Skip to main content
SpringerLink
Log in

Cell Based Assays for Drug Screening

  • Chapter
Animal Cell Technology

  • 28 Accesses

Abstract

A CRE-alkaline Phosphatase reporter construct has been introduced into a mammalian cell line to investigate signal transduction pathways used by the calcitonin receptor. Calcitonin activated alkaline Phosphatase production in the CRE-reporter cell line.

indicated coupling to the Gαs protein and activation could be inhibited by antagonists. These cells allow functional screening and analysis of possible novel drugs. The genetic manipulation of the cAMP/CRE/CREB pathway for cGMP sensing using the CRE-alkaline Phosphatase cell based assay shall be discussed. We show that by altering the amino acid sequences of the CREB within the substrate recognition region we may be able to alter the substrate specificity of CREB for phosphorylation by PKA and PKG.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 259.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Walters U (1989) Rev. Physiol. Biochem. Physiol. 113, 41–88.

    Article  Google Scholar 

  2. Kennelly P J & Krebs E G (1991) J. Biol. Chem. 266, 15555–15558.

    CAS  PubMed  Google Scholar 

  3. Wigler M, Sweet R, Sim G K etal (1979) Cell 16, 777–785.

    Article  CAS  PubMed  Google Scholar 

  4. Colbran J L, Francis S H, Leach A B, Thomas M K, Jiang H, McAllister L M & Corbin J D (1992) J. Biol. Chem. 267 14 9589–9594.

    CAS  PubMed  Google Scholar 

  5. Gonzalez G A & Montminny M R (1989) Cell 59, 675–680.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological Sciences, University of Paisley, High Street, Paisley, Scotland, PA1 2BE, UK

    Elizabeth Keenan, Helen Kruger, Balwinder Sambi & Dr. John S. McLean

Authors
  1. Elizabeth Keenan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Helen Kruger
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Balwinder Sambi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dr. John S. McLean
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. IBET, Oeiras, Portugal

    Manuel J. T. Carrondo  & José L. P. Moreira  & 

  2. CAMR, Porton Down, Wiltshire, UK

    Bryan Griffiths

Rights and permissions

Reprints and permissions

Copyright information

© 1997 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Keenan, E., Kruger, H., Sambi, B., McLean, J.S. (1997). Cell Based Assays for Drug Screening. In: Carrondo, M.J.T., Griffiths, B., Moreira, J.L.P. (eds) Animal Cell Technology. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5404-8_13

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-5404-8_13

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6273-2

  • Online ISBN: 978-94-011-5404-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation