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Diagnosis and Identification of IPNV in Salmonids by Molecular Methods

  • Chapter
Molecular Diagnosis of Salmonid Diseases

Part of the book series: Reviews: Methods and Technologies in Fish Biology and Fisheries ((REME,volume 3))

Abstract

Fifty years ago, Infectious Pancreatic Necrosis Virus (IPNV) was the first virus to be isolated from fish, and hence the origin of the development of fish virology.

After the initial studies in which farmed salmonids were considered the unique host, the virus was also found in a large number of species of freshwater and marine fishes as well as in crustaceans and molluscs. The economic impact of the disease and the ubiquity of the agent justifies the extensive literature related to the IPN virus including numerous reviews.

At present, the complete genomic sequence of the dsRNA has been published for four different strains belonging to three of the serotypes described. Based on these findings, a number of molecular procedures have been developed not only to characterize the virus but also to improve the diagnostic methods, trying to circumvent the necessity for cell cultures in order to detect IPNV directly in infected fish and ova.

The present review is focused on the molecular procedures used in the study of IPNV, using methods such as analysis of electropherotypes, RFLPs, nucleic acid hybridization using specific probes, and different RT-PCR protocols. The distinct procedures for extraction of viral RNA, selection of restriction enzymes, sets of primers or probes, as well as the conditions (concentration of virus, minimal time required) to obtain reliable results are examined.

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References

  • Adair, B.M. and Ferguson, H.W. (1981) Isolation of infectious pancreatic necrosis (IPN) virus from nonsalmonid fish. J. Fish. Dis. 4, 69–76.

    Article  Google Scholar 

  • Ahne, W. (1978) Isolation and characterization of infectious pancreatic necrosis virus from pike (Esox lucius). Arch. Virol. 58, 65–69.

    Article  PubMed  CAS  Google Scholar 

  • Ahne, W., Kohlmeyer, G. and Thomsen, I. (1986) The prevalence of infectious pancreatic necrosis virus antibodies in rainbow trout in the Federal Republic of Germany. Tieraerztl. Umsch. 41, 541–547.

    Google Scholar 

  • Ahne, W. and Thomsen, I. (1986) Infectious pancreatic necrosis: detection of virus and antibodies in rainbow trout IPNV-carrier (Salmo gairdneri). Zentralbi. Veterinarmed. B. 33, 552–554.

    CAS  Google Scholar 

  • Alonso, M., Rodriguez, S. and Perez-Prieto, S.I. (1999) Viral coinfection in salmonids: infectious pancreatic necrosis virus interferes with infectious hematopoietic necrosis virus. Arch. Virol. 144, 657–73.

    Article  PubMed  CAS  Google Scholar 

  • Baga, J., Toranzo, A.E., Lemos, M. and Hetrick, F.M. (1983) Influence of water temperature and salinity on the survival of IPN and IHN viruses. Bull. Eur. Ass. Fish Pathol. 3, 47–50

    Google Scholar 

  • Batts, W.N. and Winton, J.R. (1994) Optimizing the use of DNA probes for fish viruses. Int. Symp. Aquat. An. Health. Sept. 4–8, Seattle, Washington. pp. W–12. 3.

    Google Scholar 

  • Berthe, F.C.J., Burreson, E.M. and Hine, M. (1999) Use of molecular tools for molluscs disease diagnosis. Bull Eur. Ass. Fish Pathol. 19, 277–278.

    Google Scholar 

  • Biering, E. and Bergh, O. (1996) Experimental infection of Atlantic halibut Hippoglossus hippoglossus L., yolk-sac larvae with infectious pancreatic necrosis virus: Detection of virus by immunohistochemistry and in situ hybridization. J. Fish Dis. 19, 261–269.

    Article  Google Scholar 

  • Biering, E., Melby, H. and Mortensen, S. (1997) Sera and genotyping of some marine aquatic birnavirus isolates from Norway. Dis. Aquat. Org. 28, 169–174.

    Google Scholar 

  • Blake, S.L., Schill, W.B., McAllister, P.E., Lee, M.K., Singer, J.T. and Nicholson, B.L. (1995) Detection and identification of aquatic birnaviruses by PCR assay. J. Clin. Microbial. 33, 835–839.

    CAS  Google Scholar 

  • Bonami, J.R. and Adams, J.R. (1991) Birnaviridae. In: Adams J.R. and Bonami J.R. eds. Atlas of invertebrate viruses. CRC Press. p. 435–442.

    Google Scholar 

  • Brocchi, E., Comuzzi, M., Alborali, L. and Bovo, G. (1996) Monoclonal antibodies against the Sp serotype of infectious pancreatic necrosis virus (IPNV). (Italian). Bull. Soc. Ital. Pato. Ittica. 9, 40–51.

    Google Scholar 

  • Bullock, G., Rucker. R., Amend, D., Wolf, K. and Stucney H. (1976) Infectious pancreatic necrosis: transmission with iodine-treated and nontreated eggs of brook trout (Salvelinus fontinalis) J.Fish. Res. Board Can. 33, 1197–1198.

    Google Scholar 

  • Byrne, B.C., Li, J.J., Sninsky, L. And Poiesz, B.J. (1988) Detection of HIV-1 RNA sequences by in vitro DNA amplification. Nucleic Acids Res. 16, 41–65.

    Google Scholar 

  • Castric, J., Baudin-Laurencin, F., Coustans, M.F. and Auffret, M. (1987) Isolation of infectious pancreatic necrosis virus, Ab serotype, from an epizootic in farmed turbot, Scophthalmus maximus. Aquaculture (Special Issue: PAMAQ II). 67, 117–126.

