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Large Scale Production of Stem Cells and Their Derivatives

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Engineering of Stem Cells

Part of the book series: Advances in Biochemical Engineering / Biotechnology ((ABE,volume 114))

Abstract

Stem cells have been envisioned to become an unlimited cell source for regenerative medicine. Notably, the interest in stem cells lies beyond direct therapeutic applications. They might also provide a previously unavailable source of valuable human cell types for screening platforms, which might facilitate the development of more efficient and safer drugs. The heterogeneity of stem cell types as well as the numerous areas of application suggests that differential processes are mandatory for their in vitro culture. Many of the envisioned applications would require the production of a high number of stem cells and their derivatives in scalable, well-defined and potentially clinical compliant manner under current good manufacturing practice (cGMP). In this review we provide an overview on recent strategies to develop bioprocesses for the expansion, differentiation and enrichment of stem cells and their progenies, presenting examples for adult and embryonic stem cells alike.

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Abbreviations

(NOD/SCID):

miceNonobese diabetic/severe combined immunodeficient

(RWV):

bioreactorRotating wall vessel

bFGF, FGF-2:

Basic fibroblast growth factor

BM:

Bone marrow

BMP:

Bone morphogenetic protein

BMP:

Morphogenetic protein

cGMP:

Current good manufacturing practice

CHO:

Hamster ovary cells

EBs:

Embryoid bodies

ESC:

Embryonic stem cells

GM-CSF:

Granulocyte macrophage colony stimulating factor

hESC:

Human embryonic stem cell

hNPC:

Neural precursor cells

HSC:

Hematopoietic stem and progenitor cells

LIF:

Leukemia inhibitory factor bone

MASC:

Magnetic activated cell sorting

MI:

Myocardial infarction

MSC:

Mesenchymal stem cells

NSC:

Neural stem cells

PB:

(Mobilized) Peripheral blood

SCF:

Stem cell factor

SNM`:

Spherical neural masses

TGF-beta:

Transforming growth factor beta

UCB:

Umbilical cord blood

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Acknowledgements

I thank Blaine Phillips, William Rust, Birgit Andree, Harmeet Singh, Zhou WeiZhuang (Institute of Medical Biology, Singapore) and Andre Choo (Bioprocessing Technology Institute, Singapore) for helpful comments and a critical review of this manuscript.

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  1. Institute of Medical Biology (IMB), 8A Biomedical Grove, # 06-06 Immunos, Level 5, Room # 5.04, Singapore, 138648, Singapore

    Robert Zweigerdt

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  1. Leibnitz Res.Lab.Biotechnol., Hannover Medical School, Podbielskistr. 380, Hannover, 30659, Germany

    Ulrich Martin

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Zweigerdt, R. (2009). Large Scale Production of Stem Cells and Their Derivatives. In: Martin, U. (eds) Engineering of Stem Cells. Advances in Biochemical Engineering / Biotechnology, vol 114. Springer, Berlin, Heidelberg. https://doi.org/10.1007/10_2008_27

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