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Pancreatic Cell Differentiation

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Human Cell Culture

Part of the book series: Human Cell Culture ((HUCC,volume 6))

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References

  • Abraham, E.J., Leech, C.A., Lin, J.C., Zulewski, H., and Habener, J.F. (2002) Insulinotropic hormone glucagon-like peptide-1 differentiation of human pancreatic islet-derived progenitor cells into insulin-producing cells. Endocrinology, 143: 3152–3161.

    Article  PubMed  CAS  Google Scholar 

  • Altmann, C.R., Chow, R.L., Lang, R.A., and Hemmati-Brivanlou, A. (1997) Lens induction by Pax-6 in Xenopus laevis. Dev. Biol., 185: 119–123.

    Article  PubMed  CAS  Google Scholar 

  • Apelqvist, A., Li, H., Sommer, L., et al. (1999) Notch signalling controls pancreatic cell differentiation. Nature, 400: 877–881.

    Article  PubMed  CAS  Google Scholar 

  • Assady, S., Maor, G., Amit, M., et al. (2001) Insulin production by human embryonic stem cells. Diabetes, 50: 1691–1697.

    Article  PubMed  CAS  Google Scholar 

  • Bai, L., Meredith, G., and Tuch, B.E. (2005) Glucagon-like peptide-1 enhances production of insulin in insulin-producing cells derived from mouse embryonic stem cells. J. Endocrinol., 186: 343–352.

    Article  PubMed  CAS  Google Scholar 

  • Berna, G., Leon-Quinto, T., Ensenat-Waser, R., et al. (2001) Stem cells and diabetes. Biomed. Pharmacother., 55: 206–212.

    Article  PubMed  CAS  Google Scholar 

  • Blyszczuk, P., Asbrand, C., Rozzo, A., et al. (2004) Embryonic stem cells differentiate into insulin-producing cells without selection of nestin-expressing cells. Int. J. Dev. Biol., 48: 1095–1104.

    Article  PubMed  CAS  Google Scholar 

  • Blyszczuk, P., Czyz, J., Kania, G., et al. (2003) Expression of Pax4 in embryonic stem cells promotes differentiation of nestin-positive progenitor and insulin-producing cells. Proc. Natl. Acad. Sci. USA, 100: 998–1003. Epub 2003 Jan 13.

    Article  PubMed  CAS  Google Scholar 

  • Bonner-Weir, S., Taneja, M., Weir, G.C., et al. (2000) In vitro cultivation of human islets from expanded ductal tissue. Proc. Natl. Acad. Sci. USA, 97: 7999–8004.

    Article  PubMed  CAS  Google Scholar 

  • Brolen, G.K., Heins, N., Edsbagge, J., and Semb, H. (2005) Signals from the embryonic mouse pancreas induce differentiation of human embryonic stem cells into insulin-producing beta-cell-like cells. Diabetes, 54: 2867–2874.

    Article  PubMed  CAS  Google Scholar 

  • Chiang, M.K. and Melton, D.A. (2003) Single-cell transcript analysis of pancreas development. Dev. Cell, 4: 383–393.

    Article  PubMed  CAS  Google Scholar 

  • Colman, A. (2004) Making new beta cells from stem cells. Semin. Cell Dev. Biol., 15: 337–345.

    Article  PubMed  CAS  Google Scholar 

  • D'Amour, K.A., Agulnick, A.D., Eliazer, S., et al. (2005) Efficient differentiation of human embryonic stem cells to definitive endoderm. Nat. Biotechnol., 23: 1534–1541. Epub 2005 Oct 28.

    Article  PubMed  Google Scholar 

  • David, R., Groebner, M., and Franz, W.M. (2005) Magnetic cell sorting purification of differentiated embryonic stem cells stably expressing truncated human CD4 as surface marker. Stem Cells, 23: 477–482.

    Article  PubMed  CAS  Google Scholar 

  • Edlund, H. (2001) Developmental biology of the pancreas. Diabetes, 50: S5–S9.

