Abstract
Toxins from the posterior salivary gland (PSG) of cuttlefish are known toxins with pronounced toxicity. In this chapter, crude toxins from Sepia pharashadi are fractionated by ion-exchange chromatography and purified by reversed-phase high-performance liquid chromatography (RP-HPLC). The yield protein and carbohydrate contents of the PSG toxin are estimated to be 1.61 mg/g and 0.06 mg/g, respectively. Fourier transform infrared spectroscopy (FT-IR) of PSG toxin affirmed the incidence of CO-NH, CH and conjugated alkyl, alcoholic OH and primary NH functional groups. Circular dichroism (CD) spectroscopy and K2D analysis of the PSG toxin authenticated the attendance of secondary structure with 37% α-helix, 26% β sheet and 38% random coil. Teratogenicity of PSG toxin against Zebrafish embryo exhibited evolving malformations and premature hatching at a maximum tolerated dose of 1.0 μM. These findings strongly exhibit the toxicity of the ionic peptide-rich PSG toxin from S. pharashadi and its utilisation for its promise as a prospective cytotoxic agent of the future.
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Acknowledgement
The author gratefully acknowledges the Department of Biotechnology, Ministry of Science and Technology, Government of India (BT/PR15676/AAQ/03/794/2016).
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Ramachandran, S., Rajagopal, S. (2019). Teratogenic Activity of Toxins in Zebrafish Model. In: Zebrafish: A Model for Marine Peptide Based Drug Screening. Springer, Singapore. https://doi.org/10.1007/978-981-13-7844-7_3
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DOI: https://doi.org/10.1007/978-981-13-7844-7_3
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