Abstract
When applying a new material as a bioink in microextrusion bioprinting, numerous considerations need to be addressed. As stated in Chap. 3, one of the primary considerations is printability, which normally encompasses the material’s ability to (i) be injected from a printhead (ii) undergo rapid gelation upon deposition, and (iii) exhibit suitable mechanical properties that would support the printed structure. Given this, we seek to explore hydrogel formulation that could meet these criteria. Specifically, in this chapter, we will apply a shear-thinning and rapidly self-healing guest–host hydrogel based on hyaluronic acid (HA) to microextrusion 3DP. Adamantane (Ad, guest) and β-cyclodextrin (CD, host) moieties are separately coupled to HA, to create two hydrogel precursors that form a supramolecular assembly upon mixing [1]. It is hypothesized that such formulation would allow for smooth extrusion and temporary stabilization post-extrusion because of the shear-thinning and self-healing properties, respectively. To enhance the structural integrity of the supramolecularly crosslinked hydrogel, we also introduce photo-crosslinkable groups onto the macromers. We then investigate how each type of crosslinking (guest–host, photo-crosslinking, and their combination) affects the printability of multilayer scaffolds. Post-crosslinking methods are also explored concerning structural integrity and stability over time. Printed structures can be further functionalized to support cell culture. Similar dual-crosslinking mechanisms based on supramolecular and covalent bonding may enable the development of 3D printable hydrogel bioinks from materials that cannot otherwise be printed.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Rodell CB, Kaminski AL, Burdick JA (2013) Rational design of network properties in guest-host assembled and shear-thinning hyaluronic acid hydrogels. Biomacromolecules 14(11):4125–4134
Ouyang L, Highley CB, Rodell CB, Sun W, Burdick JA (2016) 3D printing of shear-thinning hyaluronic acid hydrogels with secondary cross-linking. ACS Biomater Sci Eng 2(10):1743–1751
Burdick JA, Prestwich GD (2011) Hyaluronic acid hydrogels for biomedical applications. Adv Mater 23(12):H41–H56
Highley CB, Prestwich GD, Burdick JA (2016) Recent advances in hyaluronic acid hydrogels for biomedical applications. Curr Opin Biotechnol 40:35–40
Rodell CB, MacArthur JW, Dorsey SM, Wade RJ, Wang LL, Woo YJ, Burdick JA (2015) Shear-thinning supramolecular hydrogels with secondary autonomous covalent crosslinking to modulate viscoelastic properties in vivo. Adv Func Mater 25(4):636–644
Bian L, Guvendiren M, Mauck RL, Burdick JA (2013) Hydrogels that mimic developmentally relevant matrix and N-cadherin interactions enhance MSC chondrogenesis. Proc Natl Acad Sci U S A 110(25):10117–10122
Gramlich WM, Kim IL, Burdick JA (2013) Synthesis and orthogonal photopatterning of hyaluronic acid hydrogels with thiol-norbornene chemistry. Biomaterials 34(38):9803–9811
Lim KS, Schon BS, Mekhileri NV, Brown GCJ, Chia CM, Prabakar S, Hooper GJ, Woodfield TBF (2016) New visible-light photoinitiating system for improved print fidelity in gelatin-based bioinks. ACS Biomater Sci Eng 2(10):1752–1762
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Copyright information
© 2019 Tsinghua University Press, Beijing and Springer Nature Singapore Pte Ltd.
About this chapter
Cite this chapter
Ouyang, L. (2019). 3D Bioprinting of Shear-Thinning Self-assembly Bioink. In: Study on Microextrusion-based 3D Bioprinting and Bioink Crosslinking Mechanisms. Springer Theses. Springer, Singapore. https://doi.org/10.1007/978-981-13-9455-3_4
Download citation
DOI: https://doi.org/10.1007/978-981-13-9455-3_4
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-13-9454-6
Online ISBN: 978-981-13-9455-3
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)