Abstract
Current epiretinal implants contain a small number of electrodes with diameters of a few hundred microns. Smaller electrodes are desirable to increase the spatial resolution of artificial sight. To lay the foundation for the next generation of retinal prostheses, we assessed the stimulation efficacy of micro-fabricated arrays of 61 platinum disk electrodes with diameters 8–12 μm, spaced 60 μm apart. Isolated pieces of rat, guinea pig, and monkey retina were placed on the multi-electrode array ganglion cell side down and stimulated through individual electrodes with biphasic, charge-balanced current pulses. Spike responses from retinal ganglion cells were recorded either from the same or a neighboring electrode. Most pulses evoked only 1–2 spikes with short latencies (0.3–10 ms), and rarely was more than one recorded ganglion cell stimulated. Threshold charge densities for eliciting spikes in ganglion cells were typically below 0.15 mC/cm2 for pulse durations between 50 and 200 μs, corresponding to charge thresholds of ∼ 100 pC. Stimulation remained effective after several hours and at frequencies up to 100 Hz. Application of cadmium chloride did not abolish evoked spikes, implying direct activation. Thus, electrical stimulation of mammalian retina with small-diameter electrodes is achievable, providing high temporal and spatial precision with low charge densities.
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References
Humayun M (2003) Clinical trial results with a 16-electrode epiretinal implant in end-stage RP patients. In: The First DOE International Symposium on Artificial Sight. Fort Lauderdale, FL: Department of Energy.
Santos A, Humayun MS, de Juan E, Jr., Greenburg RJ, Marsh MJ, Klock IB, Milam AH (1997) Preservation of the inner retina in retinitis pigmentosa. A morphometric analysis. Arch Ophthalmol 115: 511–515.
Humayun MS, Prince M, de Juan E, Jr., Barron Y, Moskowitz M, Klock IB, Milam AH (1999) Morphometric analysis of the extramacular retina from postmortem eyes with retinitis pigmentosa. Invest Ophthalmol Vis Sci 40: 143–148.
Cha K, Horch KW, Normann RA (1992) Mobility performance with a pixelized vision system. Vision Res 32: 1367–1372.
Pollen DA (1977) Responses of single neurons to electrical stimulation of the surface of the visual cortex. Brain Behav Evol 14: 67–86.
Brummer SB, Robblee LS, Hambrecht FT (1983) Criteria for selecting electrodes for electrical stimulation: theoretical and practical considerations. Ann N Y Acad Sci 405: 159–171.
Tehovnik EJ (1996) Electrical stimulation of neural tissue to evoke behavioral responses. J Neurosci Methods 65: 1–17.
Humayun M, Propst R, de Juan E, Jr., McCormick K, Hickingbotham D (1994) Bipolar surface electrical stimulation of the vertebrate retina. Arch Ophthalmol 112: 110–116.
Weiland JD, Humayun MS, Dagnelie G, de Juan E, Jr., Greenberg RJ, Iliff NT (1999) Understanding the origin of visual percepts elicited by electrical stimulation of the human retina. Graefes Arch Clin Exp Ophthalmol 237: 1007–1013.
Suzuki S, Humayun MS, Weiland JD, Chen SJ, Margalit E, Piyathaisere DV, de Juan E, Jr. (2004) Comparison of electrical stimulation thresholds in normal and retinal degenerated mouse retina. Jpn J Ophthalmol 48: 345–349.
Dawson WW, Radtke ND (1977) The electrical stimulation of the retina by indwelling electrodes. Invest Ophthalmol Vis Sci 16: 249–252.
Wyatt J, Rizzo JF, Grumet A, Edell D, Jensen RJ (1994) Development of a silicon retinal implant: epiretinal stimulation of retinal ganglion cells in the rabbit. Invest Ophthalmol Vis Sci 35: 1380. ARVO abstract.
