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Nutritional Improvement of Lupin Seed Protein Using Gene Technology

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Applied Genetics of Leguminosae Biotechnology

Part of the book series: Focus on Biotechnology ((FOBI,volume 10B))

Abstract

The nutritive value of lupin, an important grain legume for animal production, has been improved by the transformation of a chimeric gene specifying seed-specific expression of a sulfur-rich sunflower seed albumin (SSA). This gene encodes a protein which contains 16% methionine and 8% cysteine residues and the protein was expressed in transgenic seeds at a level of 5% of total soluble seed protein. The expression of the SSA gene in lupin seeds caused an overall increase in sulfur containing amino acids of 19%, while methionine levels were doubled. The improved nutritive value of these transgenic lupin seeds was demonstrated in feeding trials with rats, chickens, and sheep.

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References

  • Altenbach S B, Kuo C C, Staraci L C, Pearson K W, Wainwright C, Georgescu A and Townsend J (1992) Accumulation of a Brazil nut albumin in seeds of transgenic canola results in enhanced levels of seed protein methionine. Plant Mol. Biol, 18: 235–246.

    Article  PubMed  CAS  Google Scholar 

  • Eggum B O, Tomes G, Beames R M and Datta F U (1993) Protein and energy evaluation with rats of seed from 11 lupin cultivars. Anim. Feed Sci. Technoi, 43: 109–119.

    Article  Google Scholar 

  • Falco S C, Guida T, Locke M, Mauvais J, Sanders C, Ward R T and Webber P (1995) Transgenic canola and soybean seeds with increased lysine. Bio/Technology, 13: 577–582.

    Article  PubMed  CAS  Google Scholar 

  • Gamborg O L, Miller R A and Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell. Res., 50: 151–158.

    Article  PubMed  CAS  Google Scholar 

  • Hamblin J, Barton J, Atkins C, Jones M, Smith P, Wylie S, Schroeder H, Molvig L, Tabe L, Higgins T J V (1998) The development and status of transgenic pulses in Australia. In: 3rd European conference on grain legumes. Opportunities for high quality, healthy and added-value crops to meet European demands, 70–71.

    Google Scholar 

  • McNabb W C, Spencer D S, Higgins T J and Barry T N (1994) In vitro rates of rumen proteolysis of ribulose-1, 5-biphosphate carboxylase (rubisco) from lucerne leaves, and of ovalbumin, vicilin and sunflower seed albumin 8 storage proteins. J. Sci. Food Agrie, 64: 53–61.

    Article  CAS  Google Scholar 

  • Molvig L, Tabe L M, Eggum B O, Moore A E, Craig S, Spencer D and Higgins T J V (1997) Enhanced methionine levels and increased nutritive value of seeds of transgenic lupins (Lupinus angustifolius L.) expressing a sunflower seed albumin gene. Proc. Natl. Acad. Sci. USA, 94: 8393–8398.

    Article  PubMed  CAS  Google Scholar 

  • Molvig L, Tabe L M, Hamblin J, Ravindran V, Bryden W L, Smith D M, Kissil G W M, Lupatsch I, White C L and Higgins T J V (1999) Increased nutritional quality of seeds of transgenic lupins (Lupinus angustifolius L.) expressing a sunflower seed albumin gene. In: Plant tissue culture at the edge of the new millennium, Proceedings of the International Association for Plant Tissue Culture and Biotechnology (Australian Branch), Vlth National Meeting, 109–114.

    Google Scholar 

  • Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15: 473–497.

    Article  CAS  Google Scholar 

  • Pigeare A, Abernethy D, Smith P M, Simpson K, Fletcher N, Lu C Y, Atkins C A and Cornish E (1997) Transformation of a grain legume (Lupinus angustifolius L.) via Agrobacterium tumefaciens-mediatcd gene transfer to shoot apices. Mol. Breed., 3: 341–349.

    Article  Google Scholar 

  • Ravindran V, Bryden W L, Tabe L M, Higgins T J V and Molvig L (1998) Evaluation of high-methionine transgenic lupins in broiler diets. Proc. Aust. Poult. Sci. Symp., 10: 208.

    Google Scholar 

  • Reis P J (1979) Effects of amino acids on the growth and properties of wool. In: Physiological and environmental limitations to wool growth (Eds Black J L and Reis P J), University of New England Publishing Unit, Australia, 223–242.

    Google Scholar 

  • Saalbach I, Pickardt T, Waddell D R, Hillmer S, Scheider O and Muntz K (1995) The sulphur-rich Brazil nut 2S albumin is specifically formed in transgenic seeds of the grain legume Vicia narbonensis. Euphytica, 85: 181–192.

    Article  CAS  Google Scholar 

  • Shetty K, Asano Y and Oosawa K (1992) Stimulation of in vitro shoot organogenesis in Glycine max (Merrill.) by allantoin and amides. Plant Sci., 81: 245–251.

    Article  CAS  Google Scholar 

  • White C L, Tabe L M, Dove H, Hamblin J, Young P, Phillips N, Taylor R, Gulati S, Ashes J and Higgins T J V (2001) Increased efficiency of wool growth and liveweight gain in Merino sheep fed transgenic lupin seed containing sunflower albumin. J. Sci. Food. Agric, 81: 147–154.

    Article  CAS  Google Scholar 

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© 2003 Springer Science+Business Media Dordrecht

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Molvig, L. et al. (2003). Nutritional Improvement of Lupin Seed Protein Using Gene Technology. In: Jaiwal, P.K., Singh, R.P. (eds) Applied Genetics of Leguminosae Biotechnology. Focus on Biotechnology, vol 10B. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0139-6_14

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  • DOI: https://doi.org/10.1007/978-94-017-0139-6_14

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-6369-4

  • Online ISBN: 978-94-017-0139-6

  • eBook Packages: Springer Book Archive

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