Abstract
The effects of selection on allele frequency at five genes with putative effect on shoot morphology were examined in two perennial ryegrass (Lolium perenne L.) populations undergoing recurrent selection. A synthetic C0 population was created by intercrossing five unrelated ryegrass genotypes within the EU FP5 project ‘GRASP’. Two rounds of selection (both positive and negative) for axillary tiller formation were performed, leading to selected populations C1 + and C1 −, respectively. The mean number of axillary tillers per plant was 2.18, 3.90 and 0.22 for C0, C1 + and C1 −, respectively. Five ryegrass genes putatively involved in the control of plant architecture and hormone response were SNP genotyped in all three populations. A test of selective neutrality (Waples’ test), which tests the hypothesis of genetic drift versus selection, was applied. This test indicated selection for the gene LpIAA1 in C1 −, where allele frequency changes could not be explained by genetic drift alone (p < 0.05). LpIAA1 belongs to a large family of genes, called Aux/IAA, which comprises genes that are auxin-regulated and were shown to control shoot morphology in Arabidopsis and rice.
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Acknowledgments
This study was funded by the EU framework V project GRASP (QLRT-2001-00862) and partly supported by the Fulbright Scholarship (Grantee ID 68433937).
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Brazauskas, G., Pašakinskienė, I., Lübberstedt, T. (2013). Estimation of Temporal Allele Frequency Changes in Ryegrass Populations Selected for Axillary Tiller Development. In: Barth, S., Milbourne, D. (eds) Breeding strategies for sustainable forage and turf grass improvement. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4555-1_10
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DOI: https://doi.org/10.1007/978-94-007-4555-1_10
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