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G protein-coupled receptor expression in Halobacterium salinarum

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Perspectives on Solid State NMR in Biology

Part of the book series: Focus on Structural Biology ((FOSB,volume 1))

Abstract

A fundamental problem in the study of transmembrane proteins is the availability of protein, in the quantities required for detailed biochemical and biophysical characterization [see reviews 1,2]. Atomic resolution structures have solved for a handful of membrane proteins, including photoreaction centers [3–6], prostaglandin H2 synthase [7], porins of various species [8,9], cytochrome c oxidase [10], bacteriorhodopsin [11,12] a potassium channel [13], and bovine rhodopsin [14]. The structural characterization of these proteins was achieved, in part, because they were naturally available at very high expression levels.

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Winter-Vann, A.M., Martinez, L., Bartus, C., Levay, A., Turner, G.J. (2001). G protein-coupled receptor expression in Halobacterium salinarum . In: Kiihne, S.R., de Groot, H.J.M. (eds) Perspectives on Solid State NMR in Biology. Focus on Structural Biology, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2579-8_13

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  • DOI: https://doi.org/10.1007/978-94-017-2579-8_13

  • Publisher Name: Springer, Dordrecht

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