Abstract
A new glycol methacrylate (GMA) polar resin technique was used for light microscopy and histochemistry of Macrotrachela quadricornifera. Animals were treated with cold aqueous solution of ethylene glycol (EG), then embedded in the cold. Animals required no conventional chemical fixation as EG stabilizes, dehydrates and cryopreserves their structure. In this way several enzymatic activities are preserved, along with morphology. Moreover, resin reticulate protects tissues against agressive reagents. As a consequence, it is possible to perform different staining procedures, in sequence, on the same section.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Barrow, B. J., R. Ortiz-Reyes, M. A. O’Ryordan&T. P. Pretlow,1989. In situ localization of enzymes and mucin in normal rat colon embedded in plastic. Histochem. J. 21: 289–295.
Dore, B. & P. Usai, 1986. Glycol Methacrylate embedding for enzyme preservation. Bas. appl. histochem. 30 suppl.: 82.
Dore, B. & P. Usai, 1990. Rivelazione di più attività enzimatiche e colorazioni multiple sulla stessa sezione. Nuove possibili applicazioni delle sezione semifini in resina idrofìla. 53° Congresso UZI, Palermo 1990.
Dore, B., G. Pons & P. Usai, 1989. Low temperature embedding and preservation of enzymatic activities without chemical fixation. Bas. appl. histochem., 33 suppl: 40.
Dore, B., F. Carnemolla & P. Usai, 1991. Quick freezing in liquid nitrogen. Ethylene Glycole substitution and embedding in Glycol Methacrylate at low temperature. Preparation of light microscopy specimens avoiding chemical fixation.Bas. appl. histochem. 35 suppl: 37.
Dore, B., G. Pons & P. Usai, 1991. Polar resins embedding without chemical fixation. Immunocytochemistry, enzymatic reaction and staining, in sequence, on the same section.Bas. appl. histochem., 35 suppl: 37.
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 1993 Springer Science+Business Media Dordrecht
About this paper
Cite this paper
Dore, B., Carnemolla, F. (1993). Polar resin embedding for Macrotrachela quadricornifera . In: Gilbert, J.J., Lubzens, E., Miracle, M.R. (eds) Rotifer Symposium VI. Developments in Hydrobiology, vol 83. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-1606-0_7
Download citation
DOI: https://doi.org/10.1007/978-94-011-1606-0_7
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-4700-5
Online ISBN: 978-94-011-1606-0
eBook Packages: Springer Book Archive