacement therapy. Proc Natl Acad Sci U S A 106:13475–13480
Manly RS, Braley LC (1950) Masticatory performance and efficiency. J Dent Res 29:448–462
Shimono M, Ishikawa T, Ishikawa H et al (2003) Regulatory mechanisms of periodontal regeneration. Microsc Res Tech 60:491–502
Byers MR, Narhi MV (1999) Dental injury models: experimental tools for understanding neuroinflammatory interactions and polymodal nociceptor functions. Crit Rev Oral Biol Med 10:4–39
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Springer
About this paper
Cite this paper
Nakao, K., Tsuji, T. (2010). Strategies underlying research in tooth regenerative therapy as a possible model for future organ replacement. In: Sasano, T., Suzuki, O. (eds) Interface Oral Health Science 2009. Springer, Tokyo. https://doi.org/10.1007/978-4-431-99644-6_3
Download citation
DOI: https://doi.org/10.1007/978-4-431-99644-6_3
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-99643-9
Online ISBN: 978-4-431-99644-6
eBook Packages: MedicineMedicine (R0)