Summary
In-ovule embryo culture was used for obtaining seedlings from immature peach embryos 1-4 mm in length. Rapid micropropagation of Redhaven cultivar and B 52/2 vineyard peach selection was achieved by shoot tip proliferation. The two-stage rooting system, with a short auxin exposure followed by transfer to growth regulator-free half-strength basal media, proved to work much better than any other system published so far. Cotyledon regeneration was combined with Agrobacterium mediated genetic transformation. A clear difference in fluorescence between negative controls and the regenerants obtained after cotyledon inoculation with A. tumefaciens carrying the GUS gene was detected after 24 h indicating the production of chimeric peach plants.
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Abbreviations
- BA:
-
benzyladenine
- Cb:
-
carbenicillin
- GA3 :
-
gibberellin
- GUS:
-
ß-glucuronidase
- IAA:
-
indole3- acetic acid
- IBA:
-
indole-3-butyric acid
- Kan:
-
kanamycin
- KIN:
-
kinetin
- NAA:
-
1-naphthaleneacetic acid
- TDZ:
-
N-phenyl-N'-1 ,2,3-thiadiazol-5-ylurea (Thidiazuron)
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© 1994 Springer Science+Business Media Dordrecht
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Ognjanov, V., Vujanić-Varga, D., Macet, K. (1994). Tissue culture approaches to peach improvement. In: Schmidt, H., Kellerhals, M. (eds) Progress in Temperate Fruit Breeding. Developments in Plant Breeding, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0467-8_78
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DOI: https://doi.org/10.1007/978-94-011-0467-8_78
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