Abstract
Bovine polyoma virus (BPyV) has been detected with relatively high frequency in clinically normal calves indicating widespread presence of this virus in cattle. Polyoma virus contamination of a wide range of cell cultures has been linked to the supplementation of culture medium with contaminated calf serum. BPyV has been detected in approximately 70% of batches of calf serum tested (Schuurman et al., 1991). This high incidence of contamination raises safety concerns for biopharmaceuticals.
We have established a reliable and sensitive PCR assay which can detect 10 pg BPyV DNA. This was developed using a BPyV infected cell line (a gift from D. Westcott, CVL) as a source of positive control DNA, and our own stock of MDBK cells as a source of negative control DNA. This assay can be used as a routine screen for the detection of BPyV in established cell lines and continuous cell cultures.
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References
Westcott, D. G. F., Ticehurst, J., Chaplin, M., Lukey, J.R & Lucas, M. (1987) The Isolation of a Virus Resembling a Polyomavirus from Normal Calves. Veterinary Microbiology 15 175–180.
Schuunnan, B., van Steenis, B., van Strien, A., van der Noordaa, J. & Sol, C. Frequent Detection of Bovine Polyomavirus in Commercial Batches of Calf Serum by Using the Polymerase Chain Reaction. Journal General Virology (1991) 72 2739–2745 .
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© 1997 Springer Science+Business Media Dordrecht
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Proffitt, J., Jarvis, K., Martin, C. (1997). Bovine Polyoma Virus Detection by Routine PCR Screening. In: Carrondo, M.J.T., Griffiths, B., Moreira, J.L.P. (eds) Animal Cell Technology. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5404-8_10
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DOI: https://doi.org/10.1007/978-94-011-5404-8_10
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