    Article  PubMed  CAS  Google Scholar 

  • Ferber, S., Halkin, A., Cohen, H., et al. (2000) Pancreatic and duodenal homeobox gene 1 induces expression of insulin genes in liver and ameliorates streptozotocin-induced hyperglycemia. Nat. Med., 6: 568–572.

    Article  PubMed  CAS  Google Scholar 

  • Fujikawa, T., Oh, S.H., Pi, L., et al. (2005) Teratoma formation leads to failure of treatment for type I diabetes using embryonic stem cell-derived insulin-producing cells. Am. J. Pathol., 166: 1781–1791.

    PubMed  CAS  Google Scholar 

  • Gradwohl, G., Dierich, A., LeMeur, M., and Guillemot, F. (2000) Neurogenin3 is required for the development of the four endocrine cell lineages of the pancreas. Proc. Natl. Acad. Sci. USA, 97: 1607–1611.

    Article  PubMed  CAS  Google Scholar 

  • Grapin-Botton, A., Majithia, A.R., and Melton, D.A. (2001) Key events of pancreas formation are triggered in gut endoderm by ectopic expression of pancreatic regulatory genes. Genes Dev., 15: 444–454.

    Article  PubMed  CAS  Google Scholar 

  • Gu, G., Dubauskaite, J., and Melton, D.A. (2002) Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors. Development, 129: 2447–2457.

    PubMed  CAS  Google Scholar 

  • Hansson, M., Tonning, A., Frandsen, U., et al. (2004) Artifactual insulin release from differentiated embryonic stem cells. Diabetes, 53: 2603–2609.

    Article  PubMed  CAS  Google Scholar 

  • Hori, Y., Rulifson, I.C., Tsai, B.C., et al. (2002) Growth inhibitors promote differentiation of insulin-producing tissue from embryonic stem cells. Proc. Natl. Acad. Sci. USA, 99: 16105–16110. Epub 2002 Nov 19.

    Article  PubMed  CAS  Google Scholar 

  • Humphrey, R.K., Bucay, N., Beattie, G.M., et al. (2003) Characterization and isolation of promoter-defined nestin-positive cells from the human fetal pancreas. Diabetes, 52: 2519–2525.

    Article  PubMed  CAS  Google Scholar 

  • Itkin-Ansari, P., Demeterco, C., Bossie, S., et al. (2000) PDX-1 and cell-cell contact act in synergy to promote delta-cell development in a human pancreatic endocrine precursor cell line. Mol. Endocrinol., 14: 814–822.

    Article  PubMed  CAS  Google Scholar 

  • Itskovitz-Eldor, J., Schuldiner, M., Karsenti, D., et al. (2000) Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers. Mol. Med., 6: 88–95.

    PubMed  CAS  Google Scholar 

  • Juang, J.H., Bonner-Weir, S., Ogawa, Y., Vacanti, J.P., and Weir, G.C. (1996) Outcome of subcutaneous islet transplantation improved by polymer device. Transplantation, 61: 1557–1561.

    Article  PubMed  CAS  Google Scholar 

  • Kahan, B.W., Jacobson, L.M., Hullett, D.A., et al. (2003) Pancreatic precursors and differentiated islet cell types from murine embryonic stem cells: an in vitro model to study islet differentiation. Diabetes, 52: 2016–2024.

    Article  PubMed  CAS  Google Scholar 

  • Kimura, K., Hattori, S., Kabuyama, Y., et al. (1994) Neurite outgrowth of PC12 cells is suppressed by wortmannin, a specific inhibitor of phosphatidylinositol 3-kinase. J. Biol. Chem., 269: 18961–18967.

    PubMed  CAS  Google Scholar 

  • Klug, M.G., Soonpaa, M.H., Koh, G.Y., and Field, L.J. (1996) Genetically selected cardiomyocytes from differentiating embronic stem cells form stable intracardiac grafts. J. Clin. Invest., 98: 216–224.

