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Histone-DNA Contacts in Structure/Function Relationships of Nucleosomes as Revealed by Crosslinking

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Structural Biology and Functional Genomics

Part of the book series: NATO Science Series ((ASHT,volume 71))

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Abstract

We describe studies of histone-DNA contacts in the nucleosome using the method of covalent zero length protein-DNA crosslinking. These studies show that in intact nuclei isolated from different sources the linear sequential arrangement of histone-DNA contacts in the nucleosomal core is essentially the same. However, the relative strength of certain contacts varies and correlates with the level of chromatin activity and condensation. These altered contacts are located in the sharply bent regions of the nucleosomal DNA and are supposed to be sensetive to the structural changes which may occur during nucleosome functions. Studies of the mechanism of these alterations revealed that the difference in strength of these contacts is attributed to the different conformational state of the nucleosomal core and is caused by the stretching of the nucleosomal DNA upon chromatin decondensation during its activation. Histone terminal domains may be involved in this process through posttranslational modifications affecting chromatin condensation. The described localization of the histone H2A C-terminal domain in the nucleosome by crosslinking demonstrates the ability of this methodology to determine the location of histone terminal domains and thereby elucidate their role in nucleosome function. The results of the described experiments suggest that chromatin decondensation may alter the nucleosomal DNA conformation and affect the histone-DNA contacts resulting in a structural transition that may play a role in rendering the nucleosome competent for transcription and/or replication

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Authors and Affiliations

  1. Department of Biological Chemistry School of Medicine, University of California, Davis, CA, 95616, USA

    Sergei I. Usachenko & E. Morton Bradbury

  2. Life Science Division, Los Alamos National Laboratory, MS M888 Los Alamos, New Mexico, 87545, USA

    E. Morton Bradbury

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  1. Sergei I. Usachenko
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  2. E. Morton Bradbury
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Editors and Affiliations

  1. Life Sciences Division, Los Alamos National Laboratory, Los Alamos, New Mexico, USA

    E. Morton Bradbury

  2. Agricultural Biotechnology Centre, Gödöllõ, Hungary

    Sándor Pongor

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Usachenko, S.I., Bradbury, E.M. (1999). Histone-DNA Contacts in Structure/Function Relationships of Nucleosomes as Revealed by Crosslinking. In: Bradbury, E.M., Pongor, S. (eds) Structural Biology and Functional Genomics. NATO Science Series, vol 71. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4631-9_11

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  • DOI: https://doi.org/10.1007/978-94-011-4631-9_11

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-0-7923-5782-7

  • Online ISBN: 978-94-011-4631-9

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