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Development and Use of Fluorescent Nanodiamonds as Cellular Markers

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Nanodiamonds

Abstract

Diamond is an allotrope of carbon. A unique property that distinguishes it from the other carbon materials is that diamond is optically transparent and often contains point defects as color centers. Nitrogen vacancy (N-V) defects are the most noteworthy color centers in diamond. These centers can be produced reproducibly by ion beam irradiation, followed by thermal annealing, and can emit strong and stable fluorescence when excited by visible light. This unique optical property combined with the non-cytotoxicity and good surface functionalizability characteristics of the material makes nanoscale diamonds a promising fluorescent probe for bioimaging applications in cellular environments. This article summarizes the results of our efforts in production and characterization of bright, multicolored (red and green) fluorescent nanodiamonds (FNDs) and their use as cellular markers. Notable advancement of technologies along this line includes mass production of FNDs and real time tracking of a single 35-nm red FND particle in three dimensions in live cells. We envision that further development of the material will provide an increased sensitivity and improved capability for fruitful applications of FNDs in biology and medicine.

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Acknowledgment

The author thanks Y.-R. Chang and H.-Y. Chou for their assistance in preparing this manuscript. This research was supported by the Academia Sinica and the National Science Council (Grant No. NSC 96-2120-M-001-008-) of Taiwan, ROC.

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Authors and Affiliations

  1. Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, 106, Taiwan

    Huan-Cheng Chang

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  1. Huan-Cheng Chang
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Correspondence to Huan-Cheng Chang .

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Editors and Affiliations

  1. Dept. Biomedical Engineering , Northwestern University, Sheridan Road 2145, Evanston, 60208-3107, U.S.A.

    Dean Ho

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This article is dedicated to the memory of Wunshain Fann (1961–2008).

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Chang, HC. (2010). Development and Use of Fluorescent Nanodiamonds as Cellular Markers. In: Ho, D. (eds) Nanodiamonds. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-0531-4_6

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  • DOI: https://doi.org/10.1007/978-1-4419-0531-4_6

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