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Laboratory diagnostics

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Hepatology Textbook and Atlas

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Abstract

In the two volumes of the classical textbook of hepatology by F. Th. Frerichs “Klinik der Leberkrankheiten” (“Clinical aspects of liver diseases”; 1858, 1861), there is no mention of laboratory diagnostics. (s. figs. 1.19, 1.20) • According to our knowledge, laboratory diagnostics was first presented in 1923 by G. Lepehne in his book “Die Leberfunktionsprüfungen, ihre Ergebnisse und ihre Methodik” (“Liver function tests, their results and methods”). This monograph initiated the 1 st epoch of biochemical liver diagnostics. The laboratory field was again addressed by H. Eppinger in 1937 in his textbook “Die Leberkrankheiten” (“Liver diseases”) consisting of 52 pages; enzyme tests, however, were not mentioned.(s. fig. 5.1)

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References Correct laboratory diagnostics

  1. Fiedler, G.M., Thiery, J.: The incorrect laboratory result. Part 1: Pre-and post analytical phase. Internist 2004; 43: 315–332

    Article  Google Scholar 

  2. Harm, K., Dieckvoss, E., Nagel, H.-H., Voigt, K.D.: Alters-und Geschlechtsabhängigkeit klinisch-chemischer Kenngrößen. Lab. Med. 1981; 5: 134–141

    Google Scholar 

  3. Junge, B., Hoffmeister, H., Feddersen, H.-M., Röcker, L.: Standardisierung der Blutentnahme. Einfluß der Stauung auf 33 Blut-und Serumbestandteile Dtsch. Med. Wschr. 1978; 103: 260–265

    PubMed  CAS  Google Scholar 

  4. Keller, H., Staber, G.: Arzneimittel-Interferenzen bei klinisch-chemischen Tests. Lab. Med 1983; 7: 164–171

    Google Scholar 

  5. Massarat, S., Herbert, V.: Haltbarkeit der Serumenzyme beim Versand. Dtsch. Ärztebl. 1977; 74: 1909–1910

    Google Scholar 

  6. Sonntag, O.: Haemolysis as an interference factor in clinical chemistry. J. Clin. Chem. Clin. Biochem. 1986; 24: 127–139

    PubMed  CAS  Google Scholar 

  7. Thomas, L.: Blutentnahme und Probentransport. Dtsch. Ärztebl. 1991; 88: 1873–1877

    Google Scholar 

  8. Wisser, H., Knoll, E.: Tageszeitliche Änderungen klinisch-chemischer Meßgrößen. Ärztl. Labor 1982; 28: 99–108

    Google Scholar 

Selection criteria for liver tests

  1. Blum, H.E., Weizsäcker, von, F.: Rationelle klinisch-chemische Diagnostik hepatobiliärer Erkrankungen. Internist 1991; 32: 239–243

    PubMed  CAS  Google Scholar 

  2. Chopra, S., Griffin, P.H.: Laboratory tests and diagnostic procedures in evaluation of liver disease. Amer. J. Med. 1985; 79: 221–230

    Article  PubMed  CAS  Google Scholar 

  3. Irrgang, B., Adam, N., Pätzold, K., Przybylski, F.: Zur Testauswahl für ein labordiagnostisches Stufenprogramm zur gezielten Unterstützung der Diagnostik von Lebererkrankungen. Z. Klin. Med. 1990; 45: 2223–2227

    Google Scholar 

  4. Kuntz, E.: Aktuelle laborchemische Leberfunktionsdiagnostik. Münch. Med. Wschr. 1972; 114: 781–788, 830–835

    PubMed  CAS  Google Scholar 

  5. Kuntz, E.: Rationelle Diagnostik der Leberkrankheiten. Fortschr. Med. 1987; 105: 297–299, 335–336, 377–378, 419–420, 433–434, 454–456, 552, 593–594, 641–642, 660–662

    Google Scholar 

  6. Popper, H., Medline, A.: Die “Organellen-Pathologie”. Ihre Bedeutung bei der Beurteilung von Leberfunktionsproben. Münch. Med. Wschr. 1969; 111: 1569–1574

    PubMed  CAS  Google Scholar 

Hepatocellular integrity

  1. Bacq, Y., Zarka, O., Bréchot, J.-F., Mariotte, N., Vol, S., Tichet, J., Weill, J.: Liver function tests in normal pregnancy: a prospective study of 103 pregnant women and 103 matched controls. Hepatology 1996; 23: 1030–1034

