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Translating Spermatogonial Stem Cell Transplantation to the Clinic

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Male Germline Stem Cells: Developmental and Regenerative Potential

Part of the book series: Stem Cell Biology and Regenerative Medicine ((STEMCELL))

Abstract

The spermatogenic lineage is maintained by a pool of spermatogonial stem cells (SSCs) that balance self-renewing and differentiating divisions to meet the biological demand of the testis. In animal models (mice, rats, goats, pigs, and dogs), SSCs transplanted into infertile recipient testes exhibit the remarkable ­potential to regenerate spermatogenesis. Tremendous progress has been made towards applying SSC transplantation techniques to primates (human and nonhuman), which may have applicability in the human fertility clinic. This chapter will review the approaches used to isolate and preserve donor SSCs from primate testes, ­generate infertile recipient nonhuman primates for transplantation ­studies, ­transplant donor SSCs into primate testes, and evaluate transplant outcomes. In addition, we discuss some of the clinical considerations for future application of these techniques in patients.

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Acknowledgments

Work performed in our laboratory was supported by the Magee-Womens Research Institute and Foundation, the Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD/NIH) through cooperative agreement (U54 HD08160) as part of the Specialized Cooperative Centers Program in Reproduction and Infertility Research, NIH grants RR018500, AG024992, and HD055475 to KEO, an institutional NRSA postdoctoral ­fellowship (HD007332) and Pathways to Independence Award (HD062687) to BPH. We are also grateful to Tony Plant for providing monkey testicular tissue for our fundamental developmental biology studies (HD013254 to TMP). Artwork in Fig. 11.1 was produced by Molly Feuer (http://www.feuerillustration.com).

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Authors and Affiliations

  1. Department of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womens Research Institute, University of Pittsburgh School of Medicine, 204 Craft Avenue, Pittsburgh, PA, 15213, USA

    Brian P. Hermann & Kyle E. Orwig

  2. Department of Biology, The University of Texas at San Antonio, San Antonio, USA

    Brian P. Hermann

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  1. Brian P. Hermann
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  2. Kyle E. Orwig
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Correspondence to Brian P. Hermann or Kyle E. Orwig .

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Editors and Affiliations

  1. Magee-Womens Research Institute, University of Pittsburgh, Craft Avenue 204, Pittsburgh, 15213, Pennsylvania, USA

    Kyle E. Orwig

  2. Magee-Womens Research Institute, University of Pittsburgh, Craft Avenue 204, Pittsburgh, 15213, Pennsylvania, USA

    Brian P. Hermann

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© 2011 Springer Science+Business Media, LLC

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Hermann, B.P., Orwig, K.E. (2011). Translating Spermatogonial Stem Cell Transplantation to the Clinic. In: Orwig, K., Hermann, B. (eds) Male Germline Stem Cells: Developmental and Regenerative Potential. Stem Cell Biology and Regenerative Medicine. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61737-973-4_11

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