Rizzo JF, Grumet AE, Edell D, Wyatt J, Jensen R (1997) Single-unit recording following extracellular stimulation of retinal ganglion cell axons in rabbits. Invest Ophthalmol Vis Sci 38: S40.
Jensen RJ, Rizzo JF, 3rd, Ziv OR, Grumet A, Wyatt J (2003) Thresholds for activation of rabbit retinal ganglion cells with an ultrafine, extracellular microelectrode. Invest Ophthalmol Vis Sci 44: 3533–3543.
Hesse L, Schanze T, Wilms M, Eger M (2000) Implantation of retina stimulation electrodes and recording of electrical stimulation responses in the visual cortex of the cat. Graefes Arch Clin Exp Ophthalmol 238: 840–845.
Walter P, Heimann K (2000) Evoked cortical potentials after electrical stimulation of the inner retina in rabbits. Graefes Arch Clin Exp Ophthalmol 238: 315–318.
Humayun MS, Weiland JD, Fujii GY, Greenberg R, Williamson R, Little J, Mech B, Cimmarusti V, Van Boemel G, Dagnelie G, de Juan E (2003) Visual perception in a blind subject with a chronic microelectronic retinal prosthesis. Vision Res 43: 2573–2581.
Rizzo JF, 3rd, Wyatt J, Loewenstein J, Kelly S, Shire D (2003) Methods and perceptual thresholds for short-term electrical stimulation of human retina with microelectrode arrays. Invest Ophthalmol Vis Sci 44: 5355–5361.
Grumet A (1999) Electric stimulation parameters for an epi-retinal prosthesis. In: Department of Electrical Engineering and Computer Science, p 144: Massachusetts Institute Of Technology.
Grumet AE, Wyatt JL, Jr., Rizzo JF, 3rd (2000) Multi-electrode stimulation and recording in the isolated retina. J Neurosci Methods 101: 31–42.
Zrenner E, Stett A, Weiss S, Aramant RB, Guenther E, Kohler K, Miliczek KD, Seiler MJ, Haemmerle H (1999) Can subretinal microphotodiodes successfully replace degenerated photoreceptors? Vision Res 39: 2555–2567.
Stett A, Barth W, Weiss S, Haemmerle H, Zrenner E (2000) Electrical multisite stimulation of the isolated chicken retina. Vision Res 40: 1785–1795.
Meister M, Pine J, Baylor DA (1994) Multi-neuronal signals from the retina: acquisition and analysis. J Neurosci Methods 51: 95–106.
Chichilnisky EJ, Baylor DA (1999) Receptive-field microstructure of blue-yellow ganglion cells in primate retina. Nat Neurosci 2: 889–893.
Litke AM (1998) The retinal readout system: an application of microstrip detector technology to neurobiology. Nucl Instrum Methods Phys Res A 418: 203–209.
Litke AM, Chichilnisky EJ, Dabrowskic W, Grilloa AA, Grybosc P, S. K, Rahmand M, G. T (2003) Large-scale imaging of retinal output activity. Nucl Instrum Methods Phys Res A 501: 298–307.
Litke AM (1999) The retinal readout system: a status report. Nucl Instrum Methods Phys Res A 435: 242–249.
Brummer SB, Turner MJ (1977) Electrical stimulation with Pt electrodes: II-estimation of maximum surface redox (theoretical non-gassing) limits. IEEE Trans Biomed Eng 24: 440–443.
Wandell BA (1995) Foundations of Vision, Sunderland, MA: Sinauer.
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Sekirnjak, C., Hottowy, P., Sher, A., Dabrowski, W., Litke, A.M., Chichilnisky, E. (2007). Electrical Stimulation of Mammalian Retinal Ganglion Cells Using Dense Arrays of Small-Diameter Electrodes. In: Humayun, M.S., Weiland, J.D., Chader, G., Greenbaum, E. (eds) Artificial Sight. Biological and Medical Physics, Biomedical Engineering. Springer, New York, NY. https://doi.org/10.1007/978-0-387-49331-2_18
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