Abstract
RNA interference (RNAi) refers to double-stranded RNA (dsRNA)-mediated gene silencing. Since its discovery, it has developed as a powerful tool in functional genomics, and to date it is widely used in insect genetic research. It is certain that the discovery of RNAi has augmented our understanding of ~20–30 nucleotide non-coding small RNA as critical regulators of gene expression and genome stability. Besides, gene silencing through RNAi has revolutionized the study of gene function, particularly in non-model and non-genome sequenced insect species, which is the case for most agricultural pest insects. Without doubt, it contains great potential for diverse applications in fundamental and applied research, for instance in gene therapy in medicine and disease control. More recent, a new hot point is to find a feasible way to use RNAi as an alternative method for practical application of crop protection to combat pest insects.
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References
Arimatsu Y, Kotanib E, Sugimurab Y, Furusawa T (2007) Molecular characterization of a cDNA encoding extracellular dsRNase and its expression in the silkworm, Bombyx mori. Insect Biochem Mol Biol 37:176–183
Auer C, Frederick R (2009) Crop improvement using small RNAs: applications and predictive ecological risk assessments. Trends Biotechnol 27:644–651
Baum JA, Bogaert T, Clinton W, Heck GR, Feldmann P, Ilagan O, Johnson S, Plaetinck G, Munyikawa T, Pleau M, Vaughn T, Roberts J (2007) Control of coleopteran insect pests through RNA interference. Nat Biotechnol 25:1322–1326
Bautista MAM, Miyata T, Miura K, Tanaka T (2009) RNA interference-mediated knockdown of a cytochrome P450, CYP6BG1, from the diamondback moth, Plutella xylostella, reduces larval resistance to permethrin. Insect Biochem Mol Biol 39:38–46
Bellés X (2010) Beyond Drosophila: RNAi in vivo and functional genomics in insects. Annu Rev Entomol 55:111–128
Caljon G, De Ridder K, De Baetselier P, Coosemans M, Van den Abbeele J (2010) Identification of a tsetse fly salivary protein with dual inhibitory action on human platelet aggregation. PLoS One 5:e9671
Cancino-Rodezno A, Alexander C, Villaseñor R, Pacheco S, Porta H, Pauchet Y, Soberón M, Gill SS, Bravo A (2010) The mitogen-activated protein kinase p38 is involved in insect defense against Cry toxins from Bacillus thuringiensis. Insect Biochem Mol Biol 40:58–63
Carthew RW, Sontheimer EJ (2009) Origins and mechanisms of miRNAs and siRNAs. Cell 136:642–655
Chen J, Tang B, Chen H, Yao Q, Huang X, Chen J, Zhang D, Zhang W (2010) Different functions of the insect soluble and membrane-bound trehalase genes in chitin biosynthesis revealed by RNA interference. PLoS One 5:e10133
Gantier MP, Williams BRG (2009) siRNA delivery not Toll-free. Nat Biotechnol 27:911–912
Gempe T, Hasselmann M, Schiøtt M, Hause G, Otte M, Beye M (2009) Sex determination in honeybees: two separate mechanisms induce and maintain the female pathway. PLoS Biol 7:e1000222
Ghanim M, Kontsedalov S, Czosneck H (2007) Tissue-specific gene silencing by RNA interference in the whitefly Bemisia tabaci (Gennadius). Insect Biochem Mol Biol 37:732–738
Gong LA, Yang XQ, Zhang BL, Zhong G, Hu M (2011) Silencing of Rieske iron-sulfur protein using chemically synthesised siRNA as a potential biopesticide against Plutella xylostella. Pest Manag Sci 67(5):514–520
Gordon KHJ, Waterhouse PM (2007) RNAi for insect-proof plants. Nat Biotechnol 25:1231–1232