    Google Scholar 

  • Caswell-Reno, P., Reno, P.W. and Nicholson, B.L. (1986) Monoclonal antibodies to infectious pancreatic necrosis virus: analysis of viral epitopes and comparison of different isolates. J. Gen. Virol. 67, 2193–2205.

    Google Scholar 

  • Chang, N., Macdonald, R.D. and Yamamoto, T. (1978) Purification of infectious pancreatic necrosis (IPN) virus and comparison of polypeptide composition of different isolates. Can. J. Microbiol. 24, 1927.

    Article  Google Scholar 

  • Chen, S.N., Shih, H.H. and Kou, G.H. (1985) The enzyme-linked immunosorbent assay (ELISA) for the detection of infectious pancreatic necrosis virus (IPNV) and eel virus european (EVE) in tissue culture and infected fish. COA Fish. Ser. No. 4. Fish Dis. Res. VII: 1–8.

    Google Scholar 

  • Chi, S.C., Chen, S.N. and Kou, G.H. (1991) Establishment, characterization and application of monoclonal antibodies against eel virus European (EVE). Fish Pathol. 26, 1–8.

    Article  CAS  Google Scholar 

  • Chomczynski, P. and Sachi, N. (1987) Single step method of RNA isolation by acid guanidinium thiocyanate-phenol chloroform extraction. Anal. Biochem. 162, 156–159.

    Article  PubMed  CAS  Google Scholar 

  • Chung, H.K., Lee, S.H., Lee, H.H., Ha, Y.L., Lee, D.S. and Kim, Y.S. (1993) Nucleotide sequence analysis of the VP2-NS-VP3 genes of infectious pancreatic necrosis virus DRT strain. Mol. Cells. 4, 341–354.

    Google Scholar 

  • Chung, H.K., Lee, S.H., Soo-Young, K., and Lee, H.H. (1994) Nucleotide sequence analysis of the RNA-dependent RNA polymerase gene of infectious pancreatic necrosis virus DRT strain. J. Microbiol. Biotechnol. 4, 264–269.

    CAS  Google Scholar 

  • Christie, K.E., Havarstein, L.S., Djupvik, H.O., Ness, S. and Endresen, C. (1988) Characterization of a new serotype of infectious pancreatic necrosis virus isolated from Atlantic salmon. Arch. Virol. 103, 167–177.

    Article  PubMed  CAS  Google Scholar 

  • Christie, K.E., Ness, S. and Djupvik, H.O. (1990) Infectious pancreatic necrosis virus in Norway: Partial serotyping by monoclonal antibodies, J. Fish Dis. 13, 323–327.

    Article  Google Scholar 

  • Clewely, J.P. (1989) The polymerase chain reaction, a review of the practical limitations for human immunodeficiency virus diagnosis. J. Virol. Methods. 25, 179–187.

    Article  Google Scholar 

  • Comps, M., Mari, J., Poisson, F. And Bonami, J.R. 1991. Biophysical and biochemical properties of an unusual bimavirus pathogenic for rotifers. J. Gen. Virol. 72, 1229–1236.

    CAS  Google Scholar 

  • Crane, M.St.J., Hardy-Smith, P., Williams, L.M., Hyatt, A.D., Eaton, L.M., Gould, A., Handlinger, J., Kattenbelt, J. and Gudkovs, N. (2000) First isolation of an aquatic birnavirus from farmed and wild fish species in Australia. Dis. Aquat. Org. 43, 1–14.

    Google Scholar 

  • Cutrin, J.M. (1998) Molecular analysis of bimavirus from molluscs and fish: Role of reservoirs in the spreading of the virus. Doctoral dissertation. University of Santiago de Compostela, Spain. (in spanish)

    Google Scholar 

  • Cutrin, J.M., Olveira, J.G., Bandín, I., Toranzo, A.E. and Dopazo, C.P. (1997) Use of RFLPs to determine the role of molluscs and crustaceans in the spreading of IPN viruses. EAFP VIIIth Int. Conf. “Diseases of Fish and Shellfish”. Edinburgh. 0. 65

    Google Scholar 

  • Cutrin, J.M., Olveira, J.G., Barja, J.L. and Dopazo, C.P. (2000) Diversity of infectious pancreatic necrosis virus strains isolated from fish, shellfish, and other reservoirs in Northwestern Spain. Appl. Environ. Microbiol. 66: 839–643.

    Article  PubMed  CAS  Google Scholar 

  • Dea, S. and Elazhary, M.A.S. (1983) Counter-immunoelectrophoresis for identifi-cation of infectious pancreatic necrosis virus after isolation in cell culture. Can. J. Fish. Aquat. Sci. 40, 2200–2204

    Article  Google Scholar 

  • Desautels, D. and MacKelvie, R.M. (1975) Practical aspects of survival and destruction of infectious pancreatic necrosis virus. J. Fish. Res. Board Can. 32, 523–531.

    Article  Google Scholar 

  • Desselberger, U. (1995) Medical Virology: A practical approach. IRL Press, Oxford. 214 pp.

    Google Scholar 

  • Dobos, P. (1976) Size and structure of the genome of infectious pancreatic necrosis virus. Nuc!. Acids. Res. 3, 1903–1924.

