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Membrane skeleton detachment in spherical and cylindrical microexovesicles

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Abstract

We observed that amphiphile-induced microexovesicles may be spherical or cylindrical, depending on the species of the added amphiphile. The spherical microexovesicle corresponds to an extreme local difference between the two monolayer areas of the membrane segment with a fixed area, while the cylindrical microexovesicle corresponds to an extreme local area difference if the area of the budding segment is increased due to lateral influx of anisotropic membrane constituents. Protein analysis showed that both types of vesicles are highly depleted in the membrane skeleton. It is suggested that a partial detachment of the skeleton in the budding region is favoured due to accumulated skeleton shear deformations in this region.

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Authors and Affiliations

  1. Department of Biology, Åbo Akademi University, FIN-20520, Åbo/Turku, Finland

    Henry Hägerstrand & Malgorzata Bobrowska-Hägerstrand

  2. Institute of Biophysics, Medical Faculty, University of Ljubljana, SI-1000, Ljubljana, Slovenia

    Veronika Kralj-Iglič

  3. Laboratory of Applied Physics, Faculty of Electrical Engineering, University of Ljubljana, SI-1000, Ljubljana, Slovenia

    Aleš Iglič

  4. Faculty of Electrical Engineering, Tržaška 25, SI-1000, Ljubljana, Slovenia

    Aleš Iglič

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  1. Henry Hägerstrand
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  2. Veronika Kralj-Iglič
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  3. Malgorzata Bobrowska-Hägerstrand
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  4. Aleš Iglič
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Correspondence to Aleš Iglič.

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Hägerstrand, H., Kralj-Iglič, V., Bobrowska-Hägerstrand, M. et al. Membrane skeleton detachment in spherical and cylindrical microexovesicles. Bull. Math. Biol. 61, 1019–1030 (1999). https://doi.org/10.1006/bulm.1999.0128

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  • DOI: https://doi.org/10.1006/bulm.1999.0128

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