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Specialized Components of the Translational Machinery for Unnatural Amino Acid Mutagenesis: tRNAs, Aminoacyl-tRNA Synthetases, and Ribosomes

  • Chapter
Protein Engineering

Part of the book series: Nucleic Acids and Molecular Biology ((NUCLEIC,volume 22))

Site-specific incorporation of unnatural amino acids (amino acid analogues) into proteins adds a new dimension to studies of protein structure and function. Here, we describe in detail the development of methods for site-specific incorporation of unnatural amino acids with novel chemical, physical and biological properties using specialized suppressor tRNAs alongside engineered aminoacyl-tRNA synthetases and ribosomes.

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    C. Köhrer & U. L. RajBhandary

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Köhrer, C., RajBhandary, U.L. (2009). Specialized Components of the Translational Machinery for Unnatural Amino Acid Mutagenesis: tRNAs, Aminoacyl-tRNA Synthetases, and Ribosomes. In: Köhrer, C., RajBhandary, U.L. (eds) Protein Engineering. Nucleic Acids and Molecular Biology, vol 22. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-70941-1_7

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