    Article  CAS  Google Scholar 

  • Dobos, P. (1995) The molecular biology of infectious pancreatic necrosis virus (IPNV). In Faisal, M. and Hetrick, F.M.(eds.) Ann. Rev. Fish Dis. Vol. 5 Elsevier Sci. p. 25–54

    Google Scholar 

  • Dobos, P., Hallett, R., Kells, D.T., Sorensen, O. and Rowe, D. (1977) Biophysical studies of infectious pancreatic necrosis virus. J. Virol. 22, 150–159.

    PubMed  CAS  Google Scholar 

  • Dominguez, J., Babin, M., Sanchez, C. and Hedrick, R.P. (1991) Rapid serotyping of infectious pancreatic necrosis virus by one-step enzyme-linked immunosorbent assay using monoclonal antibodies. J. Virol. Methods. 31, 93–103.

    Article  PubMed  CAS  Google Scholar 

  • Dominguez, J., Hedrick, R.P., and Sanchez-Vizcaino, J.M. (1990) Use of monoclonal antibodies for detection of infectious pancreatic necrosis virus by the enzyme-linked immunosorbent assay (ELISA). Dis. Aquas. Org. 8, 157–163.

    Article  CAS  Google Scholar 

  • Dopazo, C.P. (1991) Characterization of fish bimavirus and aquareovirus: Biological, serological and molecular properties. Doctoral dissertation. University of Santiago de Compostela, Spain. (in Spanish)

    Google Scholar 

  • Dopazo, C.P., Hetrick, F.M. and Samal, S.K. (1994) Use of cloned cDNA probes for diagnosis of infectious pancreatic necrosis virus infections. J. Fish Dis. 17, 1–16

    Article  CAS  Google Scholar 

  • Dorson, M. and Torchy, C. (1981) The influence of fish age and water temperature on mortalities of rainbow trout Salmo gairdneri caused by a European strain of infectious pancreatic necrosis virus, J. Fish Dis. 4, 213–221.

    Article  Google Scholar 

  • Dorson, M., Torchy, C. and Michel, C. (1979) Rainbow trout complement fixation used for titration of antibodies against several pathogens. Ann. Rech. Vet. 10, 529–34.

    PubMed  CAS  Google Scholar 

  • Duncan, R. and Dobos, P. (1986) The nucleotide sequence of infectious pancreatic necrosis virus (IPNV) dsRNA segment A reveals one large ORF encoding a precursor polyprotein. Nucl. Acids. Res. 14, 5934–5935.

    Article  PubMed  CAS  Google Scholar 

  • Duncan, R., Mason, C.L., Nagy, E., Leong, J.A. and Dobos, P. (1991) Sequence analysis of infectious pancreatic necrosis virus genome segment B and its encoded VPl protein: A putative RNA-dependent RNA-poiymerase lacking the GLY-ASP-ASP motif. Virology. 181, 541–552.

    Article  PubMed  CAS  Google Scholar 

  • Espinoza, E., Fanas, G., Soler, M. and Kuznar, J. (1985) Identity between infectious pancreatic necrosis virus VR-299 and a Chilean isolate. lntervirology. 24, 58–60.

    Article  CAS  Google Scholar 

  • Fernandez, R.D., Yoshimizu, M., Kimura, T., Ezura, Y., Inouye, K. and Takami, I. (1993) Characterization >of three continuous cell lines from marine fish. J. Aquat. Anim. Health. 5, 127–136.

    Article  Google Scholar 

  • Finlay, J. and Hill, B.J. (1975) The use of the complement fixation test for the rapid typing of infectious pancreatic necrosis virus. Aquaculture 5, 305–310.

    Article  Google Scholar 

  • Follet, J.E. and Schmitt, M.K. (1990) Characterization of a cell line derived from inconnu. J. Aquas. Anim. Health 2, 61–67.

    Article  Google Scholar 

  • Frost, P., Havarstein, L.S., Lygren, B., Stahl, S., Endresen, C. and Christie, K.E. (1995) Mapping of neutralization epitopes on infectious pancreatic necrosis virus. J. Gen. Virol. 76, 1165–1172.

    Article  PubMed  CAS  Google Scholar 

  • Fryer, J.L., McCain, B.B. and Leong, J.C. (1981) A Cell Line Derived From Rainbow Trout (Salmo gairdneri) Hepatoma. Fish Pathol. 15, 193–200.

    Article  Google Scholar 

  • Ganga, M.A., Gonzalez, M.P., Lopez-Lastra, M. and Sandino, A.M. (1994) Polyacrylamide gel electrophoresis of viral genomic RNA as a diagnostic method for infectious pancreatic necrosis virus detection. J. Virol. Methods. 50, 227–236.

    Article  PubMed  CAS  Google Scholar 

  • Gudding, R., Lillehaug, A. and Evensen, 0. (1999) Recent developments in fish vaccinology. Vet. Immunol. Immunopathol. 72, 203–212.

    Article  PubMed  CAS  Google Scholar 

  • Hastein, T. (1996) Surveillance and control of marine fish diseases. In `Comprehensive reports on technical items presented to the Int. Committee or to Regional Commisions’. Office Int. Epizoot. (O1E). p 267–281.

    Google Scholar 

  • Hattori, M., Kodama, H., Ishiguro, S., Honda, A., Mikami, T. and Izawa, H. (1984) In vitro and in vivo detection of infectious pancreatic necrosis virus in fish by enzyme-linked immunosorbent assay. Am. J. Vet. Res. 45, 1876–1879.

