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Metabolic engineering of lactic acid bacteria for the production of nutraceuticals

  • Chapter
Lactic Acid Bacteria: Genetics, Metabolism and Applications

Abstract

Lactic acid bacteria display a relatively simple and well-described metabolism where the sugar source is converted mainly to lactic acid. Here we will shortly describe metabolic engineering strategies on the level of sugar metabolism, that lead to either the efficient re-routing of the lactococcal sugar metabolism to nutritional end-products other than lactic acid such as L-alanine, several low-calorie sugars and oligosaccharides or to enhancement of sugar metabolism for complete removal of (undesirable) sugars from food materials. Moreover, we will review current metabolic engineering approaches that aim at increasing the flux through complex biosynthetic pathways, leading to the production of the B-vitamins folate and riboflavin. An overview of these metabolic engineering activities can be found on the website of the Nutra Cells 5th Framework EU-project (www.nutracells.com). Finally, the impact of the developments in the area of genomics and corresponding high-throughput technologies on nutraceutical production will be discussed.

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Authors and Affiliations

  1. Wageningen Centre for Food Sciences, The Netherlands

    Jeroen Hugenholtz, Wilbert Sybesma, Masja Nierop Groot, Wouter Wisselink, Gerrit Eggink & Michiel Kleerebezem

  2. NIZO Food Research, Ede, The Netherlands

    Jeroen Hugenholtz, Eddy J. Smid, Tanja Jansen & Michiel Kleerebezem

  3. University College Cork, Ireland

    Kay Burgess & Douwe van Sinderen

  4. Universite Catholic de Louvain-la-Neuve, Belgium

    Victor Ladero & Pascal Hols

  5. INRA Jouy-en Josas, France

    Jean-Christophe Piard

  6. ATO-DLO, Wageningen, The Netherlands

    Wouter Wisselink & Gerrit Eggink

  7. CERELA, Tucuman, Argentina

    Graciela Savoy & Fernando Sesma

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  1. Jeroen Hugenholtz
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  2. Wilbert Sybesma
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  7. Jean-Christophe Piard
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  9. Eddy J. Smid
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  13. Pascal Hols
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  14. Michiel Kleerebezem
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Editors and Affiliations

  1. Wageningen Center for Food Sciences, and NIZO food research, the Netherlands

    R. J. Siezen

  2. University of Groningen, the Netherlands

    J. Kok

  3. Wageningen Center for Food Sciences, and Wageningen University, the Netherlands

    T. Abee

  4. TNO Nutrition and Food Research, Zeist, the Netherlands

    G. Schasfsma

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Hugenholtz, J. et al. (2002). Metabolic engineering of lactic acid bacteria for the production of nutraceuticals. In: Siezen, R.J., Kok, J., Abee, T., Schasfsma, G. (eds) Lactic Acid Bacteria: Genetics, Metabolism and Applications. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2029-8_13

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  • DOI: https://doi.org/10.1007/978-94-017-2029-8_13

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