    Article  PubMed  CAS  Google Scholar 

  2. Chemnitz, G., Schmidt, E., Schmidt, F.W., Lobers, J.: Diagnostische und prognostische Bedeutung massiv erhöhter Glutamat-Dehydrogenase-Aktivität im Serum. Dtsch. Med. Wschr. 1984; 109: 1789–1793

    PubMed  CAS  Google Scholar 

  3. Chung, Y.H., Jung, S.A., Song, B.C., Chang, W.Y., Kim, J.A., Song, I.H., Kim, J.W., Choi, W.B., Shong, Y.K., Lee, Y.S., Suh, D.J.: Plasma isocitrate dehydrogenase as a marker of centrilobular hepatic necrosis in patients with hyperthyroidism. J. Clin. Gastroenterol. 2001; 33: 118–122

    Article  PubMed  CAS  Google Scholar 

  4. Cohen, J.A., Kaplan, M.M.: The SGOT/SGPT ratio—an indicator of alcoholic liver disease. Dig. Dis. Sci. 1979; 24: 835–838

    Article  PubMed  CAS  Google Scholar 

  5. Craxi, A., Almasio, P.: Diagnostic approach to liver enzyme elevation. J. Hepatol. 1996; 25 (suppl. 1): 47–51

    PubMed  Google Scholar 

  6. Daniel, S., Ben-Menachem, T., Vasuderan, G., Ma, C.K., Blumenkehl, M.: Prospective evaluation of unexplained chronic liver transaminase abnormalities in asymptomatic and symptomatic patients Amer. J. Gastroenterol 1999; 94: 3010–3014

    Article  CAS  Google Scholar 

  7. Johnson, R.D., O’Connor, M.L., Kerr, R.M.: Extreme serum elevations of aspartate aminotransferase. Amer. J. Gastroenterol. 1995; 90: 1244–1245

    CAS  Google Scholar 

  8. Kamath, P.S.: Clinical approach to the patient with abnormal liver test results Mayo Clin. Proc. 1996; 71: 1089–1096

    Article  PubMed  CAS  Google Scholar 

  9. Kew, M.C.: Serum aminotransferase concentration as evidence of hepatocellular damage. Lancet 2000; 355: 591–592

    Article  PubMed  CAS  Google Scholar 

  10. MacNamara, E., Goldberg, D.M.: Serum enzymes and enzyme profiles in the diagnosis of liver and biliary tract disease. Surv. Dig. Dis. 1985; 3: 165–186

    Article  CAS  Google Scholar 

  11. Nemesanszky, E., Lott, J.A.: Gamma-glutamyltransferase and its isoenzymes: progress and problems. Clin. Chem. 1985; 31: 797–803

    PubMed  CAS  Google Scholar 

  12. Pratt, D.S., Kaplan, M.M.: Evaluation of abnormal liver enzyme results in asymptomatic patients. New Engl. J. Med. 2000; 342: 1266–1271

    Article  PubMed  CAS  Google Scholar 

  13. Prieto, J., Barry, M., Sherlock, S.: Serum ferritin in patients with iron overload and with acute and chronic liver disease. Gastroenterology 1975; 68: 525–533

    PubMed  CAS  Google Scholar 

  14. Puukka, K., Hietala, J., Koivisto, H., Anttila, P., Bloigu, R., Niemelä, O.: Additive effects of moderate drinking and obesity on serum ψ-glutamyl transferase activity. Amer. J. Clin. Nutr. 2006; 83: 1351–1354

    PubMed  CAS  Google Scholar 

  15. Sherwood, P., Lyburn, I., Brown, S., Ryder, S.: How are abnormal results for liver function tests dealt with in primary care? Audit of yield and impact Brit. Med. J. 2001; 322: 276–278

    Article  PubMed  CAS  Google Scholar 

  16. Sheth, S.G., Flamm, St.L., Gordon, F.D., Chopra, S.: AST/ALT ratio predicts cirrhosis in patients with chronic hepatitis C virus infection. Amer. J. Gastroenterol. 1998; 93: 44–48

    Article  CAS  Google Scholar 

  17. Skelly, M.M., James, P.D., Ryder, S.D.: Findings on liver biopsy to investigate abnormal liver function tests in the abscence of diagnostic serology. J. Hepatol. 2001; 35: 195–199

    Article  PubMed  CAS  Google Scholar 

  18. Whitehead, M.W., Hawkes, N.D., Hainsworth, I., Kingham, J.G.C.: A prospective study of the causes of notably raised aspartate aminotransferase of liver origin. Gut 1999; 45: 129–133