    CAS  Google Scholar 

  • Havarstein, L.S., Kailand, K.H., Christie, K.E. and Endresen, C. (1990) Sequence of the large double-stranded RNA segment of the N1 strain of infectious pancreatic necrosis virus: a comparison with other Birnaviridae. J. Gen. Virol. 71, 299–308

    Article  Google Scholar 

  • Hedrick, R.P., Eaton, W., Fryer, J.L., Hah, Y.C., Park, J.W. and Hong, S.W. (1985) Biochemical and serological properties of bimaviruses isolated from fish in Korea. Fish. Pathol. 20, 463–468.

    Article  Google Scholar 

  • Hedrick, R.P., Fryer, J.L., Chen, S.N. and Kou, G.H. (1983a) Characteristics of four bimaviruses isolated from fish in Taiwan. Fish Pathol. 18, 91–97.

    Article  Google Scholar 

  • Hedrick, R.P., McDowell, T.S., Rosemark, R., Aonstein, D. and Lannan, C.N. (1991) Two cell lines from white sturgeon. Trans. Am. Fish. Soc. 120, 528–534.

    Article  Google Scholar 

  • Hedrick, R.P., Okamoto, N., Sano, T. and Fryer, J.L. (1983b) Biochemical characterization of eel virus european. J. Gen. Virol. 64, 1421–1426.

    Article  PubMed  CAS  Google Scholar 

  • Heppell, J., Berthiaume. L., Corbin, T., Tarrab, E., Lecomte, J. and Arella. M. (1993) Comparison of amino acid sequences deduced from a cDNA fragment obtained from infectious pancreatic necrosis virus (IPNV) strains of different serotypes. Virology. 195, 840–844.

    CAS  Google Scholar 

  • Heppell, J., Berthiaume, L., Tarrab, E., Lecomte, J. and Arella. M. (1992) Evidence of genomic variations between infectious pancreatic necrosis virus strains determined by restriction fragment profiles. J. Gen. Virol. 73, 2863–2870.

    Article  PubMed  CAS  Google Scholar 

  • Heppell, J., Tarrab, E., Lecomte, J. Berthiaume. L., and Arella. M. (1995) Strain variability and localization of important epitopes on the major structural protein (VP2) of infectious pancreatic necrosis virus. Virology. 214, 40–49.

    Article  PubMed  CAS  Google Scholar 

  • Hill, B.J. (1982) Infectious pancreatic necrosis virus and its virulence. In: Roberts, R.J. (ed.) Microbial diseases offish. Academic Press p. 91–114.

    Google Scholar 

  • Hill, B.J. and Way, K. (1995) Serological classification of infectious pancreatic necrosis (IPN) virus and other aquatic birnaviruses. Ann. Rev. Fish Dis. 5 Elsevier Sci. 55–77.

    Google Scholar 

  • Hiney, M. (2000) Validation of non-cultured based pathogen detection problems and practical experience. Int. Conf. Risk Analysis in Aquat. Anim. Health (OIE). Paris, Feb 8–10, p. 69.

    Google Scholar 

  • Hosono, N., Suzuki, S. and Kusuda, R. (1996) Genogrouping of bimaviruses isolated from marine fish: A comparison of VP2/NS junction regions on genome segment A. J. Fish-Dis. 19, 295–302.

    Article  CAS  Google Scholar 

  • Hsu, Y.L., Chen, B.S. and Wu, J.L. (1989) Comparison of RNAs and polypeptides of infectious pancreatic necrosis virus isolates from eel and rainbow trout. J. Gen. Virol. 70, 2233–2239.

    Article  PubMed  CAS  Google Scholar 

  • Hsu, Y.L., Chen, B.S. and Wu, J.L. (1993) Demonstration of infectious pancreatic necrosis virus strain VR- 299 in Japanese eel, Anguilla japonica Temminck and Schlegel. J. Fish Dis. 16, 123–129.

    Article  Google Scholar 

  • Hsu, Y.L., Chen, B.S. and Wu, J.L. (1995) Molecular relationships in infectious pancreatic necrosis virus. Virus. Res. 37, 239–2352.

    Article  PubMed  CAS  Google Scholar 

  • Inouye, S. and Hondo, R. (1993) Type differentiation of herpes simplex virus by stringent hybridization of polymerise chain reaction products. Arch. Virol. 129, 311–316.

    Article  PubMed  CAS  Google Scholar 

  • Jarp, J., Gjevre, A.G., Olsen, A.B., and Bruhein, T. (1994) Risk factors for furunculosis, infectious pancreatic necrosis and mortality in post-smolt of Atlantic salmon, Salmo salar L. J. Fish Diseases 18, 67–78.

    Article  Google Scholar 

  • Jiang, Y. and Li, Z. (1987) Isolation of IPN virus from imported rainbow trout (Salmo gairdneri) in the P.R. China. J. Appl. Ichihyol. 3, 191–192.

    Article  Google Scholar 

  • Jung, S.J., Kitamura, S., Kawai, K. and Suzuki, S. (1999) Isolation of different types of bimavirus from ayu Plecoglossus altivelis and amago salmon Oncorhynchus rhodurus cultured in the same geographic area. Dis. Aquat. Org. 38, 87–91.

    CAS  Google Scholar 

  • Kelly, R.K., Miller, H.R., Nielson, O. and Clayton, J.W. (1980) Fish cell culture: characteristics of a continuous fibroblastic cell line from walleye (Stizostedion vitreum vitreum). Can. J. Fish. Aquat. Sci. 37, 1070–1075.

