Abstract
Primary hepatocytes of rats and dogs formed spheroids in the pores of Polyurethane foam (PUF) used as a culture substratum. The hepatocytes in monolayer and spheroid cultures of stationary state converted lidocaine to monoethylglycinexylidide (MEGX) which was N-deethylation of lidocaine. These metabolic activities of the hepatocytes/spheroid stationary culture system were 1.5∼2.0-fold higher than those of the hepatocyte monolayer culture for 10 days. Consequently, PUF/spheroid culture maintained high drug metabolizing activity for more than 10 days and seems to be promising as a drug metabolism simulator which will be used for cytotoxicity tests and drug screening.
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© 1997 Springer Science+Business Media Dordrecht
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Funatsu, K., Nakazawa, K., Mizumoto, H., Takenaka, K., Matsushita, T. (1997). Development of Drug Metabolism Simulator Using Three-Dimensional Culture of Hepatocytes of Rats and Dogs. In: Carrondo, M.J.T., Griffiths, B., Moreira, J.L.P. (eds) Animal Cell Technology. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5404-8_14
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DOI: https://doi.org/10.1007/978-94-011-5404-8_14
Publisher Name: Springer, Dordrecht
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