    Article  PubMed  CAS  Google Scholar 

  19. Xu, K., Meng, X.-Y., Wu, J.-W., Shen, B., Shi, Y.-C., Wei, Q.: Diagnostic value of serum ψ-glutamyl-transferase isoenzyme for hepatocellular carcinoma: a 10-year study. Amer. J. Gastroenterol. 1992; 87: 991–995

    CAS  Google Scholar 

Disorders of excretion

  1. Cravetto, C., Molino, G., Biondi, A.M., Cavanna, A., Avagnina, P., Frediani, S.: Evaluation of the diagnostic value of serum bile acid in detection and functional assessment of liver diseases. Ann Clin. Biochem. 1985; 22: 596–605

    PubMed  Google Scholar 

  2. Fukano, M., Amano, S., Hazama, F., Hosoda, S.: 5′-nucleotidase activities in sera and liver tissues of viral hepatitis patients. Gastroenterol. Japon. 1990; 25: 199–205

    CAS  Google Scholar 

  3. Komoda, T., Koyama, I., Nagata, A., Sakagishi, Y., DeSchryver-Kescskemeti, K., Alpers, D.H.: Ontogenic and phylogenic studies of intestinal, hepatic, and placental alkaline phosphatases. Gastroenterology 1986; 91: 277–286

    PubMed  CAS  Google Scholar 

  4. Mannes, G.A., Thieme, C., Stellard, F., Wang, T., Sauerbruch, T., Paumgartner, G.: Prognostic significance of serum bile acids in cirrhosis. Hepatology 1986; 6: 50–53

    Article  PubMed  CAS  Google Scholar 

  5. Moss, D.W.: Alkaline phosphatase isoenzymes. Clin. Chem. 1982; 28: 2007–2016

    PubMed  CAS  Google Scholar 

  6. Rosalki, S.B., Foo, A.Y., Dooley, J.S.: Benign familial hyperphosphatasaemie as a cause of unexplained increase in plasma alkaline phosphatase activity. J. Clin. Pathol. 1993; 46: 738–741

    Article  PubMed  CAS  Google Scholar 

Disorders of synthesis capacity

  1. Bisceglie, A.M., Hoofnagle, J.H.: Elevations in serum alpha-fetoprotein levels in patients with chronic B-hepatitis. Cancer 1989; 64: 2117–2120

    Article  PubMed  Google Scholar 

  2. Brown, S.S., Kalow, W., Pilz, W., Whittacker, M., Woronick, C.L.: The plasma cholinesterase: A new perspective. Adv. Clin. Chem. 1981; 22: 1–123

    Article  PubMed  CAS  Google Scholar 

  3. Kuntz, E.: Die Bestimmung der Serumcholinesterase-Aktivität bei Kranken mit unspezifischen, tuberkulösen und malignen Lungenveränderungen. Med. Welt 1964; 2466–2477

    Google Scholar 

  4. Rondana, M., Milani, L., Merkel, C., Caregaro, L., Gatta, A.: Value of prealbumin plasma levels as liver test. Digestion 1987; 37: 72–78

    Article  PubMed  CAS  Google Scholar 

  5. Walli, A.K., Seidel, D.: Role of lipoprotein-X in the pathogenesis of cholestatic hypercholesterolemia. J. Clin. Invest. 1984; 74: 867–879

    Article  PubMed  CAS  Google Scholar 

Disorders of metabolism

  1. Barsotti, R.J.: Measurement of ammonia in blood. J. Pediatr. 2001; 138 (Suppl.): 11–19

    Article  Google Scholar 

  2. Castell, D.O.: The ammonia tolerance test: an index of portal hypertension. Gastroenterology 1965; 49: 539–543

    PubMed  CAS  Google Scholar 

Diagnostics of liver functions

  1. Aebli, N., Reichen, J.: Prognostische Wertigkeit der seriellen Bestimmung der Galactose-Eliminationsgeschwindigkeit bei chronisch-aktiver Hepatitis. Schweiz. Med. Wschr. 1991; 121: 970–976

    PubMed  CAS  Google Scholar 

  2. Armuzzi, A., Candelli, M., Zocco, M.A., Andreoli, A., de Lorenzo, A., Nista, E.C., Miele, L., Cremonini, F., Cazzato, I.A., Grieco, A., Gasbarrini, G., Gasbarrini, A.: Review article: breath testing for human liver function assessment. Aliment. Pharmacol. Ther. 2002; 16: 1977–1996

