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Techniques for DNA Analysis

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Reproductive Endocrinology

Molecular biology is a dynamic field with techniques and analytical tools continuously being developed. Many of the fundamental DNA analysis techniques were developed more than 30 years ago and have evolved through various modifications, automation, and computerization. This chapter reviews the basic concepts and techniques in order to understand how the procedures progressed into those used today.

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Author information

Authors and Affiliations

  1. Brain Tumor Biology Unit, University of Navarra, Pamplona, Spain

    Javier S. Castresana

  2. Department of Health Sciences, University of Navarra, Pamplona, Spain

    Paula Lázcoz

Authors
  1. Javier S. Castresana
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  2. Paula Lázcoz
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Corresponding author

Correspondence to Javier S. Castresana .

Editor information

Editors and Affiliations

     

    32P:

    radioactive isotope

    5α-DHT:

    5α–dihydrotestosterone

    BAC:

    bacterial artificial chromosome

    BSP:

    bisulfite sequencing PCR

    CGH:

    comparative genomic hybridization

    CMC:

    chemical mismatch cleavage

    dATP:

    deoxyadenosine triphosphate

    dCTP:

    deoxycytidine triphosphate

    ddATP:

    dideoxyadenosine triphosphate

    ddCTP:

    dideoxycytidine triphosphate

    ddGTP:

    dideoxyguanosine triphosphate

    ddNTP:

    dideoxynucleoside triphosphate

    ddTTP:

    dideoxythymidine triphosphate

    DGGE:

    denaturing gradient gel electrophoresis

    dGTP:

    deoxyguanosine triphosphate

    DNA:

    deoxyribonucleic acid

    DNMT:

    DNA methyltransferases

    dNTP:

    deoxynucleoside triphosphate

    dsDNA:

    double stranded DNA

    dTTP:

    deoxythymidine triphosphate

    dUTP:

    deoxyuridine triphosphate

    EMC:

    enzyme mismatch cleavage

    FISH:

    fluorescence in-situ hybridization

    KCL:

    potassium chloride

    MAS:

    marker-assisted selection

    mRNA:

    messenger RNA

    MSP:

    methylation specific PCR

    NaOH:

    sodium hydroxide

    PAGE:

    polyacrilamide gel electrophoresis

    PCR:

    polymerase chain reaction

    PTT:

    protein truncation test

    qPCR:

    quantitative real time polymerase chain reaction

    RFLP:

    restriction fragment length polymorphism

    RNA:

    ribonucleic acid

    RT-PCR:

    reverse transcription polymerase chain reaction

    SDS-PAGE:

    sodium dodecyl sulfate polyacrilamide gel electrophoresis

    SSCP:

    single-stranded conformational polymorphism

    TGGE:

    temperature gradient gel electrophoresis

    tRNA:

    transfer RNA

    UV:

    ultra-violet

    VNTR:

    variable number tandem repeats

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    © 2009 Springer Science+Business Media, LLC

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    Castresana, J.S., Lázcoz, P. (2009). Techniques for DNA Analysis. In: Chedrese, P. (eds) Reproductive Endocrinology. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-88186-7_8

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    • DOI: https://doi.org/10.1007/978-0-387-88186-7_8

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    • Publisher Name: Springer, Boston, MA

    • Print ISBN: 978-0-387-88185-0

    • Online ISBN: 978-0-387-88186-7

    • eBook Packages: MedicineMedicine (R0)

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