    Article  PubMed  CAS  Google Scholar 

  • Ku, H.T., Zhang, N., Kubo, A., et al. (2004) Committing embryonic stem cells to early endocrine pancreas in vitro. Stem Cells, 22: 1205–1217.

    Article  PubMed  Google Scholar 

  • Kubo, A., Shinozaki, K., Shannon, J.M., et al. (2004) Development of definitive endoderm from embryonic stem cells in culture. Development, 131: 1651–1662. Epub 2004 Mar 3.

    Article  PubMed  CAS  Google Scholar 

  • Lammert, E., Cleaver, O., and Melton, D. (2001) Induction of pancreatic differentiation by signals from blood vessels. Science, 294: 564–567. Epub 2001 Sep 27.

    Article  PubMed  CAS  Google Scholar 

  • Leon-Quinto, T., Jones, J., Skoudy, A., Burcin, M., and Soria, B. (2004) In vitro directed differentiation of mouse embryonic stem cells into insulin-producing cells. Diabetologia, 47: 1442–1451. Epub 2004 Jul 29.

    Article  PubMed  CAS  Google Scholar 

  • Lukowiak, B., Vandewalle, B., Riachy, R., et al. (2001) Identification and purification of functional human beta-cells by a new specific zinc-fluorescent probe. J. Histochem. Cytochem., 49: 519–528.

    PubMed  CAS  Google Scholar 

  • Lumelsky, N., Blondel, O., Laeng, P., et al. (2001) Differentiation of embryonic stem cells to insulin-secreting structures similar to pancreatic islets. Science, 292: 1389–1394. Epub 2001 Apr 26.

    Article  PubMed  CAS  Google Scholar 

  • Mandrup-Poulsen, T. (1998) Diabetes. BMJ, 316: 1221–1225.

    PubMed  CAS  Google Scholar 

  • Meyer, C., Hering, B.J., Grossmann, R., et al. (1998) Improved glucose counterregulation and autonomic symptoms after intraportal islet transplants alone in patients with long-standing type I diabetes mellitus. Transplantation, 66: 233–240.

    Article  PubMed  CAS  Google Scholar 

  • Micallef, S.J., Janes, M.E., Knezevic, K., et al. (2005) Retinoic acid induces Pdx1-positive endoderm in differentiating mouse embryonic stem cells. Diabetes, 54: 301–305. Epub 2004 Dec 7.

    Article  PubMed  CAS  Google Scholar 

  • Miyazaki, S., Yamato, E., and Miyazaki, J. (2004) Regulated expression of pdx-1 promotes in vitro differentiation of insulin-producing cells from embryonic stem cells. Diabetes, 53: 1030–1037.

    Article  PubMed  CAS  Google Scholar 

  • Moritoh, Y., Yamato, E., Yasui, Y., Miyazaki, S., and Miyazaki, J. (2003) Analysis of insulin-producing cells during in vitro differentiation from feeder-free embryonic stem cells. Diabetes, 52: 1163–1168.

    Article  PubMed  CAS  Google Scholar 

  • Muller, M., Fleischmann, B.K., Selbert, S., et al. (2000) Selection of ventricular-like cardiomyocytes from ES cells in vitro. FASEB. J., 14: 2540–2548.

    Article  PubMed  CAS  Google Scholar 

  • Ogneva, V. and Martinova, Y. (2002) The effect of in vitro fibroblast growth factors on cell proliferation in pancreas from normal and streptozotocin-treated rats. Diabetes Res. Clin. Pract., 57: 11–16.

    Article  PubMed  CAS  Google Scholar 

  • Otonkoski, T., Beattie, G.M., Mally, M.I., Ricordi, C., and Hayek, A. (1993) Nicotinamide is a potent inducer of endocrine differentiation in cultured human fetal pancreatic cells. J. Clin. Invest., 92: 1459–1466.