    Article  PubMed  CAS  Google Scholar 

  3. Arrigoni, A., Gindro, T., Aimo, G., Cappello, N., Meloni, A., Benedetti, P., Molino, G.P., Verme, G., Rizzetto, M.: Monoethylglycinexylidide test: a prognostic indicator of survival in cirrhosis. Hepatology 1994; 20: 383–387

    PubMed  CAS  Google Scholar 

  4. Bergstrom, M., Soderman, C., Eriksson, L.S.: A simplified method to determine galactose elimination capacity in patients with liver disease. Scand. J. Clin. Lab. Invest. 1993; 53: 667–670

    Article  PubMed  CAS  Google Scholar 

  5. Burns, E., Triger, D.R., Tucker, G.T., Bax, N.D.S.: Indocyanine green elimination in patients with liver disease and in normal subjects. Clin. Sci. 1991; 80: 155–160

    PubMed  CAS  Google Scholar 

  6. Cheng, W.S.C., Murphy, T.L., Smith, M.T., Cooksley, W.G.E., Halliday, J.W., Powell, L.W.: Dose-dependent pharmacokinetics of caffeine in humans: relevance as a test of quantitative liver functions. Clin. Pharmacol. Ther. 1990; 47: 516–524

    PubMed  CAS  Google Scholar 

  7. Erdogan, D., Heijnen, B.H.M., Bennink, R.J., Kok, M., Dinant, S., Straatsburg, I.H., Gouma, D.J., van Gulik, T.M.: Preoperative assessment of liver function: a comparison of (99 m) Tc-Mebrofenin scintigraphy with indocyanine green clearance test. Liver Internat. 2004; 24: 117–123

    Article  CAS  Google Scholar 

  8. Grieco, A., Castellano, R., Matera, A., Marcoccia, S., Di Rocco, P., Ragazoni, E., Vecchio, F.M., Gasbarrini, G.: Antipyrine clearance in chronic and neoplastic liver diseases: a study of 518 patients. J. Gastroenterol. Hepatol. 1998; 13: 460–466

    Article  PubMed  CAS  Google Scholar 

  9. Hemming, A.W., Scudamore, Ch.H., Shackleton, Ch.R., Pudek, M., Erb, S.R.: Indocyanine green clearance as a predictor of successful hepatic resection in cirrhosis patients. Amer. J. Surg. 1992; 163: 515–518

    Article  PubMed  CAS  Google Scholar 

  10. Huang, Y.-S., Lee, S.-D., Deng, J.-F., Wu, J.-C., Lu, R.-H., Lin, Y.-F., Wang, Y.-J., Lo, K.-J.: Measuring lidocaine metabolite — monoethyl-glycinexylidide as a quantitative index of hepatic function in adults with chronic hepatitis and cirrhosis. J. Hepatol. 1993; 19: 140–147

    Article  PubMed  CAS  Google Scholar 

  11. Jalan, R., Hayes, P.C.: Quantitative tests of liver function. Aliment. Pharmacol. Ther. 1995; 9: 263–270

    PubMed  CAS  Google Scholar 

  12. Keiding, S.: Galactose clearance measurements and liver blood flow. Gastroenterology 1988; 94: 477–481

    PubMed  CAS  Google Scholar 

  13. Keiding, S., Engsted, E., Ott, P.: Sorbitol as a test substance for measurement of liver plasma flow in humans. Hepatology 1998; 28: 50–56

    Article  PubMed  CAS  Google Scholar 

  14. Kuntz, H.-D., May, B.: Die Galaktose-Eliminationskapazität. Aussagekraft und klinische Bedeutung. Med. Welt 1983; 34: 646–648

    PubMed  CAS  Google Scholar 

  15. Kuntz, H.-D., May, B.: Quantitative Leberfunktionsdiagnostik. Münch. Med. Wschr. 1983; 125: 678–680

    CAS  Google Scholar 

  16. Kuntz, H.-D., Kuntz, E.: Intravenöse Galaktose-Belastung als Leberfunktionsprobe. Fortschr. Med. 1983; 101: 999–1004

    PubMed  CAS  Google Scholar 

  17. Lewis, F.W., Rector, W.G.: Caffeine clearance in cirrhosis. The value of simplified determinations of liver metabolic capacity. J. Hepatol. 1992; 14: 157–162

