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Immobilization of Cells and Enzymes Using Electrostatic Droplet Generation

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Fundamentals of Cell Immobilisation Biotechnology

Part of the book series: Focus on Biotechnology ((FOBI,volume 8A))

Abstract

Selection of support material and method of immobilization is made by weighing the various characteristics and required features of the cell/enzyme application against the properties and limitations of the combined immobilization and support. A number of practical aspects should be considered before embarking on experimental work to ensure that the final immobilized cell or enzyme preparation is fit for the planned purpose or application to operate at optimum effectiveness. The mechanism of alginate droplet formation as well as experimental parameters for producing small hydro gel beads using an electrostatic droplet generator was investigated. It was found that microbead size was a function of needle diameter, charge arrangement (i.e. electrode geometry and spacing) and strength of the electric field. The process of alginate droplet formation under the influence of electrostatic forces was assessed with an image analysis/video system and revealed distinct stages; after a voltage was applied the liquid meniscus at the needle tip was distorted from a spherical shape into an inverted cone-like shape. Alginate solution flowed into this cone at an increasing rate causing formation of a neck-like filament. When this filament broke away, producing small droplets, the meniscus relaxed back to a spherical shape until flow of the alginate caused the process to start again. Various cells suspensions and enzymes were subjected to a high voltage immobilization process in order to assess the effects of electric fields on animal cell viability and enzyme activity. There was no detectable loss in cell viability or enzyme activity after the voltage was applied.

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Author information

Authors and Affiliations

  1. Department of Chemical Engineering, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000, Belgrade, Yugoslavia

    Branko M. Bugarski & Bojana Obradovic

  2. Department of Food Technology and Biochemistry, Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11081, Belgrade-Zemun, Yugoslavia

    Viktor A. Nedovic

  3. ENITIAA, Rue de la Géraudiere, BP 82 225, Nantes, 44322, France

    Denis Poncelet

Authors
  1. Branko M. Bugarski
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  2. Bojana Obradovic
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  3. Viktor A. Nedovic
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  4. Denis Poncelet
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Editor information

Editors and Affiliations

  1. University of Belgrade, Belgrade, Serbia and Montenegro

    Viktor Nedović

  2. Free University of Brussels, Brussels, Belgium

    Ronnie Willaert

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© 2004 Springer Science+Business Media Dordrecht

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Bugarski, B.M., Obradovic, B., Nedovic, V.A., Poncelet, D. (2004). Immobilization of Cells and Enzymes Using Electrostatic Droplet Generation. In: Nedović, V., Willaert, R. (eds) Fundamentals of Cell Immobilisation Biotechnology. Focus on Biotechnology, vol 8A. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1638-3_15

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  • DOI: https://doi.org/10.1007/978-94-017-1638-3_15

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-6534-6

  • Online ISBN: 978-94-017-1638-3

  • eBook Packages: Springer Book Archive

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