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Molecular Diagnosis of Enteric Bacterial Pathogens

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DNA Fingerprinting: Advancements and Future Endeavors

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Abstract

Bacterial strains belonging to family Enterobacteriaceae are well-established enteric pathogens of humans and animals which are the major cause of mortality worldwide. So to deal with these infections, it is necessary to identify the disease-causing pathogens along with their virulence mechanism. The characterization and detection of these pathogens rely on conventional culturing and biochemical techniques. But these conventional techniques are low sensitive and time intense. This led to search for more rapid, sensitive, and advanced technique for their detection. In the present scenario, the molecular methods are most commonly applied to identify the pathogenic bacterial strains. The molecular methods include DNA-based methods, such as restriction endonuclease analysis of genomic and plasmid DNA, plasmid profiling, chromosomal DNA profiling using pulsed field gel electrophoresis, polymerase chain reaction (PCR)-based methods, 16S rRNA sequencing methods, polyphasic taxonomic approaches, etc. These are the more rapid and more sensitive techniques which overcome the issues of identification by conventional techniques. Hence, these techniques are considered the most promising for bacterial identification.

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Author information

Authors and Affiliations

  1. Department of P. G. Studies and Research in Biological Sciences, Rani Durgavati University, Jabalpur, Madhya Pradesh, India

    Amita Shrivastava & Pradeep K. Singhal

  2. DNA Fingerprinting Unit, State Forensic Science Laboratory, Sagar, Madhya Pradesh, India

    Pankaj Shrivastava

Authors
  1. Amita Shrivastava
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  2. Pradeep K. Singhal
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  3. Pankaj Shrivastava
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Editors and Affiliations

  1. DNA Fingerprinting Unit, State Forensic Science Laboratory, Sagar, Madhya Pradesh, India

    Hirak Ranjan Dash

  2. DNA Fingerprinting Unit, State Forensic Science Laboratory, Sagar, Madhya Pradesh, India

    Pankaj Shrivastava

  3. Department of Biology and DNA Fingerprinting Unit, Central Forensic Science Laboratory, New Delhi, Delhi, India

    Braja Kishore Mohapatra

  4. Department of Life Science, National Institute of Technology, Rourkela, Odisha, India

    Surajit Das

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Shrivastava, A., Singhal, P.K., Shrivastava, P. (2018). Molecular Diagnosis of Enteric Bacterial Pathogens. In: Dash, H., Shrivastava, P., Mohapatra, B., Das, S. (eds) DNA Fingerprinting: Advancements and Future Endeavors. Springer, Singapore. https://doi.org/10.1007/978-981-13-1583-1_9

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