    Article  PubMed  CAS  Google Scholar 

  18. Lotterer, E., Högel, J., Gaus, W., Fleig, W.E., Bircher, J.: Quantitative liver function tests as surrogate markers for end-points in controlled clinical trials: a retrospective feasibility study. Hepatology 1997; 26: 1426–1433

    Article  PubMed  CAS  Google Scholar 

  19. Marchesini, G., Fabbri, A., Bugianesi, E., Bianchi, G.P., Marchi, E., Zoli, M., Pisi, E.: Analysis of the deterioration rates of liver function in cirrhosis, based on galactose elimination capacity. Liver 1990; 10: 65–71

    PubMed  CAS  Google Scholar 

  20. McDonagh, J.E., Nathan, V.V., Bonavia, I.C., Moyle, G.R., Tanner, A.R.: Caffeine clearance by enzyme multiplied immunoassay technique: a simple, inexpensive, and useful indicator of liver function. Gut 1991; 32: 681–684

    Article  PubMed  CAS  Google Scholar 

  21. Merkel, C., Gatta, A., Zoli, M., Bolognesi, M., Angeli, P., Iervese, T., Marchesini, G., Ruol, A.: Prognostic value of galactose elimination capacity, aminopyrine breath test, and ICG clearance in patients with cirrhosis Comparison with the Pugh score. Dig. Dis. Sci. 1991; 36: 1197–1203

    Article  PubMed  CAS  Google Scholar 

  22. Merkel, C., Bolognesi, M., Bellon, S., Bianco, S., Honisch, B., Lampe, H., Angeli, P., Gatta, A.: Aminopyrine breath test in the proguostic of patients with cirrhosis. Gut 1992; 33: 836–842

    Article  PubMed  CAS  Google Scholar 

  23. Merkel, C., Marchesini, G., Fabbri, A., Bianco, S., Bianchi, G., Enzo, E., Sacerdoti, D., Zoli, M., Gatta, A.: The course of galactose elimination capacity in patients with alcoholic cirrhosis: possible use as a surrogate marker for death. Hepatology 1996; 24: 820–823

    Article  PubMed  CAS  Google Scholar 

  24. Meyer-Wyss, B., Renner, E., Luo, H., Scholer, A.: Assessment of lidocaine metabolite formation in comparison with other quantitative liver function tests. J. Hepatol. 1993; 19: 133–139

    Article  PubMed  CAS  Google Scholar 

  25. Molino, F., Cavanna, A., Avagnini, P., Ballare, M., Torchio, M.: Hepatic clearance of D-sorbitol. Noninvasive test for evaluating functional liver plasma flow. Dig. Dis. Sci. 1987; 32: 753–758

    Article  PubMed  CAS  Google Scholar 

  26. Monroe, P.S., Baker, A.L., Schneider, J.F., Krager, P.S., Klein, P.D., Schoeller, D.: The a minopyrine breath test and serum bile acids reflect histologic severity in chronic hepatitis. Hepatology 1982; 2: 317–322

    PubMed  CAS  Google Scholar 

  27. Moseley, R.H.: Evaluation of abnormal liver function tests. Med. Clin. N. Amer. 1996; 80: 887–906

    PubMed  CAS  Google Scholar 

  28. Mukherjee, S., Rogers, M.A., Buniak, B.: Comparison of indocyanine green clearance with Child’s-Pugh score and hepatic histology: A multivariate analysis. Hepato-Gastroenterol. 2006; 53: 120–123

    Google Scholar 

  29. Park, G.J.H., Katelaris, P.-H., Jones, D.B., Seow, F., Le Couteur, D.G., Ngu, M.C.: Validity of the (13)C-caffeine breath test as a noninvasive quantitative test of liver function. Hepatology 38: 2003: 1227–1236

    Google Scholar 

  30. Salerno, F., Borroni, G., Moser, P., Sangiovanni, A., Almasio, P., Budillon, G., Capuano, G., Murala, M., Marchesini, G., Bernardi, M., Marenco, G., Molino, G., Rossaro, L., Solinas, A., Ascione, A.: Prognostic value of the galactose test in predicting survival of patients with cirrhosis evaluated for liver transplantation. A prospective multicenter Italian study. J. Hepatol. 1996; 25: 474–480

    Article  PubMed  CAS  Google Scholar 

  31. Testa, R., Caglieris, S., Risso, D., Arzani, L., Campo, N., Alvarez, S., Giannini, E., Lantieri, P.B., Celle, G.: Monoethylglycinexylidide formation measurement as a hepatic function test to assess severity of chronic liver disease. Amer. J. Gastroenterol. 1997; 92: 2268–2273

