Abstract
Classical rabies vaccine strains (genotype 1) protect inefficiently against rabies-related lyssaviruses (other genotypes) and in particular against the Mokola virus (genotype 3). DNA vaccine modern approach was used to produce Mokola valency. Purified Plasmid vector was injected into each anterior tibialis muscle of BALB/c mice. It appeared that the Mokola DNA vaccine has induced a sustained level of VNAb, a T helper cell response and confered protection against Mokola virus challenge. Therefore, it seems that the DNA vaccine approach is a very promising technology, which needs further investigations for a possible application.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bourhy H, Kissi B and Tordo N (1993) Molecular Diversity of the Lyssavirus genus. Virology 194, 70–81.
Tordo N, Bourhy H, Sather S and Ollo R (1993) Structure and Expression in Baculovirus of the Mokola Virus Glycoprotein: An Efficient Recombinant Vaccine. Virology 194, 59–69.
Tang D, De Vit M and Johnston SA (1992) Genetic immunization is a simple method for eliciting an immune response. Nature 356, 152–154.
Yankauckas MA, Morrow JE, Parker SE, Abai A, Rhodes GH, Dwarki VJ and Gromkowski SH (1993) Long-Term Anti-Nucleoprotein Cellular and Humoral Immunity Is Induced by Intramuscular Injection of Plasmid DNA Containing NP gene. DNA and Cell Biology 12, 771–776.
Wang B, Ugen KE, Srikantan V, Agadjanyan MG, Dang K, Refaeli Y, Sato AI, Boyer J, Williams WV and Weiner DB (1993) Gene inoculation generates immune responses against human immunodeficiency virus type 1. Proc. Natl. Acad. Sci. USA 90, 4156–4160.
Xiang ZQ, Spitalink S, Tran M, Wunner WH, Cheng J and Ertl HCJ (1994) Vaccination with a Plasmid Vector Carrying the Rabies Virus Glycoprotein Gene Induces Protective Immunity against Rabies Virus. Virology 199, 132–140.
Smith J, Yager P, Baer G (1996) A rapid fluorescent focus inhibition test (RFFIT) for determining virus-neutralizing antibody. In: Meslin FX, Kaplan M and Koprowski H, Laboratory Techniques in Rabies, pp 181–189, WHO, Geneva, Switzerland.
Perrin P, DeFranco M, Jallet C, Fouque F, Morgeaux S, Tordo N and Colle JH (1996) The antigen-specific cell-mediated immune response in mice is suppressed by infection with pathogenic lyssaviruses. Res. Virol. 147, In press.
Fynan EF, Webster RG, Fuller DH, Haynes JR, Santoro JC and Robinson HL (1993) DNA vaccines: Protective immunizations by parenteral, mucosal, and gene-gun inoculations. Proc. Natl. Acad. Sci. USA 90, 11478–11482.
Ulmer JB, Donnelly JJ, Parker SE, Rhodes GH, Felgner PL, Dwarki VJ, Gromkowski SH, Deck RR, De Witt CM, Friedman A, Hawe LA, leander KR, Martinez D, Perry HC, Shiver JW, Montgomery DL and Liu MA (1993) Heterologous Protection Against Influenza by Injection of DNA Encoding a Viral Protein. Science 259, 1745–1749.
Xiang ZQ, Knowles BB, McCarrick JW and Ertl HCJ (1995) Immune Effector Mechanisms Required for Protection to Rabies Virus. Virology 214, 398–404.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Bahloul, C., Fouque, F., Tordo, N., Jacob, Y., Perrin, P. (1997). Toward a DNA Vaccine Against Lyssa Viruses. In: Carrondo, M.J.T., Griffiths, B., Moreira, J.L.P. (eds) Animal Cell Technology. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5404-8_7
Download citation
DOI: https://doi.org/10.1007/978-94-011-5404-8_7
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6273-2
Online ISBN: 978-94-011-5404-8
eBook Packages: Springer Book Archive