Abstract
A micromachined continuous flow reactor has been successfully used to perform the polymerase chain reaction (PCR) at ultra high speed. The device is analogous to an electronic amplifier, and relies on the movement of sample through thermostated temperature zones on a glass microchip. Input and output of material (DNA) is continuous, and amplification independent of input concentration. A 20-cycle PCR amplification of a 176-base pair fragment from the Neisseria Gonorrhoeae DNA gyrase gene (gyrA) was performed at various flow rates, resulting in a total reaction time of between 90 seconds and 18.7 minutes.
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References
Mullis, K., et al. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. in Cold Spring Harbor Symposia on Quantitative Biology. 1986.
Wittwer, C.T. and Garling, D.J., Rapid cycle DNA amplification: time and temperature optimization. BioTechniques, 1991. 10: p. 76–83.
Cheng, J., Shoffner, M.A., Mitchelson, K.R., Kricka, L.J., and Wilding, P., Analysis of ligase chain reaction products amplified in a silicon glass chip using capillary electrophoresis. Journal Of Chromatography A., 1996. 732(1): p. 151–158.
Burns, M.A., et al., Microfabricated structures for integrated DNA analysis. Proceedings Of The National Academy Of Sciences Of The United States Of America, 1996. 93(11): p. 5556–5561.
Cheng, J., Shoffner, M.A., Hvichia, G.E., Kricka, L.J., and Wilding, P., Chip PCR. II. Investigation of Different PCR amplification systems in microfabricated silicon-glass chips. Nucleic Acids Research, 1996. 24(2): p. 380–385.
Northrup, M.A., Beeman, B., Hadley, D., Landre, P., and Lehew, S. Integrated miniature DNA-based analytical instrumantation. in μTAS’ 96. 1996. Basel: AMI.
Woudenberg, T.M., Winn-Deen, E.S., and Albin, M. High-density PCR and beyond, in μTAS’ 96. 1996. Basel: AMI.
Woolley, A.T., et al., Functional integration of PCR amplification and capillary electrophoresis in a microfabricated DNA analysis device. Analytical Chemistry, 1996. 68: p. 4081–4086.
Kopp, M.U., deMello, A.J., and Manz, A., Chemical Amplification: Continuous Flow PCR on a Chip. Science,. 280: p. 1046–1048.
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© 1998 Springer Science+Business Media Dordrecht
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Kopp, M.U., Luechinger, M.B., Manz, A. (1998). Continuous Flow PCR on A Chip. In: Harrison, D.J., van den Berg, A. (eds) Micro Total Analysis Systems ’98. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5286-0_2
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DOI: https://doi.org/10.1007/978-94-011-5286-0_2
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