    CAS  Google Scholar 

  32. Urbain, D., Muls, V., Thys, O., Ham, H.R.: Aminopyrine breath test improver long-term prognostic evaluation in patients with alcoholic cirrhosis in Child class A and B. J. Hepatol. 1995; 22: 179–183

    Article  PubMed  CAS  Google Scholar 

  33. Wang, T., Kleber, G., Stellaard, F., Paumgartner, G.: Caffeine elimination: a test of liver function. Klin. Wschr. 1985; 63: 1124–1128

    Article  PubMed  CAS  Google Scholar 

  34. Williams, S.J., Farrell, G.C.: Serial antipyrine clearance studies defect altered hepatic metabolic function during spontaneous and interferon-induced changes in chronic hepatitis B disease activity. Hepatology 1989; 10: 192–197

    Article  PubMed  CAS  Google Scholar 

  35. Zoedler, T., Ebener, C., Becker, H., Roeher, H.D.: Evaluation of liver function tests to predict operative risk in liver surgery. HPB Surgery 1995; 9: 13–18

    Article  PubMed  CAS  Google Scholar 

Mesenchymal reaction

  1. Bayerdörffer, E., Lamerz, R., Fliege, R., Köpcke, W., Mannes, G.A.: Predictive value of serum procollagen-III-peptide for the survival of patients with cirrhosis. J. Hepatol. 1991; 13: 298–304

    Article  PubMed  Google Scholar 

  2. Bell, H., Raknerud, N., Orjaseter, H., Haug, E.: Serum procollagen III peptide in alcoholic and other chronic liver diseases. Scand. J. Gastroenterol. 1989; 24: 1217–1222

    Article  PubMed  CAS  Google Scholar 

  3. Beukers, R., Zanten, van, R.A.A., Schalm, S.W.: Serial determination of type III procollagen amino propeptide serum levels in patients with histologically progressive and non-progressive primary biliary cirrhosis. J. Hepatol. 1992; 14: 22–29

    Article  PubMed  CAS  Google Scholar 

  4. Fayol, V., Hassanein, H.I., El-Badrawy, N., Ville, G., Hartmann, D.J.: Aminoterminal propeptide of type. III procollagen: a marker of disease activity in schistosomal patients. Eur. J. Clin. Chem. Clin. Biochem. 1991; 29: 737–741

    PubMed  CAS  Google Scholar 

  5. Lotterer, E., Gressner, A.M., Kropf, J., Grobe, E., Knebel, von, D., Bircher, J.: Higher levels of serum aminoterminal type III procollagen peptide, and laminin in alcoholic than in nonalcoholic cirrhosis of equal severity. J. Hepatol. 1992; 14: 71–77

    Article  PubMed  CAS  Google Scholar 

  6. Müller, A., Krombholz, B., Pott, G., Machnik, G., Vollandt, R., Reinhardt, M., Jorke, D.: Collagen peptidase and type III procollagen peptide serum levels in chronic liver diseases. Clin. Chim. Acta 1991; 197: 59–66

    Article  PubMed  Google Scholar 

  7. Niemelä, O., Risteli, J., Blake, J.E., Risteli, L., Compton, K.V., Orego, H.: Markers of fibrogenesis and basement membrane formation in alcoholic liver disease. Relation to severity, presence of hepatitis, and alcohol intake. Gastroenterology 1990; 98: 1612–1619

    PubMed  Google Scholar 

  8. Trinchet, J.-C., Hartmann, D.J., Pateron, D., Laarif, M., Callard, P., Ville, G., Beaugrand, M.: Serum type I collagen and N-terminal peptide of type III procollagen in chronic hepatitis. J. Hepatol. 1991; 12: 139–144

    Article  PubMed  CAS  Google Scholar 

  9. Vuitton, D.A., Seilles, E., Cozon, G., Rossel, M., Bresson-Hadni, S., Revillard, J.P.: Secretory immunoglobulin A in hepatobiliary diseases. Dig. Dis. 1991; 9: 78–91

    Article  PubMed  CAS  Google Scholar 

  10. Wolf, P.L.: Interpretation of electrophoretic patterns of serumproteins. Clin. Lab. Med. 1986; 6: 441–455

    PubMed  CAS  Google Scholar 

Serological diagnostics

  1. Berasain, C., Betés, M., Panizo, A., Ruiz, J., Herrero, J.I., Civeira, M.-P., Prieto, J.: Pathological and virological findings in patients with persistent hypertransaminasaemia of unknown aetiologie. Gut 2000; 47: 429–435

