Abstract
Procedures are given for both plant and animal tissues. They differ in the preparation of the lysate, after which the steps are the same for both plant and animal samples.
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References
Murray, M.G. and Thompson, W. F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acid Research 8: 4321–4325
Chirgwin, J. M. Przybyla, A.E. MacDonald, J., and Rutter, W.J. (1979). Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18: 5294–5299
Kirby, K.S. (1968). Isolation of nucleic acids with phenolic solvents. Methods in Enzymology. 12B: 87–98
Chomczynsiki, P. and Sacchi, N. (1987). Single step method for RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Annals of Biochemistry. 162: 156–159
Vogelstein, B. and Gillespie, D. (1979). Preparative and analytical purification of DNA from agarose. Proceedings of the National Academy of Sciences. USA. 76: 615–619
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© 1998 Chapman & Hall
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Gauch, S., Hermann, R., Feuser, P., Oelmüller, U., Bastian, H. (1998). Isolation of Total RNA Using Silica-Gel Based Membranes. In: Karp, A., Isaac, P.G., Ingram, D.S. (eds) Molecular Tools for Screening Biodiversity. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0019-6_16
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DOI: https://doi.org/10.1007/978-94-009-0019-6_16
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6496-5
Online ISBN: 978-94-009-0019-6
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