Skip to main content

Improving “Shrinkage-Swelling” Response of Expansive Soil Using Bio-calcite and Exopolysaccharide Produced by Bacillus sp.

  • Conference paper
  • First Online:
Advances in Computer Methods and Geomechanics

Part of the book series: Lecture Notes in Civil Engineering ((LNCE,volume 56))

  • 797 Accesses

Abstract

Biological phenomena standout as a key towards green method for improving the properties of engineering construction material. The present study investigates the effect of Microbial Induced Calcite Precipitation (MICP) and Extracellular Polymeric Substance (EPS) produced by Bacillus cereus (B. cereus) SG4 on “shrinkage-swelling” behavior of expansive soil. The soil used for the study was commercially available Bentonite cohesive soil. The critical soil parameters such as Liquid Limit (LL), Plastic Limit (PL), and Differential Free Swell Index (DFSI) were found to be very high (LL = 608%, PL = 50%, and DFSI = 661%) due to the presence of Montmorillonite mineral. The results showed that treatment of Bentonite expansive soil with bio-calcite and EPS containing B. cereus SG4 culture media worked effectively. Bentonite soil was treated with bacteria along with culture medium for 5 and 10 days. It was observed that there was no significant reduction in geotechnical properties after 10th day of treatment. Maximum effect was observed at the end of 5th day exhibiting the efficiency and strong capability of proposed soil treatment method. After 5th day, LL, PL, and DFSI values were observed to be decreased; 177%, 39%, and 371% for EPS, respectively. The similar response was observed for Bio-calcite technique, which exhibited a significant reduction in LL, PL, and DFSI values (158%, 39%, and 271%), respectively. Both the treatment techniques worked successfully in improving the shrinkage-swelling response of Bentonite soil, but bio-calcite treatment was observed to be more effective than EPS treatment to control the shrinkage-swelling response.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 259.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Achal V, Mukherjee A, Reddy MS (2011) Effect of calcifying bacteria on permeation properties of concrete structures. J Ind Microbiol Biotechnol 38:1229–1234

    Article  Google Scholar 

  2. Anbu P, Kang C-H, Shin Y-J, So J-S (2016) Formations of calcium carbonate minerals by bacteria and its multiple applications. Springerplus 5:250

    Article  Google Scholar 

  3. Antón J, Meseguer I, Rodríguez-Valera F (1988) Production of an extracellular polysaccharide by Haloferax mediterranei. Appl Environ Microbiol 54:2381–2386

    Article  Google Scholar 

  4. Bachmeier KL, Williams AE, Warmington JR, Bang SS (2002) Urease activity in microbiologically-induced calcite precipitation. J Biotechnol 93:171–181

    Article  Google Scholar 

  5. Banagan BL, Wertheim BM, Roth MJS, Caslake LF (2010) Microbial strengthening of loose sand. Lett Appl Microbiol 51:138–142

    Google Scholar 

  6. Bell FG (1996) Lime stabilization of clay minerals and soils. Eng Geol 42:223–237

    Article  Google Scholar 

  7. Ceyhan N, Ozdemir G (2008) Extracellular polysaccharides produced by cooling water tower biofilm bacteria and their possible degradation. Biofouling 24:129–135

    Article  Google Scholar 

  8. Cui Y-J, Tang A-M, Qian L-X et al (2011) Thermal-mechanical behavior of compacted GMZ bentonite. Soils Found 51:1065–1074

    Article  Google Scholar 

  9. Daniels J, Cherukuri R (2005) Influence of biofilm on barrier material performance. Pract Period Hazard Toxic Radioact Waste Manag 9:245–252

    Article  Google Scholar 

  10. Dash S, Hussain M (2011) Lime stabilization of soils: reappraisal. J Mater Civ Eng 24:707–714

    Article  Google Scholar 

  11. De Muynck W, Debrouwer D, De Belie N, Verstraete W (2008) Bacterial carbonate precipitation improves the durability of cementitious materials. Cem Concr Res 38:1005–1014

    Article  Google Scholar 

  12. DeJong JT, Mortensen BM, Martinez BC, Nelson DC (2010) Bio-mediated soil improvement. Ecol Eng 36:197–210

    Article  Google Scholar 

  13. DuBois M, Gilles KA, Hamilton JK et al (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356

    Article  Google Scholar 

  14. Flemming H-C, Wingender J (2010) The biofilm matrix. Nat Rev Micro 8:623–633

    Article  Google Scholar 

  15. Frank JA, Reich CI, Sharma S et al (2008) Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA Genes. Appl Environ Microbiol 74:2461–2470

    Article  Google Scholar 

  16. Ghosh S, Biswas M, Chattopadhyay BD, Mandal S (2009) Microbial activity on the microstructure of bacteria modified mortar. Cem Concr Compos 31:93–98

    Article  Google Scholar 

  17. Ivanov V, Chu J (2008) Applications of microorganisms to geotechnical engineering for bioclogging and biocementation of soil in situ. Rev Environ Sci Bio/Technol 7:139–153