    Article  PubMed  CAS  Google Scholar 

  2. Blum, H.E., Weizsäcker, von, F.: Der HBsAg-negative, anti-HBc/anti-HBs-positive Patient. Internist 1991; 32: 262–266

    PubMed  CAS  Google Scholar 

  3. Blumberg, B.S., Alter, H.J., Visnich, S.: A “new” antigen in leukemia sera. J. Amer. Med. Ass. 1965; 191: 541–546

    CAS  Google Scholar 

  4. Caldwell, St.H., Jeffers, L.J., Ditomaso, A., Millar, A., Clark, R.M., Rabassa, A., Reddy, K.R., De Medina, M., Schiff, E.R.: Antibody to hepatitis C is common among patients with alcoholic liver disease with and without risk factors. Amer. J. Gastroenterol. 1991; 86: 1219–1223

    CAS  Google Scholar 

  5. Choo, Q.L., Kuo, G., Weiner, A.J., Overby, L.R., Bradley, D.W., Houghton, M.: Isolation of a cDNA clone derived from a blood-borne Non-A, Non-B viral hepatitis genome. Science 1989; 244: 359–361

    Article  PubMed  CAS  Google Scholar 

  6. Gerber, M.A., Thung, S.N.: Molecular and cellular pathology of hepatitis B. Lab. Invest. 1985; 52: 572–590

    PubMed  CAS  Google Scholar 

  7. Grob, P.J.: Hepatitisserologie: Anwendung und Interpretation. Therapeut. Umschau 1992; 49: 287–301

    CAS  Google Scholar 

  8. Houghton, M., Weiner, A., Han, J., Kuo, G., Choo, Q.L.: Molecular biology of the hepatitis C viruses implications for diagnosis, development and control of viral disease. Hepatology 1991; 14: 381–388

    Article  PubMed  CAS  Google Scholar 

  9. Najarian, R., Caput, D., Gee, W., Potter, S.J., Renard, A., Merry-weather, J., van Nest, G., Dino, D.: Primary structure and gene organization of human hepatitis A virus. Proc. Nat. Acad. Sci. 1985; 82: 2627–2631

    Article  PubMed  CAS  Google Scholar 

  10. Ray, R., Aggarwal, R., Salunke, P.N., Mehrotra, N.N., Talwar, G.P., Naik, S.R.: Hepatitis E virus genome in stools of hepatitis patients during large epidemic in North India. Lancet 1991; 338: 783–784

    Article  PubMed  CAS  Google Scholar 

  11. Reyes, G.R., Purdy, M.A., Kim, J.P., Luk, K.-C., Young, L.M., Fry, K.E., Bradley, D.W.: Isolation of a cDNA from the virus responsible for enterically transmitted non-A, non-B hepatitis. Science 1990; 247: 1335–1339

    Article  PubMed  CAS  Google Scholar 

  12. Rizzetto, M., Verme, G.: Delta hepatitis-present status. J. Hepatol. 1985; 1: 187–193

    Article  PubMed  CAS  Google Scholar 

  13. Roggendorf, M.: Hepatitis-C-Virus. Dtsch. Med. Wschr. 1990; 115: 352–354

    PubMed  CAS  Google Scholar 

  14. Seelig, H.P.: Polymerase-Kettenreaktion und Hepatitis B. Dtsch. Med. Wschr. 1993; 118: 277–279

    PubMed  CAS  Google Scholar 

  15. Shiina, S., Fujino, H., Kawabe, T., Tagawa, K., Unuma, T., Yoneyama, M., Ohmori, T., Suzuki, S., Kurita, M., Ohashi, Y.: Relationship of HBsAg subtypes with HBeAg/anti-HBe status and chronic liver disease. Part II: Evaluation of epidemiological factors and suspected risk factors of liver dysfunktion. Amer. J. Gastroenterol. 1991; 86: 872–875

    CAS  Google Scholar 

  16. Shiina, S., Fujino, H., Uta, Y., Tagawa, K., Unuma, T., Yoneyama, M., Ohmori, T., Suzuki, S., Kurita, M., Ohashi, Y.: Relationship of HBsAg subtypes with HBeAg/anti-HBe status and chronic liver disease. Part I: Analysis of 1744 HBsAg carriers. Amer. J. Gastroenterol. 1991; 86: 866–871

