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In-vivo Optical Imaging of Cortical Architecture and Dynamics

  • Chapter
Modern Techniques in Neuroscience Research

Abstract

A number of new imaging techniques are available to scientists to visualize the functioning brain directly, revealing unprecedented details. These imaging techniques have provided a new level of understanding of the principles underlying cortical development, organization and function. In this chapter we will focus on optical imaging in the living mammalian brain, using two complementary imaging techniques. The first technique is based on intrinsic signals. The second technique is based on voltage-sensitive dyes. Currently, these two optical imaging techniques offer the best spatial and temporal resolution, but also have inherent limitations. We shall provide a few examples of new findings obtained mostly in work done in our laboratory. The focus will be upon the understanding of methodological aspects which in turn should contribute to optimal use of these imaging techniques. General reviews describing earlier work done on simpler preparations have been published elsewhere (Cohen, 1973; Tasaki and Warashina, 1976; Waggoner and Grinvald, 1977; Waggoner, 1979; Salzberg, 1983; Grinvald, 1984; Grinvald et al., 1985; De Weer and Salzberg, 1986; Cohen and Lesher, 1986; Salzberg et al., 1986; Loew, 1987; Orbach, 1987; Blasdel, 1988, 1989; Grinvald et al., 1988; Kamino, 1991; Cinelli and Kauer, 1992; Frostig, 1994).

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  1. Amiram Grinvald
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  2. D. Shoham
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  8. C. Wijnbergen
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Editors and Affiliations

  1. Center of Musculoskeletat Research, National Institute for Working Life, Petrus Laestadius väg, 90713, Umeå, Sweden

    Uwe Windhorst  & Håkan Johansson  & 

  2. Department of Clinical Neurosciences and Department of Physiology and Biophysics Faculty of Medicine, The University of Calgary, 333 Hospital Drive N.W., Calgary, Alberta, T2N 4N1, Canada

    Uwe Windhorst

  3. Zentrum Physiologie und Pathophysiologie, Universität Göttingen, Humboldtallee 23, 37073, Göttingen, Germany

    Uwe Windhorst

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Grinvald, A. et al. (1999). In-vivo Optical Imaging of Cortical Architecture and Dynamics. In: Windhorst, U., Johansson, H. (eds) Modern Techniques in Neuroscience Research. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-58552-4_34

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