Abstract
Developments in biotechnology have become a force to alter agriculture, and now place sharp focus on the biology of the rhizosphere as the site where modified plants and modified microorganisms will interact. Rhizosphere research, traditionally general and qualitative for the most part, must now address rhizosphere events in autecological terms to examine the specific microorganism in its interactions with its microenvironment and with other specific microbes therein. Extreme complexities of the rhizosphere call for innovative methodology. Molecular probes appear to offer the best approach to deal with the rhizosphere microhabitat with adequate specificity and sensitivity. Immunofluorescence, the first method to apply a molecular probe, has been used for autecological study of complex natural environments, including rhizospheres, for more than twenty years. Further developments in immunofluorescence, and its use together with refined complementary techniques will contribute increasingly to rhizosphere research. New molecular genetic probes are now emerging and, when successfully developed for application to soil systems, will provide a new generation of methodologies for autecological investigations of the rhizosphere.
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© 1991 Springer Science+Business Media Dordrecht
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Schmidt, E.L. (1991). Methods for microbial autecology in the soil rhizosphere. In: Keister, D.L., Cregan, P.B. (eds) The Rhizosphere and Plant Growth. Beltsville Symposia in Agricultural Research, vol 14. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3336-4_9
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DOI: https://doi.org/10.1007/978-94-011-3336-4_9
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