    Article  Google Scholar 

  18. Jahns T, Zobel A, Kleiner D, Kaltwasser H (1988) Evidence for carrier-mediated, energy-dependent uptake of urea in some bacteria. Arch Microbiol 149:377–383

    Article  Google Scholar 

  19. Krishnapriya S, Venkatesh Babu DL, Pa G (2015) Isolation and identification of bacteria to improve the strength of concrete. Microbiol Res 174:48–55

    Article  Google Scholar 

  20. Larkin MA, Blackshields G, Brown NP et al (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23:2947–2948

    Article  Google Scholar 

  21. Lian B, Hu Q, Chen J et al (2006) Carbonate biomineralization induced by soil bacterium Bacillus megaterium. Geochim Cosmochim Acta 70:5522–5535

    Article  Google Scholar 

  22. Mortensen BM, Haber MJ, DeJong JT et al (2011) Effects of environmental factors on microbial induced calcium carbonate precipitation. J Appl Microbiol 111:338–349

    Article  Google Scholar 

  23. Natarajan KR (1995) Kinetic study of the enzyme urease from Dolichos biflorus. J Chem Educ 72:556

    Article  Google Scholar 

  24. Paul F, Morin A, Monsan P (1986) Microbial polysaccharides with actual potential industrial applications. Biotechnol Adv 4:245–259

    Article  Google Scholar 

  25. Pei R, Liu J, Wang S, Yang M (2013) Use of bacterial cell walls to improve the mechanical performance of concrete. Cem Concr Compos 39:122–130

    Article  Google Scholar 

  26. Rittmann BE, Crawford L, Tuck CK, Namkung E (1986) In situ determination of kinetic parameters for biofilms: isolation and characterization of oligotrophic biofilms. Biotechnol Bioeng 28:1753–1760

    Article  Google Scholar 

  27. Sarda D, Choonia HS, Sarode DD, Lele SS (2009) Biocalcification by Bacillus pasteurii urease: a novel application. J Ind Microbiol Biotechnol 36(8):1111–1115

    Article  Google Scholar 

  28. Siddique R, Chahal NK (2011) Effect of ureolytic bacteria on concrete properties. Constr Build Mater 25:3791–3801

    Article  Google Scholar 

  29. Soon NW, Lee LM, Khun TC, Ling HS (2013) Improvements in engineering properties of soils through microbial-induced calcite precipitation. KSCE J Civ Eng 17:718–728

    Article  Google Scholar 

  30. Tamura K, Stecher G, Peterson D et al (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  Google Scholar 

  31. Tsuru D, Fukumoto J, Yamamoto T (1974) Process for producing detergent resisting alkaline protease. U.S. Patent No. 3,838,009

    Google Scholar 

  32. Uppal HL, Chadda LR (1967) Physico-chemical changes in the lime stabilization of black cotton soil (India). Eng Geol 2:179–189

    Article  Google Scholar 

  33. Wang JY, Soens H, Verstraete W, De Belie N (2014) Self-healing concrete by use of microencapsulated bacterial spores. Cem Concr Res 56:139–152

    Article  Google Scholar 

  34. Wingender J, Neu TR, Flemming H-C (1999) What are bacterial extracellular polymeric substances? In: Wingender J, Neu TR, Flemming H-C (eds) Microbial extracellular polymeric substances: characterization, structure and function. Springer, Berlin, Heidelberg, pp 1–19

    Chapter  Google Scholar 

  35. Zhu T, Dittrich M (2016) Carbonate precipitation through microbial activities in natural environment, and their potential in biotechnology: a review. Front Bioeng Biotechnol 4:4. https://doi.org/10.3389/fbioe.2016.00004

    Article  Google Scholar 

Download references

Acknowledgements

Authors thank Indian Institute of Technology Gandhinagar for providing financial support for this work. Authors also thank Ms. Gundeep Kaur Sudan, Ms. S. Smita for their assistance in bacterial screening and Mr. R. Vijayaraghavan (NIOT, Chennai) for his help with genomic characterization and analysis. Authors are very grateful to Dr. Abhijit Mukherjee for valuable discussions and Dr. V. Veeraraghavan for proofreading the manuscript.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Kaling Taki .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2020 Springer Nature Singapore Pte Ltd.

About this paper

Check for updates. Verify currency and authenticity via CrossMark

Cite this paper

Krishna Kumar, V.G., Taki, K., Gupta, S., Sachan, A. (2020). Improving “Shrinkage-Swelling” Response of Expansive Soil Using Bio-calcite and Exopolysaccharide Produced by Bacillus sp.. In: Prashant, A., Sachan, A., Desai, C. (eds) Advances in Computer Methods and Geomechanics . Lecture Notes in Civil Engineering, vol 56. Springer, Singapore. https://doi.org/10.1007/978-981-15-0890-5_8

Download citation

  • DOI: https://doi.org/10.1007/978-981-15-0890-5_8

  • Published:

  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-15-0889-9

  • Online ISBN: 978-981-15-0890-5

  • eBook Packages: EngineeringEngineering (R0)

Publish with us

Policies and ethics