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Application of Spectroscopic and Calorimetric Techniques in Protein Formulation Development

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Misbehaving Proteins

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Abstract

The rational development of stable protein formulations requires a detailed understanding of the factors influencing the different routes of protein degradation. Aggregation, one of the major routes of protein degradation, is dependent on, among other factors, the conformational stability of the molecule. Assessing the conformational structure and the factors that affect it, usually via spectroscopic and calorimetric methods, is increasingly used in formulation development to better understand the influence of formulation variables on protein folding and/or aggregation.

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Authors
  1. Angela Wilcox
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  2. Rajesh Krishnamurthy
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Wilcox, A., Krishnamurthy, R. (2006). Application of Spectroscopic and Calorimetric Techniques in Protein Formulation Development. In: Misbehaving Proteins. Springer, New York, NY. https://doi.org/10.1007/978-0-387-36063-8_5

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