    Article  Google Scholar 

  • Kelly, R.K. and Nielsen, O. (1993) Comparative serology of three recent Canadian isolates of aquatic bimavirus. Fish Pathol. 28, 161–164.

    Article  Google Scholar 

  • Kelly, R.K., Souter, B.W. and Miller, H.R. (1978) Fish cell lines: comparisons of CHSE-214, FHM, and RTG-2 in assaying IHN and IPN viruses. J. Fish. Res. Board Can. 35, 1009–1011.

    Article  Google Scholar 

  • Kimura, T., Yoshimizu, M. and Yasuda, H. (1984). Simple serological diagnosis of infectious pancreatic

    Google Scholar 

  • necrosis by coagglutination test using antibody-sensitized staphylococci. Fish Pathol. 19 25–33. Kwok, S. and Higuchi, R. (1989) Avoiding false positives with PCR. Nature 339 237–238.

    Google Scholar 

  • Lecomte, J., Arella, M. and Berthiaume, L. (1992) Comparison of polyclonal and monoclonal antibodies

    Google Scholar 

  • for serotyping infectious pancreatic necrosis virus (IPNV) strains isolated in eastern Canada. J. Fish Dis. 15 431–436.

    Google Scholar 

  • Ledo, A., Lupiani, B., Dopazo, C.P., Toranzo, A.E. and Barja, J.L. (1990) Fish viral infections in northwest of Spain. Microbiologia. 6, 21–29.

    PubMed  CAS  Google Scholar 

  • Lee, M.K., Blake, S.L., Singer, J.T. and Nicholson, B.L. (1996) Genomic variation of aquatic birnaviruses analyzed with restriction fragment length polymorphisms. Appl. Environ. Microbiol 62, 25132520.

    Google Scholar 

  • Lee, L.H., Ting, L.J., Shien, J.H. and Shien, H.K. (1994) Single-tube, non-interrupted reverse transcription-

    Google Scholar 

  • PCR for detection of infectious bursal disease virus. J. Clin. Microbiol. 32, 1268–1272. Leong, J.C. and Fryer, J.L. (1993) Viral vaccines for aquaculture. Ann. Rev. Fish Dis. 3, 225–240. Lientz, J.C. and Springer, J.E. (1973) Neutralization tests of infectious pancreatic necrosis virus with

    Google Scholar 

  • polyvalent antiserum. J. Wild!. Dis. 9 120–124.

    Google Scholar 

  • Lopez-Lastra, M., Gonzalez, M., Jashes, M. and Sandino, A.M. (1994) A detection method for infectious pancreatic necrosis virus (IPNV) based on reverse transcription (RT)-polymerase chain reaction (PCR). J. Fish Dis. 17, 269–282.

    Article  CAS  Google Scholar 

  • Lorenzen, E., Carstensen, B. and Olesen, N.J. (1999) Inter-laboratory comparison of cell lines for susceptibility to three viruses: VHSV, IHNV and IPNV. Dis. Aquas. Org. 37, 81–88.

    Article  CAS  Google Scholar 

  • Lu, Y.A., Lannan, C.N., Rohovec, J.S. and Fryer, J.L. (1990) Fish cell lines: establishment and characterization of three new cell lines from grass carp (Ctenopharyngodon idella). In Vitro Cell. Dev. Biol. 26: 275–279.

    Google Scholar 

  • Lu, Y., Nerurkar, V.R., Aguirre, A.A., Work, T.M., Balazs, G.H. and Yanagihara, R. (1999) Establishment and characterization of 13 cell lines from a green turtle (Chelonia mydas) with fibropapillomas. In Vitro Cell. Dev. Biol. Anim. 35, 389–393.

    Article  PubMed  CAS  Google Scholar 

  • Macdonald, R.D. (1980) Immunofluorescent detection of double-stranded RNA in cells infected with reovirus, infectious pancreatic necrosis virus, and infectious bursal disease virus. Can. J. Microbiol. 26, 256–61

    Article  PubMed  CAS  Google Scholar 

  • Macdonald, R.D. and Gower, D.A. (1981) Genomic and phenotypic divergence among three serotypes of aquatic bimaviruses (infectious pancreatic necrosis virus). Virology. 114, 187–195.

    Article  PubMed  CAS  Google Scholar 

  • Macdonald, R.D., Moore, A.R. and Souter, B.W. (1983) Three new strains of infectious pancreatic necrosis virus isolated in Canada. Can. J. Microbiol. 29, 137–141.

    Article  PubMed  CAS  Google Scholar 

  • Mason, C.L. (1992) Molecular characterization of the proteins and RNA dependent RNA polymerase of infectious pancreatic necrosis virus, a fish bimavirus. Ph.D. Thesis, Oregon State University. USA.

    Google Scholar 

  • McAllister, P.E. (1997) Susceptibility of 12 lineages of chinook salmon embryo cells (CHSE-214) to four viruses from salmonid fish: Implications for clinical assay sensitivity. J. Aquat. Anim.. Health 9, 291–294.

    Article  Google Scholar 

  • McAllister, P.E. (1988) Viral infections of cultured fish. (Spanish). In Espinosa de los Monteros, J. and Labarta, U. (eds). Patologia en Acuicultura. CAICYT, Madrid. p 37–214.

    Google Scholar 

  • McAllister, P., Newman, M., Sanber, T. and Owens, W. (1984). Isolation of infectious pancreatic necrosis virus (serotype Ab) from diverse species of estuarine fish. Helgolander Meeresunters. 37,.317328.