    Article  PubMed  CAS  Google Scholar 

  • Paek, H.J., Morgan, J.R., and Lysaght, M.J. (2005) Sequestration and synthesis: the source of insulin in cell clusters differentiated from murine embryonic stem cells. Stem Cells, 23: 862–867. Epub 2005 May 11.

    Article  PubMed  CAS  Google Scholar 

  • Pfendler, K.C., Catuar, C.S., Meneses, J.J., and Pedersen, R.A. (2005) Overexpression of Nodal promotes differentiation of mouse embryonic stem cells into mesoderm and endoderm at the expense of neuroectoderm formation. Stem Cells Dev., 14: 162–172.

    Article  PubMed  CAS  Google Scholar 

  • Ptasznik, A., Beattie, G.M., Mally, M.I., et al. (1997) Phosphatidylinositol 3-kinase is a negative regulator of cellular differentiation. J. Cell Biol., 137: 1127–1136.

    Article  PubMed  CAS  Google Scholar 

  • Quesada, I., Fuentes, E., Andreu, E., et al. (2003) On-line analysis of gap junctions reveals more efficient electrical than dye coupling between islet cells. Am. J. Physiol. Endocrinol. Metab., 284: E980–E987. Epub 2003 Jan 7.

    PubMed  CAS  Google Scholar 

  • Rajagopal, J., Anderson, W.J., Kume, S., Martinez, O.I., and Melton, D.A. (2003) Insulin staining of ES cell progeny from insulin uptake. Science, 299: 363.

    PubMed  Google Scholar 

  • Reubinoff, B.E., Pera, M.F., Fong, C.Y., Trounson, A., and Bongso, A. (2000) Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat. Biotechnol., 18: 399–404.

    Article  PubMed  CAS  Google Scholar 

  • Schuldiner, M., Yanuka, O., Itskovitz-Eldor, J., Melton, D.A., and Benvenisty, N. (2000) Effects of eight growth factors on the differentiation of cells derived from human embryonic stem cells. Proc. Natl. Acad. Sci. USA, 97: 11307–11312.

    Article  PubMed  CAS  Google Scholar 

  • Segev, H., Fishman, B., Ziskind, A., Shulman, M., and Itskovitz-Eldor, J. (2004) Differentiation of human embryonic stem cells into insulin-producing clusters. Stem Cells, 22: 265–274.

    Article  PubMed  CAS  Google Scholar 

  • Shi, Y., Hou, L., Tang, F., et al. (2005) Inducing embryonic stem cells to differentiate into pancreatic beta cells by a novel three-step approach with activin A and all-trans retinoic acid. Stem Cells, 23: 656–662.

    Article  PubMed  CAS  Google Scholar 

  • Shiroi, A., Yoshikawa, M., and Yokota, H., et al. (2002) Identification of insulin-producing cells derived from embryonic stem cells by zinc-chelating dithizone. Stem Cells, 20: 284–292.

    Article  PubMed  CAS  Google Scholar 

  • Sipione, S., Eshpeter, A., Lyon, J.G., Korbutt, G.S., and Bleackley, R.C. (2004) Insulin expressing cells from differentiated embryonic stem cells are not beta cells. Diabetologia, 47: 499–508. Epub 2004 Feb 14.

    Article  PubMed  CAS  Google Scholar 

  • Soria, B. (2001) In-vitro differentiation of pancreatic beta-cells. Differentiation, 68: 205–219.

    Article  PubMed  CAS  Google Scholar 

  • Soria, B., Roche, E., Berna, G., et al. (2000) Insulin-secreting cells derived from embryonic stem cells normalize glycemia in streptozotocin-induced diabetic mice engineering pancreatic islets. Diabetes, 49: 157–162.

    Article  PubMed  CAS  Google Scholar 

  • Soria, B., Skoudy, A., and Martin, F. (2001) From stem cells to beta cells: new strategies in cell therapy of diabetes mellitus. Diabetologia, 44: 407–415.

