Changes in activity of regulated genes correlate with the synthesis of the encoded proteins directly affecting the physiological status of the individual. In most of the cases, protein synthesis is directly proportional to the transcriptional activity of its gene, which affects the steady state levels of mRNA encoding the peptide. Thus, measuring mRNA is the obligated first step in investigating how expression of a gene can be regulated.
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- ATP:
-
adenosine triphosphate
- cDNA:
-
complementary DNA
- cRNA:
-
complementary RNA
- CsCL:
-
cesium chloride
- Ct:
-
threshold cycle
- DNA:
-
deoxyribonucleic acid
- dNTP:
-
deoxynucleotide triphosphate
- FRET:
-
fluorescence resonance energy transfer
- GAPDH:
-
glyceral-dehyde-3-phosphate-dehydrogenase
- GTC:
-
guanidinium isothiocyanate
- hnRNA:
-
heterogeneous nuclear RNA
- mRNA:
-
messenger RNA
- oligo-dT:
-
oligonucleotide chain of deoxythimidines
- PCR:
-
polymerase chain reaction
- poly-A:
-
poly-adenylated
- RNA:
-
ribonucleic acid
- rRNA:
-
ribosomal RNA
- RT-PCR:
-
reverse transcriptase polymerase chain reaction
- UTP:
-
uridine triphosphate
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LaVoie, H.A., Chedrese, P.J. (2009). Analyzing Gene Expression. In: Chedrese, P. (eds) Reproductive Endocrinology. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-88186-7_9
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