    Google Scholar 

  • McAllister, P. and Owens, W. (1986) Infectious pancreatic necrosis virus: Protocol for standard challenge to brook trout. Trans. Am. Fish. Soc. 115, 466–470.

    Article  Google Scholar 

  • McAllister, P. and Owens, W. (1993) Recovery of infectious pancreatic necrosis virus from the faeces of wild piscivorous birds. Aquaculture 106, 227–232.

    Article  Google Scholar 

  • McAllister, P.E. and Reyes, X. (1984) Infectious pancreatic necrosis virus: Isolation from rainbow trout, Salmo gairdneri Richardson, imported into Chile. J. Fish Dis. 7, 319–322.

    Article  Google Scholar 

  • McAllister, P.E. and Schill, W.B. (1986) Immunoblot assay: a rapid and sensitive method for identification of salmonid fish viruses. J. Wild!. Dis. 22, 468–474.

    CAS  Google Scholar 

  • McAllister, P.E., Schill, W.B., Owens, W.J. and Hodge, D.L. (1991) Infectious pancreatic necrosis virus: a comparison of methods to detect and identify virus in fluids and in tissues of fish. Proc. 2“ Int Symp. Viruses of Lower Vertebrates. p. 191–201.

    Google Scholar 

  • McMichael, J., Fryer, J.L. and Pilcher, K.S. (1978) An antigenic comparison of three strains of infectious pancreatic necrosis virus of salmonid fish. Aquaculture. 6, 203–210.

    Article  Google Scholar 

  • Melby, H.P. and Christie, K.E. (1994) Antigenic analysis of reference strains and Norwegian field strains of aquatic birnaviruses by the use of six monoclonal antibodies produced against the infectious pancreatic necrosis virus Ni strain. J. Fish Dis. 17, 409–415.

    Article  Google Scholar 

  • M’Gonigle, R.H. (1941) Acute catarrhal enteritis of salmonid fingerlings. Trans. Am. Fish. Soc. 70, 297–303.

    Article  Google Scholar 

  • Moewus-Kobb, L. (1965). Studies with IPN virus in marine hosts. Ann. N.Y. Acad Sci. 126, 328–342. Mullis, K.B. and Faloona, F.A. (1987) Specific synthesis of DNA in vitro via chain reaction. Meth. Enzymol. 155, 335–350.

    Google Scholar 

  • Nakajima, K. and Sorimachi, M. (1994) Serological and biochemical characterization of two birnaviruses: VDV and YAV isolated from cultured yellowtail. Fish Pathol. 29, 183–186

    Article  Google Scholar 

  • Nicholson, B.L. and Caswell, P. (1982) Enzyme-linked immunosorbent assay for identification of infectious pancreatic necrosis virus. J. Clin. Microbiol. 16, 469–472.

    PubMed  CAS  Google Scholar 

  • Nicholson, B.L. and Henchal, E.A. (1978) Rapid identification of infectious pancreatic necrosis virus in infected cell cultures by immunoperoxidase techniques. J. Wildl. Dis. 14, 465–469.

    PubMed  CAS  Google Scholar 

  • Nicholson, B.L. and Pochebit, S. (1981) Antigenic analysis of infectious pancreatic necrosis virus (IPNV) by neutralization kinetics. Develop. BioL Standar. 49, 35–41.

    Google Scholar 

  • Novoa, B., Blake, S., Nicholson, B.L. and Figueras, A. (1995) Comparison of different procedures for serotyping aquatic bimavirus. AppL Environ. Microbiol. 61: 2925–2929.

    PubMed  CAS  Google Scholar 

  • Novoa, B., Figueras, A., Puentes, C.F., Ledo, A. and Toranzo, A.E. (1993a) Characterization of a birnavirus isolated from diseased turbot cultured in Spain. Dis. Aqua:. Org. 15, 163–169.

    Article  Google Scholar 

  • Novoa, B., Toranzo, A.E., Dopazo, C.P., Barja, J.L. and Figueras, A. (19936) Isolation of IPN virus serotype VR-299 from turbot in Europe. Dis. Aquas. Org . 17, 61–65.

    Google Scholar 

  • Olveira, J.G., Cutrin, J.M., Bandin, I., Barja, J.L. and Dopazo, C.P. (1997) Routine diagnosis of IPN virus using RT-PCR and nucleic acid hybridization. EAFP VIIIth Int. Conf. “Diseases of Fish and Shellfish”. Edinburgh. p. 085.

    Google Scholar 

  • Olveira, J.G., Cutrin, J.M., Bandin, I., Barja, J.L. and Dopazo, C.P. (1998) Optimization of a diagnosis method for aquatic bimaviruses by RT-PCR. 4`b Int. Symp. Viruses of Lower Vertebrates, Weymouth, UK. p. 04.

    Google Scholar 

  • Pérez-Prieto, S.I., Rodriguez, S., Garcia-Rosado, E., Castro, D., Alvarez, M.C. and Borrego, J.J. (1999) Virus susceptibility of the fish cell line SM-1 derived from gilt-head seabream. Dis. Aquas. Org. 35, 149–153.

    Google Scholar 

  • Peters, F. and Neukirch, M. (1986) Transmission of some fish pathogenic viruses by the heron, Ardea cinerea. J. Fish Dis. 9, 539–544.

    Article  Google Scholar 

  • Pryde, A., Melvin, W.T. and Munro, A.L. (1993) Nucleotide sequence analysis of the serotype-specific epitope of infectious pancreatic necrosis virus. Arch. Virol. 129, 287–293.