Hail D, Hunter WB, Dowd SE, Bextine BR (2010) Expressed sequence tag (EST) survey of life stages of the potato psyllid, Bactericera cockerelli, using 454 pyrosequencing. Southwest Entomol 35(3):463–466
Hakim RS, Baldwin K, Smagghe G (2010) Regulation of midgut growth, development, and metamorphosis. Annu Rev Entomol 55:593–608
He Z-B, Cao Y-Q, Yin Y-P, Wang Z-K, Chen B, Peng G-X, Xia Y-X (2006) Role of hunchback in segment patterning of Locusta migratoria manilensis revealed by parental RNAi. Dev Growth Differ 48:439–445
Hughes CL, Kaufman TC (2000) RNAi analysis of deformed, proboscipedia and sex combs reduced in the milkweed bug Oncopeltus fasciatus: novel roles for Hox genes in the hemipteran head. Development 127:3683–3694
Hunter W, Ellis J, van Engelsdorp D, Hayes J, Westervelt D, Glick E, Williams M, Sela I, Maori E, Pettis J, Cox-Foster D, Paldi N (2010) Large-scale field application of RNAi technology reducing Israeli acute paralysis virus disease in honey bees (Apis mellifera, Hymenoptera: Apidae). PLoS Pathog 6:e1001160
Huvenne H, Smagghe G (2010) Mechanisms of dsRNA uptake in insects and potentials of RNAi for pest control: a review. J Insect Physiol 56:227–235
International Aphid Genomics Consortium (2010) Genome sequence of the pea aphid Acyrthosiphon pisum. PLoS Biol 8:e1000313
Jaubert-Possamai S, Le Trionnaire G, Bonhomme J, Christophides GK, Rispe C, Tagu D (2007) Gene knockdown by RNAi in the pea aphid Acyrthosiphon pisum. BMC Biotechnol 7:63
Karatolos N, Pauchet Y, Wilkinson P, Chauhan R, Denholm I, Gorman K, Nelson DR, Bass C, ffrench-Constant RH, Williamson MS (2011) Pyrosequencing the transcriptome of the greenhouse whitefly, Trialeurodes vaporariorum reveals multiple transcripts encoding insecticide targets and detoxifying enzymes. BMC Genomics 12:56. doi:10.1186/1471-2164-12-56
Kemp C, Imler J-L (2009) Antiviral immunity in Drosophila. Curr Opin Immunol 21:3–9
Kennedy S, Wang D, Ruvkun G (2004) A conserved siRNA-degrading RNase negatively regulates RNA interference in C. elegans. Nature 427:645–649
Khajuria C, Buschman LL, Chen M-S, Muthukrishnan S, Zhu KY (2010) A gut-specific chitinase gene essential for regulation of chitin content of peritrophic matrix and growth of Ostrinia nubilalis larvae. Insect Biochem Mol Biol 40:621–629
Khila A, Grbić M (2007) Gene silencing in the spider mite Tetranychus urticae: dsRNA and siRNA parental silencing of the Distal-less gene. Dev Genes Evol 217:241–251
Kurscheid S, Lew-Tabor AE, Valle MR, Bruyeres AG, Doogan VJ, Munderloh UG, Guerrero FD, Barrero RA, Bellgard MI (2009) Evidence of a tick RNAi pathway by comparative genomics and reverse genetics screen of targets with known loss-of-function phenotypes in Drosophila. BMC Mol Biol 10:26
Leshkowitz D, Gazit S, Reuveni E, Ghanim M, Czosnek H, McKenzie C, Shatters RL Jr, Brown JK (2006) Whitefly (Bemisia tabaci) genome project: analysis of sequenced clones from egg, instar, and adult (viruliferous and non-viruliferous) cDNA libraries. BMC Genomics 7:79
Liu S, Ding Z, Zhang C, Yang B, Liu Z (2010) Gene knockdown by intro-thoracic injection of double-stranded RNA in the brown planthopper, Nilaparvata lugens. Insect Biochem Mol Biol 40:666–671
Mao Y-B, Cai W-J, Wang J-W, Hong G-J, Tao X-Y, Wang L-J, Huang Y-P, Chen X-Y (2007) Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol. Nat Biotechnol 25:1307–1313
