Abstract
Optical imaging using fluorescent probes, along with related biomedical imaging technologies such as PET, SPECT, MRI and bioluminescence, represents a return to in situ observational methods of biological discovery in contrast to the reductionist molecular level genomic and proteomic methods that have dominated biomedical research for the past 50 years. This chapter will delineate and compare the properties of fluorescent probes, which are the molecular constructs responsible for contrast generation in most fluorescence imaging applications. For this reason, fluorescence microscopy and other imaging technologies that can deliver this essential contextual information are of ever-increasing importance in biomedical research. This chapter will delineate and compare the properties of fluorescent probes, which are the molecular constructs responsible for contrast generation in most fluorescence imaging applications.
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Johnson, I. (2010). Fluorophores for Optical Imaging. In: Rosenthal, E., Zinn, K. (eds) Optical Imaging of Cancer. Springer, New York, NY. https://doi.org/10.1007/978-0-387-93874-5_4
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