    Article  PubMed  CAS  Google Scholar 

  • Regenmortel, M.H.van., Fauquet, C.M., Bishop, D.H.L., Carstens, E.B., Estes. M.K., Lemon, S.M., Maniloff, J., Mayo, M.A., McGeoch, D.J., Pringle, C.R. and Wickner, R.B. (Eds) (2000) Virus taxonomy. Classification and nomenclature of viruses. Acad. Press. 1162 pp.

    Google Scholar 

  • Reno, P.W. (1999) Infectious pancreatic necrosis and associated aquatic bimaviruses. In Woo, P.T. & Bruno, D.W. (eds.). Fish Diseases and Disorders, V. 3: Viral, Bacterial and Fungal Infections. CABI Publ. UK. p. 1–55.

    Google Scholar 

  • Rimstad, E., Homes, E., Olsvik, O. and Hyllseth, B. (1990a) Identification of a double-stranded RNA virus by using polymerase chain reaction and magnetic separation of the synthesized DNA segments. J. Clin. Microbiol. 28, 2275–2278.

    PubMed  CAS  Google Scholar 

  • Rimstad, E., Krona, R., Homes, E., Olsvik, O. and Hyllseth, B. (1990b) Detection of infectious pancreatic necrosis virus (IPNV) RNA by hybridization with an oligonucleotide DNA probe. Vet. Microbiol. 23, 211–219.

    Article  PubMed  CAS  Google Scholar 

  • Rodak, L., Pospisil, Z., Tomanek, J., Vesely, T., Obr, T. and Valicek, L. (1988) Enzyme-linked immunosorbent assay (ELISA) detection of infectious pancreatic necrosis virus (1PNV) in culture fluids and tissue homogenates of the rainbow trout, Salmo gairdneri Richardson. J. Fish Dis. 11, 225–235.

    Article  Google Scholar 

  • Rodriguez, S., Vilas, M.P., Alonso, M. and Perez, S.I. (1995) Study of a viral-dual infection in rainbow trout (Oncorhynchus mykiss) by seroneutralization, western blot and polymerase chain reaction assays. Microbiologia. 11, 461–470.

    PubMed  CAS  Google Scholar 

  • Rodriguez, S., Vilas, M.P., Gutiérrez, M.C., Pérez-Prieto, S.I., Sarasquete, M.C. and Rodriguez, R.B. (1997) Isolation and preliminary characterization of a bimavirus from the sole Solea senegalensis in southwest Spain. J. Aquas. Anim. Health 9, 295–300.

    Article  Google Scholar 

  • Saiki, R.K., Scharf, S., Fallona, F.A., Mullis, K.B., Horn, G.T., Erlich, H.A. and Amheim, N. (1985) Enzymatic amplification of ß-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230, 1350–1354.

    CAS  Google Scholar 

  • Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular cloning. A laboratory manual (2`d ed.). Cold Spring Harbor Lab. Press. New York.

    Google Scholar 

  • Sano, T. (1971) Studies on viral diseases of Japanese fishes-I. Infectiuos pancreatic necrosis of rainbow trout: first isolation from epizootics in Japan. Bull. Jpn. Soc. Fish. 37, 495–498.

    Article  Google Scholar 

  • Sano, T., Okamoto, N. and Nishimura, T. (1981) A new viral epizootic of Anguilla japonica Temminek and Schlegel. J. Fish Dis. 4, 127–139.

    Article  Google Scholar 

  • Sanz, F. and Coll, J. (1992) Techniques for diagnosing viral diseases of salmonids. Dis. Aquas. Org. 13: 211–232.

    Google Scholar 

  • Seo, J.J., Heo, G.J. and Lee, C.H. (1998) Characterization of aquatic bimavirus isolated from rockfish (Sebastes schlegeli) cultured in Korea Bull Eur. Assoc. Fish Pathol. 18, 87–92.

    Google Scholar 

  • Shankar, K.M. and Yamamoto, T. (1994) Epitope analysis of infectious pancreatic necrosis virus (1PNV) isolated from lake trout, Salvelinus namaycush (Walbaum), by monoclonal antibodies. J Fish Dis. 17: 471–482.

    Article  CAS  Google Scholar 

  • Small, D.A., Bruno, D.W., Dear, G., McFarlane, L.A. and Ross, K. (1992) Infectious Pancreatic Necrosis (IPN) virus Sp serotype in farmed Atlantic salmon, Salmo salar L., post-smolts associated with mortality and clinical disease. J. Fish Dis. 15, 77–83

    Article  Google Scholar 

  • Sohn, S.G., Park, M.A., Do, J.W., Choi, J.Y. and Park, J.W. (1995) Bimavirus isolated from cultured flounder in Korea. Fish. Pathol. 30, 279–280.

    Article  Google Scholar 

  • Sonstergard, R.A. and McDermott, L.A. (1972) Epidemiological model for passive transfery of IPNV by homeotherms. Nature, 237, 104–105.

    Article  Google Scholar 

  • Sonstergard, R.A., McDermott, L.A. and Sonstergard, K.S. (1972) Isolation of infectious pancreatic necrosis virus from white suckers (Catostomus commersoni). Nature 236, 174–175.

    Article  Google Scholar 

  • Swanson, R.N. and Gillespie, J.H. (1981) An indirect fluorescent antibody test for the rapid detection of infectious pancreatic necrosis virus in tissues. J. Fish. Dis. 4, 309–315.

