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Looking Beyond HEA: Matching SCD Patients for RH Variants

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BeadChip Molecular Immunohematology

Abstract

Red blood cell (RBC) blood group alloimmunization remains a major problem in transfusion medicine. This is particularly true for chronically transfused patients. Patients with sickle cell disease (SCD) notoriously make more alloantibodies to RBC antigens than any other patient population, especially alloantibodies to Rh antigens. The classical method of testing for blood group antigens and antibodies is hemagglutination. However, this method has certain limitations, some of which can be overcome by testing DNA to predict a blood type. DNA testing allows conservation of antibodies for confirmation by hemagglutination of predicted antigen negativity. High-throughput DNA array platforms allow for the testing of a relatively large number of donors, thereby providing antigen-negative blood to patients in a novel way and possibly preventing immunization. We performed a study to compare the results obtained by using RH BeadChips™ to laboratory-developed tests and to hemagglutination. Two sets of samples were tested: one set chosen had variant Rh types to challenge the BeadChip™ technology. The other set consisted of random samples from African-American donors and SCD patients to estimate the prevalence of RH alleles. The purpose of this testing was to assess the accuracy and efficiency of the BioArray Solutions prototype BeadChip™ system for RH allele determination.

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Authors and Affiliations

  1. Laboratory of Immunochemistry, New York Blood Center, New York, NY, USA

    Marion E. Reid

Authors
  1. Marion E. Reid
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  2. Christine Halter Hipsky
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Correspondence to Marion E. Reid .

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Editors and Affiliations

  1. Transfusion Medicine Div., Johns Hopkins Hospital, N. Wolfe St. 600, Baltimore, 21287-6667, Maryland, USA

    Paul M. Ness

  2. Pediatric Transfusion Medicine, Children's Hospital Boston, Longwood Avenue 300, Boston, 02115, Massachusetts, USA

    Steve R. Sloan

  3. Scientific Support Services, LifeShare Blood Centers, Linwood Avenue 8910, Shreveport, 71106, Louisiana, USA

    JoAnn M. Moulds

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Reid, M.E., Hipsky, C.H. (2011). Looking Beyond HEA: Matching SCD Patients for RH Variants. In: Ness, P., Sloan, S., Moulds, J. (eds) BeadChip Molecular Immunohematology. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-7512-6_10

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