Mittapalli O, Bai XD, Mamidala P, Rajarapu SP, Bonello P, Herms DA (2010) Tissue-specific transcriptomics of the exotic invasive insect pest emerald ash borer (Agrilus planipennis). PLoS One 5(10):e13708. doi:10.1371/journal.pone.0013708
Moar WJ, Clark T, Segers G, Ramaseshadri P, Hibbard B, Head G (2010) dsRNA: the next generation of pyramided insect protection traits. Abstract book 58th annual meeting of the Entomological Society of America, 12–15 Dec 2010, San Diego, CA, p 108
Moazed D (2009) Small RNAs in transcriptional gene silencing and genome defence. Nature 457:413–420
Mutti NS, Park Y, Reese JC, Reeck GR (2006) RNAi knockdown of a salivary transcript leading to lethality in the pea aphid, Acyrthosiphon pisum. J Insect Sci 6(38):1–7
Mutti NS, Louis J, Pappan LK, Pappan K, Begum K, Chen M-S, Park Y, Dittmer N, Marshall J, Reese JC, Reeck GR (2008) A protein from the salivary glands of the pea aphid, Acyrthosiphon pisum, is essential in feeding on a host plant. Proc Natl Acad Sci U S A 105:9965–9969
Obbard DJ, Jiggins FM, Halligan DL, Little TJ (2006) Natural selection drives extremely rapid evolution in antiviral RNAi genes. Curr Biol 16:580–585
Price DRG, Gatehouse JA (2008) RNAi-mediated crop protection against insects. Trends Biotechnol 26:393–400
Robinson GE, Hackett KJ, Purcell-Miramontes M, Brown SJ, Evans JD, Goldsmith MR, Lawson D, Okamuro J, Robertson HM, Schneider DJ (2011) Creating a buzz about insect genomes. Science 331:1386
Rocha JJE, Korolchuk VI, Robinson IM, O’Kane CJ (2011) A phagocytic route for uptake of double-stranded RNA in RNAi. PLoS One 6(4):e19087. doi:10.1371/journal.pone.0019087
Rodríguez-Cabrera L, Trujillo-Bacallao D, Borrás-Hidalgo O, Wright DJ, Ayra-Pardo C (2010) RNAi-mediated knockdown of a Spodoptera frugiperda trypsin-like serine-protease gene reduces susceptibility to a Bacillus thuringiensis Cry1Ca1 protoxin. Environ Microbiol 12(11):2894–2903
Rogers DW, Baldini F, Battaglia F, Panico M, Dell A, Morris HR, Catteruccia F (2009) Transglutaminase-mediated semen coagulation controls sperm storage in the malaria mosquito. PLoS Biol 7:e1000272
Runo S, Alakonya A, Machuka J, Sinha N (2011) RNA interference as a resistance mechanism against crop parasites in Africa: a ‘Trojan horse’ approach. Pest Manag Sci 67(2):129–136
Sabin LR, Hanna SL, Cherry S (2010) Innate antiviral immunity in Drosophila. Curr Opin Immunol 22:4–9
Saleh MC, van Rij RP, Hekele A, Gillis A, Foley E, O’Farrell PH, Andino R (2006) The endocytic pathway mediates cell entry of dsRNA to induce RNAi silencing. Nat Cell Biol 8:793–802
Saleh MC, Tassetto M, van Rij RP, Goic B, Gausson V, Berry B, Jacquier C, Antoniewski C, Andino R (2009) Antiviral immunity in Drosophila requires systemic RNA interference spread. Nature 458:346–350
Shabalina SA, Koonin EV (2008) Origins and evolution of eukaryotic RNA interference. Trends Ecol Evol 23:578–587
Shakesby AJ, Wallace IS, Isaacs HV, Pritchard J, Roberts DM, Douglas AE (2009) A water-specific aquaporin involved in aphid osmoregulation. Insect Biochem Mol Biol 39:1–10
Sims D, Bursteinas B, Jain E, Gao QO, Baum B, Zvelebil M (2010) The FLIGHT Drosophila RNAi database 2010 update. Fly 4(4):344–348
Siomi H, Siomi MC (2009) On the road to reading the RNA-interference code. Nature 457:396–404
Swevers L, Liu J, Huvenne H, Smagghe G (2011) Search for limiting factors in the RNAi pathway in silkmoth tissues and the silkmoth-derived Bm5 cell line: the RNA-binding proteins R2D2 and Translin. PLoS One 6(5):e20250. doi:10.1371/journal.pone.0020250
