Chapter

1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies

Volume 53 of the series IFMBE Proceedings pp 219-222

Antimetastatic Potential in Mice after Gene Therapy with Plasmid AMEP

  • M. BosnjakAffiliated withInstitute of Oncology Ljubljana, Department of Experimental Oncology
  • , U. KamensekAffiliated withInstitute of Oncology Ljubljana, Department of Experimental Oncology
  • , A. SedlarAffiliated withInstitute of Oncology Ljubljana, Department of Experimental Oncology
  • , M. CemazarAffiliated withInstitute of Oncology Ljubljana, Department of Experimental Oncology
  • , J. ZavrsnikAffiliated withDepartment of Biochemistry and Molecular and Structural Biology, Jozef Stefan Institute
  • , B. TurkAffiliated withDepartment of Biochemistry and Molecular and Structural Biology, Jozef Stefan Institute
  • , Celine BouquetAffiliated withONXEO, Boulevard du Général Martial Valin 49
  • , G. SersaAffiliated withInstitute of Oncology Ljubljana, Department of Experimental Oncology

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Abstract

Gene electrotransfer of Plasmid AMEP is a new antiangiogenic approach in cancer treatment. While the local effects after intratumoral administration have been already studied, systemic effects still need to be elucidated. Therefore intramtumoral or intramuscular gene therapy with Plasmid AMEP was performed and effects on distant tumors or metastases were observed and compared. Only 8% of the mice developed distant metastases after Plasmid AMEP gene electrotransfer, compared to 40% in the control groups. Additionally, intramuscular gene elctrotransfer performed before the tumor implantation, has no effect on the tumor outgrowth regardless of the GET protocol used. Study showed that gene therapy with Plasmid AMEP has antimetastatic potential only after intratumoral, but not after intramuscular gene electrotransfer. Most probably circulating AMEP peptide produced in muscle cells may be rapidly biodegraded and could not be able to reach tumor site in high amounts needed for AMEP effectiveness.

Keywords

gene electrotransfer Plasmid AMEP antimetastatic potential melanoma