    CAS  Google Scholar 

  17. Strobel, E., Schöniger, M.: False-positive hepatitis serology after administration of immunoglobulins. Dtsch. Med. Wschr. 2006; 131: 1325–1327

    PubMed  CAS  Google Scholar 

  18. Velazquez, O., Stetler, H.C., Avila, C., Ornelas, G., Alvarez, C., Hadler, S.c., Bradley, D.W., Sepulveda, J.: Epidemic transmission of enterically transmitted non-A, non-B hepatitis in Mexico, 1986–1987. J. Amer. Med. Ass. 1990; 263: 3291–3285

    Google Scholar 

  19. Weston, S.R., Martin, P.: Serological and molecular testing in viral hepatitis: an update. Can. J. Gastroenterol. 2001; 15: 177–184

    PubMed  CAS  Google Scholar 

  20. Yarbourgh, P.O., Tam, A.W., Fry, K.E., Krawczynski, K., McCaustland, K.A., Bradley, D.W., Reyes, G.R.: Hepatitis E virus: identification of type-common epitopes. J. Viral. 1991; 65: 5790–5797

    Google Scholar 

Immunological diagnostics

  1. Berg, P.A., Klein, R.: Antimitochondrial antibodies in primary biliary cirrhosis and other disorders: definition and clinical relevance. Dig. Dis. 1992; 10: 85–101

    Article  PubMed  CAS  Google Scholar 

  2. Czaja, A.J., Manns, M.P., Homburger, H.A.: Frequency and significance of antibodies to liver/kidney microsome Type 1 in adults with chronic active hepatitis. Gastroenterology 1992; 103: 1290–1295

    PubMed  CAS  Google Scholar 

  3. Czaja, A.J., Homburger, H.A.: Autoantibodies in liver disease. Gastroenterology 2001; 120: 239–249

    Article  PubMed  CAS  Google Scholar 

  4. Duerr, R.H., Targan, S.R., Landers, C.J., LaRusso, N.F., Lindsay, K.L., Wiesner, F.H., Shanahan, F.: Neutrophil cytoplasmatic antibodies: a link between primary cholangitis and ulcerative colitis. Gastroenterology 1991; 100: 1385–1391

    PubMed  CAS  Google Scholar 

  5. Klein, R., Berg, P.A.: Autoantikörper bei chronischen Lebererkrankungen: klinische und diagnostische Relevanz. Klin. Lab. 1993; 39: 611–626

    Google Scholar 

  6. Laskin, C.A., Vidins, E., Blendis, L.M., Soloninka, Ch.A.: Autoantibodies in alcoholic liver disease. Amer. J. Med. 1990; 89: 129–133

    Article  PubMed  CAS  Google Scholar 

  7. Manns, M., Kyriatsoulis, A., Gerken, G., Staritz, M., Meyer zum Büschenfelde, K.-H.: Characterisation of a new subgroup of autoimmune chronic active hepatitis by autoantibodies against a soluble liver antigen. Lancet 1987/I: 292–294

    Article  Google Scholar 

  8. Manns, M.P., Meyer zum Büschenfelde, K.-H.: Nature of autoantigens and autoantibodies in autoimmune hepatitis. Springer Semin. Immunopath. 1990; 12: 57–65

    CAS  Google Scholar 

  9. Meyer zum Büschenfelde, K.-H., Lohse, A.W., Manns, M., Poralla, T.: Autoimmunity and liver disease. Hepatology 1990; 12: 354–363

    Article  PubMed  Google Scholar 

  10. Penner, E., Müller, S., Zimmermann, D., Van, M.H.V.: High prevalence of antibodies to histones among patients with primary biliary cirrhosis. Clin. Exper. Immunol. 1987; 70: 47–52

    Google Scholar 

  11. Seibold, F., Slametschka, D., Gregor, M., Weber, P.: Neutrophil autoantibodies: a genetic marker in primary sclerosing cholangitis and ulcerative colitis. Gastroenterology 1994; 107: 532–536

    PubMed  CAS  Google Scholar 

  12. Storch, W.B.: New autoantibodies and their antigens in autoimmune diseases. Cell. Molec. Biol. 1997; 43: 337–344

    CAS  Google Scholar 

  13. Storch, W.B.: Immunofluorescence in clinical immunology. A primer and atlas. Birkhäuser (Basel), 2000

    Google Scholar 

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(2008). Laboratory diagnostics. In: Hepatology Textbook and Atlas. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-76839-5_5

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