Abstract
Dissolved organic matter (DOM) in water from two lakes (spring and autumn samples) was isolated and fractionated by ultrafiltration (UF) and XAD-8 techniques. The difference between the spring and autumn samples was greatest for the colour, CODMn and DOC values. High-performance size-exclusion chromatography gave useful information on the molecular size distribution of the DOM. The two largest fractions of DOM were within the nominal molecular mass cutoffs 103–104 and 104–105, respectively. Decreasing of DOM of UF-concentrates in the XAD-8 treatment was the greatest for the former cutoff. Some similarity of molecular masses (weight and number averages) could be found between the DOM-fractions within the 103–104 cutoff isolated by UF and fulvic acids isolated directly from the original water samples by the XAD-8 technique. The total amounts of humic substances separated from the UF-concentrates were about 25% higher than those obtained directly from the original water samples. The results show that the usefulness of a given reference humus depends strongly on its origin.
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Peuravuori, J., Pihlaja, K. (1991). Isolation and fractionation of humic substances in lake waters. In: Allard, B., Borén, H., Grimvall, A. (eds) Humic Substances in the Aquatic and Terrestrial Environment. Lecture Notes in Earth Sciences, vol 33. Springer, Berlin, Heidelberg. https://doi.org/10.1007/BFb0010464
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DOI: https://doi.org/10.1007/BFb0010464
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