    Article  Google Scholar 

  • Takeda, N., Sakae, K., Agboatwalla, M., Isomura, S., Hondo, R. and Inouye, S. (1994) Differentiation between wild and vaccine-derived strains of poliovirus by stringent microplate hybridization of PCR products. J Clin. Microbiol. 32, 202–204.

    PubMed  CAS  Google Scholar 

  • Taksdal, T. and Thorud, K. (1999) Evaluation of a rapid coagglutination (COA) test for the detection of infectious pancreatic necrosis virus (IPNV) in tissue samples of Atlantic salmon, Salmo salar L. J. Fish Dis. 22, 117–124.

    Article  Google Scholar 

  • Tarrab, E., Berthiaume, L., Grothe, S., O’Connor-McCourt, M., Heppell, J. and Lecomte, J. (1995) Evidence of a major neutralizable conformational epitope region on VP2 of infectious pancreatic necrosis virus. J. Gen. Virol. 76, 551–558.

    Article  PubMed  CAS  Google Scholar 

  • Tarrab, E., Berthiaume, L., Heppell, J., Arella, M. and Lecomte, J. (1993) Antigenic characterization of serogroup `A’ of infectious pancreatic necrosis virus with three panels of monoclonal antibodies. J. Gen. Virol. 74: 2025–2030.

    Article  PubMed  CAS  Google Scholar 

  • Tisdall, D.J. and Phipps, J.C. (1987) Isolation and characterization of a marine bimavirus from returning quinnat salmon (O. tshawytscha) inthe south island of New Zealand. NZ Vet. J. 35, 217–218.

    Article  CAS  Google Scholar 

  • Underwood, B.O., Smale, C.J., Brown, F. and Hill, B.J. (1977) Relationship of a virus from Tellina tenuis to infectious pancreatic necrosis virus. J Gen. Virol. 36, 93–109.

    Article  PubMed  CAS  Google Scholar 

  • Vaughan, L.M., Liljestroen, P., Todd, D., Lillehaug, A. and Ramstad, A. (1998) Recombinant vaccines against infectious pancreatic necrosis (IPN) in salmonid fish. In Barthel, K.G., Barth, H., BohleCarbonel, M., Fragakis, C., Lipiatou, E., Martin, P., Oilier, G. and Weydert, M., (eds). Procs. 3rd Eur. Mar. Sci. Technol. Conf., MAST Conf Luxembourg Eur. Commision. DG-12 Science, Res. Develop. 6, 41–44.

    Google Scholar 

  • Vâzquez-Bra0as, M., Morales, J.C. and Estepa, A. (1994) A sandwich ELISA to detect VHSV and IPNV in turbot. Aquaculture. Int. 2, 206–217.

    Google Scholar 

  • Wang, W.S., Lee, J.S., Blake, S.L. and Nicholson, B.L. (1995) Developing the polymerase chain reaction technique to detect aquatic bimaviruses. Taiwan J. Vet. Med. Anim. Husb. 65, 167–180.

    Google Scholar 

  • Wang, W.S., Wi, Y.L. and Lee, J.S. (1997) Single-tube, non-interrupted reverse transcription PCR for detection of infectious pancreatic necrosis virus. Dis. Aquat. Org. 28, 229–233.

    Article  Google Scholar 

  • Westermeier, R. (1997) (rd ed) Electrophoresis in practice. VCH, Wiley Co. FRG. 331 pp. Wiedbrauk, D.L. and Farkas, D.H. (1995) Molecular methods for virus detection. Acad. Press. 386 pp.

    Google Scholar 

  • Williams, K., Blake, S., Sweeney, A., Singer, J.T. and Nicholson, B.L. (1999) Multiplex reverse transcriptase PCR assay for simultaneous detection of three fish viruses. J. Clin. Microbiol. 37, 4139–4141.

    PubMed  CAS  Google Scholar 

  • Wolf, K. (1988) Fish viruses and fish viral diseases. Cornell Univ. Press, New York. 476 pp.

    Google Scholar 

  • Wolf, K. and Quimby, M.C. (1971) Salmonid viruses: infectious pancreatic necrosis virus. Morphology, pathology and serology of first European isolations. Arch. Gesamte. Virusforsch. 34, 144–156.

    Article  PubMed  CAS  Google Scholar 

  • Wolf, K., Quimby, M.C. and Bradford, (1963) Egg-associated transmission of IPN virus of trout. Virology. 21, 317–321.

    Article  Google Scholar 

  • Wood, E.M., Snieszko, S.F. and Yasutake, W.T. (1955) Infectious pancreatic necrosis in brook trout. Arch. Pathol. 60: 26–28.

    Google Scholar 

  • World Health Organization (1991) Manual for the virological investigation of poliovirus. WHO, Geneva.

    Google Scholar 

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  1. Departamento de Microbioloxía e Parasitoloxía, Instituto de Acuicultura, Universidade de Santiago de Compostela, Spain

    Carlos P. Dopazo

  2. Facultade de Bioloxía, Universidade de Santiago de Compostela, Spain

    Juan L. Barja

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  1. FRS Marine Laboratory, Victoria Road, PO Box 101, AB11 9DB, Aberdeen, Scotland

    Carey O. Cunningham

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Dopazo, C.P., Barja, J.L. (2002). Diagnosis and Identification of IPNV in Salmonids by Molecular Methods. In: Cunningham, C.O. (eds) Molecular Diagnosis of Salmonid Diseases. Reviews: Methods and Technologies in Fish Biology and Fisheries, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2315-2_2

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