Summary
Somatic embryogenesis in grapes has been described by several authors, but till now a regeneration procedure applicable to all major cultivars has not been found. Young leaves and petioles of 3 grapevine cultivars (Barbera, Dolcetto, Nebbiolo) and of tetraploid plants of the cv Barbera were cultured in vitro to obtain somatic embryogenesis. In addition, endosperm was taken from mature seeds of 2n and 4n Barbera. Leaf discs and petioles were also excised from plantlets grown in vitro. Basal media used were Murashige & Skoog (MS) and Nitsch & Nitsch (NN) with added BA and 2,4-D or NOA at different concentrations and combinations. Ovules collected 45 days after an thesis from 14 stenospermocarpic cultivars were cultured on NN medium added with IAA, GA3 and activated charcoal at different concentrations. Callus was easily obtained from all the explants with all the media and hormone combinations used. Root differentiation was observed on NN and MS media with added BA. Some ovules of stenospermocarpic cultivars Perlon, Superior S., Imperatrice, and Carina developed somatic embryos on the two media tested. Embryos were transferred to NN medium with BA; the plantlets that developed were transferred to NN without growth regulators.
contribution no. 276 of Centro Miglioramento Genetico Vite
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Abbreviations
- BA:
-
6-benzyladenine
- 2,4-D:
-
dichlorophenoxyacetic acid
- GA3 :
-
gibberellic acid
- IAA:
-
indolylacetic acid
- NOA:
-
2-naphthoxyacetic acid
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© 1994 Springer Science+Business Media Dordrecht
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Vallania, R. et al. (1994). Somatic embryogenesis in grapevine Vitis vinifera L.. In: Schmidt, H., Kellerhals, M. (eds) Progress in Temperate Fruit Breeding. Developments in Plant Breeding, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0467-8_84
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DOI: https://doi.org/10.1007/978-94-011-0467-8_84
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