Abstract
Living organisms do not just grow by synthesizing cellular components. As part of the necessary steps for existence, some components are degraded after synthesis. Even for bacteria in balanced, exponential growth some substances, under some conditions, are turned over. In other phases of growth turnover can be much more extensive, but it is still selective. This review covers studies with animals as a way to put the studies on microorganisms in perspective. The history, the mathematics, and experimental design of turnover experiments are reviewed. The important conclusion is that most of the proteins during balanced growth are very stable in bacteria, although ribosomal proteins are degraded under starvation conditions. Another generalization is that the process of wall enlargement in general is associated with obligatory turnover of the peptidoglycan.
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References
Alberts B, Bray D, Lewis J, Raff M, Roberts K & Watson JD (1989) Molecular Biology of the Cell. Garland Publishing, Inc, New York
Alton TH & Koch AL (1974) Unused protein synthetic capacity of Escherichia coli grown in phosphate-limited chemostats. J. Mol. Biol. 86: 1–9
Borsook H & Keighley GL (1935) The ‘zcontinuing’ metabolism of nitrogen in animals. Proc. Roy. Soc. London Ser. B. 118: 488–521
Coffman RL, Norris TE & Koch AL (1971) Chain elongation rate of messenger and polypeptides in slowly growing Escherichia coli. J. Mol. Biol. 60: 1–19
Darnell J, Lodish H & Baltimore D (1990) Molecular Cell Biogy. Scientific American Books, New York
Doyle RJ & Koch AL (1987) The functions of autolysins in the growth and division of Bacillus subtilis. Crit. Rev. Microbiol. 15: 169–222
Hall BG (1988) Adaptive evolution that requires multiple spon-taneous mutations. 1. Mutations involving an insertion sequence. Genetics 120: 887–897
Hall BG (1990) Spontaneous point mutations that occur more often when advantageous than when neutral. Genetics 126: 5–16
Hogness DS, Cohn M & Monod J (1955) Studies on the induced synthesis of β-galactosidase in Escherichia coli: the kinetics and mechanism of sulfur incorporation. Biochim. Biophys. Acta 16: 99–116
King J (1980) In: Goldberg RF (Ed) Biological Regulation andDevelopment, Vol 2 (pp 101–132). Plenum, New York
Koch AL (1962) The evaluation of the rates of biological processes from tracer kinetic data 1. The influence of labile metabolic pools. J. Theor. Biol. 3: 283–303
Koch AL (1968) The evaluation of the rates of biological processes from tracer kinetic data. II. RNA metabolism in growing bacteria. J. Theor. Biol. 18: 105–132
Koch AL (1971a) Evaluation of the rates of biological processes from tracer kinetic data. III. The net synthesis lemma and exchangeable pools. J. Theor. Biol. 32: 429–450
Koch AL (1971b) Evaluation of the biological processes from tracer kinetic data. IV. Digital simulation of nucleic acid metabolism in bacteria. J. Theor. Biol. 32: 451–469
Koch AL (1979) Microbial growth in low concentrations of nutrients. In: Shilo M (Ed) Strategies in Microbial Life in Extreme Environments (p 261–279). Dahlem Konferenzen-1978, Berlin
Koch AL, Higgins ML & Doyle RJ (1982) The role of surface stress in the morphology of microbes. J. Gen. Microbiol. 128: 927–945
Koch AL & Coffman R (1970) Diffusion, permeation, or enzyme limitation: A probe for the kinetics of enzyme induction. Biotech. and Bioeng. 12: 651–677
Koch AL & Deppe CS (1971) In vivo assay of protein synthesizing capacity of Escherichia coli from slowly growing chemostat cultures. J. Mol. Biol. 55: 549–562
Koch AL & Doyle RJ (1985) Inside-to-outside growth and the turnover of the Gram-positive rod. J. Theor. Biol. 117: 137–157
Koch AL & Levy FIR (1955) Protein turnover in growing cultures of Escherichia coli. J. Biol. Chem. 217: 947–957
Lutkenhaus JF, Moore BA, Masters M & Donachie WD (1979) Individual proteins are synthesized continuously inthroughout the Escherichia coli cell cycle. J. Bacteriol. 138: 352–360
Mandelstam, J (1958) Turnover of protein in growing and non-growing populations of Escherichia coli. Biochemical J. 69: 110–119
Mandelstam, J (1960) The intracellular turnover of protein and nucleic acid in its role in bacterial differentiation. Bacteriol. Rev. 24: 289–308
Matin A, Auger EA, Blum PH & Schultz JE (1989) Genetic starvation survival in nondiffcrentiating bacteria Ann. Rev. Microbiol. 43: 293–316
Monod J (1958) An outline of enzyme induction. Recueil Travaux Chim. Pays-bas. 7: 569–585
Nath K & Koch AL (1971) Protein degradation in Escherichia co/i. II. Strain differences in the degradation of protein and nucleic acid resulting from starvation. J. Biol. Chem. 246: 6956–6967
Pine MJ (1972) Turnover of intracellular proteins. Ann Rev. Microbiol. 26: 103–125
Rotman B & Spiegelman S (1954) On the origin of the carbon in induced synthesis of β-galactosidase in Escherichia coli. J. Bacteriol. 68: 419–429
Samarel AM (1991) In vivo measurement of protein turnover during muscle growth and atrophy. FASEB J. 5: 2020–2028
Schoenheimer R (1942) The dynamic state of body constiuents. Cambridge
Stragier P. Kunkel B, Kroos L & Losick R. (1989). Chromosomal rearrangement generating a composite gene fro a developmental transcription factor. Science 243: 507–512
Zak R, Martin AF & Blough R (1979) Assessment of protein turnover by use of radioisotopic tracers. Physiol. Rev. 59: 407–447
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© 1992 Springer Science+Business Media Dordrecht
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Koch, A.L. (1992). Quantitative aspects of cellular turnover. In: Stouthamer, A.H. (eds) Quantitative Aspects of Growth and Metabolism of Microorganisms. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2446-1_5
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DOI: https://doi.org/10.1007/978-94-011-2446-1_5
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