Abstract
We have investigated the potential use of immortalised rat hepatocyte lines as an alternative to primary rat hepatocytes for drug metabolism and screening. Primary hepatocytes are widely used in the pharmaceutical industry for in vitro studies because established hepatoma cell lines no longer express the relevant drug-metabolising enzyme systems. Immortalised hepatocyte lines have been isolated by transfection of primary cells with SV40 early region DNA. These lines have been investigated for the retention of drug-metabolising enzymes such as glutathione-S-transferases, cytochrome P450s and bilirubin UDPGT. We have shown that immortalised lines retain a drug-metabolising capability but that these enzymes demonstrate the instability of expression with time in culture which is also a feature of primary cells.
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© 1997 Springer Science+Business Media Dordrecht
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MacDonald, C. et al. (1997). Metabolism and Toxicological Studies in Immortalised Rat Hepatocyte Cell Lines. In: Carrondo, M.J.T., Griffiths, B., Moreira, J.L.P. (eds) Animal Cell Technology. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5404-8_12
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DOI: https://doi.org/10.1007/978-94-011-5404-8_12
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6273-2
Online ISBN: 978-94-011-5404-8
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