    Article  PubMed  CAS  Google Scholar 

  • Tada, S., Era, T., Furusawa, C., et al. (2005) Characterization of mesendoderm: a diverging point of the definitive endoderm and mesoderm in embryonic stem cell differentiation culture. Development, 132: 4363–4374. Epub 2005 Sep 1.

    Article  PubMed  CAS  Google Scholar 

  • Tapscott, S.J. (2005) The circuitry of a master switch: myod and the regulation of skeletal muscle gene transcription. Development, 132: 2685–2695.

    Article  PubMed  CAS  Google Scholar 

  • Thomson, J.A. and Marshall, V.S. (1998) Primate embryonic stem cells. Curr. Top. Dev. Biol., 38: 133–165.

    Article  PubMed  CAS  Google Scholar 

  • Thomson, J.A., Itskovitz-Eldor, J., Shapiro, S.S., et al. (1998) Embryonic stem cell lines derived from human blastocysts. Science, 282: 1145–1147.

    Article  PubMed  CAS  Google Scholar 

  • Treutelaar, M.K., Skidmore, J.M., Dias-Leme, C.L., et al. (2003) Nestin-lineage cells contribute to the microvasculature but not endocrine cells of the islet. Diabetes, 52: 2503–2512.

    Article  PubMed  CAS  Google Scholar 

  • Vaca, P., Martin, F., Vegara-Meseguer, J., et al. (2005) Induction of differentiation of embryonic stem cells into insulin secreting cells by fetal soluble factors. Stem Cells, 18: 18.

    Google Scholar 

  • Vallier, L., Reynolds, D., and Pedersen, R.A. (2004) Nodal inhibits differentiation of human embryonic stem cells along the neuroectodermal default pathway. Dev. Biol., 275: 403–421.

    Article  PubMed  CAS  Google Scholar 

  • Weir, G.C. and Bonner-Weir, S. (1998) Islet transplantation as a treatment for diabetes. J. Am. Optom. Assoc., 69: 727–732.

    PubMed  CAS  Google Scholar 

  • Yasunaga, M., Tada, S., Torikai-Nishikawa, S., et al. (2005) Induction and monitoring of definitive and visceral endoderm differentiation of mouse ES cells. Nat. Biotechnol., 23: 1542–1550. Epub 2005 Nov 27.

    Article  Google Scholar 

  • Zalzman, M., Anker-Kitai, L., and Efrat, S. (2005) Differentiation of human liver-derived, insulin-producing cells toward the beta-cell phenotype. Diabetes, 54: 2568–2575.

    Article  PubMed  CAS  Google Scholar 

  • Zhao, M., Christie, M.R., Heaton, N., et al. (2002) Amelioration of streptozotocin-induced diabetes in mice using human islet cells derived from long-term culture in vitro. Transplantation, 73: 1454–1460.

    Article  PubMed  Google Scholar 

  • Zulewski, H., Abraham, E.J., Gerlach, M.J., et al. (2001) Multipotential nestin-positive stem cells isolated from adult pancreatic islets differentiate ex vivo into pancreatic endocrine, exocrine, and hepatic phenotypes. Diabetes, 50: 521–533.

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Institute of Human Genetics, University of Newcastle, Newcastle upon Tyne, UK

    Bettina Fishman

  2. The Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, Israel

    Hanna Segev

  3. The Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, Israel

    Joseph Itskovitz-Eldor

Authors
  1. Bettina Fishman
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  2. Hanna Segev
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  3. Joseph Itskovitz-Eldor
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Editor information

Editors and Affiliations

  1. University College London, London, UK

    John R. Masters

  2. University of California, San Diego, CA, USA

    Bernhard O. Palsson

  3. University of Wisconsin Medical School, 470 N. Charter Street, 53706-1509, Madison, WI, USA

    James A. Thomson

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Fishman, B., Segev, H., Itskovitz-Eldor, J. (2007). Pancreatic Cell Differentiation. In: Masters, J.R., Palsson, B.O., Thomson, J.A. (eds) Human Cell Culture. Human Cell Culture, vol 6. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5983-4_11

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