Abstract
Here we propose novel and fundamental method toward realizing a single chromosomal DNA analysis for future human chromosomal sequencing. Long DNA sized over a few kilo-basepair, especially chromosomal DNA, exhibit random-coiled conformation in a solution. To obtain information on genome directly, stretched conformation is more suitable than random-coiled one. In this paper, we propose a novel method for stretching of chromosomal DNA in a miniaturized agarose gel applying a low frequency AC electric field.
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Reference
International Humane Genome Sequencing Consortium, Initial sequencing and analysis of the human genome, Nature, 409, 860–921 (2001).
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© 2001 Springer Science+Business Media New York
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Kaji, N., Ueda, M., Baba, Y. (2001). Novel Method for Stretching of Mega Base Pair DNA in a Microspace Toward a Single Chromosomal DNA Analysis. In: Ramsey, J.M., van den Berg, A. (eds) Micro Total Analysis Systems 2001. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-1015-3_34
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DOI: https://doi.org/10.1007/978-94-010-1015-3_34
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-3893-5
Online ISBN: 978-94-010-1015-3
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