Abstract
This chapter describes the measurement of plasma renin activity by calculation of the quantity of angiotensin I that is released in plasma by the action of endogenous enzyme renin on angiotensinogen, the endogenous substrate. Details are given for the generation of angiotensin I from plasma using controlled pH and temperature conditions. The generated angiotensin I is then quantified by a radioimmunoassay using anti-angiotensin I antibody and iodinated angiotensin I as label. Separation of the antibody-bound fraction from the unbound is achieved using dextran-coated charcoal. Additional notes describe critical steps in the assay. The correct procedures for sample handling to overcome the potential problems of cryoactivation of prorenin to renin are described.
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References
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Barnes, S.C. (2013). Measurement of Plasma Renin Activity. In: Wheeler, M. (eds) Hormone Assays in Biological Fluids. Methods in Molecular Biology, vol 1065. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-616-0_15
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DOI: https://doi.org/10.1007/978-1-62703-616-0_15
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-615-3
Online ISBN: 978-1-62703-616-0
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