Tan A, Palli SR (2008) Identification and characterization of nuclear receptors from the red flour beetle, Tribolium castaneum. Insect Biochem Mol Biol 38:430–439
Tang B, Wang S, Zhang F (2010) Two storage hexamerins from the beet armyworm Spodoptera exigua: cloning, characterization and the effect of gene silencing on survival. BMC Mol Biol 11:65
Terenius O, Papanicolaou A, Garbutt JS, Eleftherianos I, Huvenne H, Sriramana K, Albrechtsen M, An C, Aymeric J-L, Barthel A, Bebas P, Bitra K, Bravo A, Chevalier F, Collinge DP, Crava CM, de Maagd RA, Duvic B, Erlandson M, Faye I, Felföldi G, Fujiwara H, Futahashi R, Gandhe AS, Gatehouse HS, Gatehouse LN, Giebultowicz J, Gómez I, Grimmelikhuijzen CJ, Groot AT, Hauser F, Heckel DG, Hegedus DD, Hrycaj S, Huang L, Hull J, Iatrou K, Iga M, Kanost MR, Kotwica J, Li C, Li J, Liu J, Lundmark M, Matsumoto S, Meyering-Vos M, Millichap PJ, Monteiro A, Mrinal N, Niimi T, Nowara D, Ohnishi A, Oostra V, Ozaki K, Papakonstantinou M, Popadic A, Rajam MV, Saenko S, Simpson RM, Soberón M, Strand MR, Tomita S, Toprak U, Wang P, Wee CW, Whyard S, Zhang W, Nagaraju J, ffrench-Constant RH, Herrero S, Gordon K, Swevers L, Smagghe G (2011) RNA interference in Lepidoptera: an overview of successful and unsuccessful studies and implications for experimental design. J Insect Physiol 57:231–245
Tian H, Peng H, Yao Q, Chen H, Xie Q, Tang B, Zhang W (2009) Developmental control of a Lepidopteran pest Spodoptera exigua by ingestion of bacteria expressing dsRNA of a non-midgut gene. PLoS One 4:e6225
Tomari Y, Du T, Zamore PD (2007) Sorting of Drosophila small silencing RNAs. Cell 130:299–308
Tomoyasu Y, Miller SC, Tomita S, Schoppmeier M, Grossmann D, Bucher G (2008) Exploring systemic RNA interference in insects: a genome-wide survey for RNAi genes in Tribolium. Genome Biol 9:R10
Torres L, Almazán C, Ayllón N, Galindo RC, Rosario-Cruz R, Quiroz-Romero H, de la Fuente J (2011) Functional genomics of the horn fly, Haematobia irritans (Linnaeus, 1758). BMC Genomics 12:105. doi:10.1186/1471-2164-12-105
Turner CT, Davy MW, MacDiarmid RM, Plummer KM, Birch NP, Newcomb RD (2006) RNA interference in the light brown apple moth, Epiphyas postvittana (Walker) induced by double-stranded RNA feeding. Insect Mol Biol 15:383–391
Ulvila J, Parikka M, Kleino A, Sormunen R, Ezekowitz RA, Kocks C, Ramet M (2006) Double-stranded RNA is internalized by scavenger receptor-mediated endocytosis in Drosophila S2 cells. J Biol Chem 281:14370–14375
Vallier A, Vincent-Monégat C, Laurençon A, Heddi A (2009) RNAi in the cereal weevil Sitophilus spp Systemic gene knockdown in the bacteriome tissue. BMC Biotechnol 9:44
Wang XH, Aliyari R, Li WX, Li HW, Kim K, Carthew R, Atkinson P, Ding SW (2006) RNA interference directs innate immunity against viruses in adult Drosophila. Science 312:452–454
Wang XW, Luan JB, Li JM, Bao YY, Zhang CX, Liu SS (2010) De novo characterization of a whitefly transcriptome and analysis of its gene expression during development. BMC Genomics 11:400. doi:10.1186/1471-2164-11-400
Wang Y, Zhang H, Li H, Miao X (2011) Second-generation sequencing supply an effective way to screen RNAi targets in large scale for potential application in pest insect control. PLoS One 6(4):e18644. doi:10.1371/journal.pone.0018644
Wei Z, Yin Y, Zhang B, Wang Z, Peng G, Cao Y, Xia Y (2007) Cloning of a novel protease required for the molting of Locusta migratoria manilensis. Dev Growth Differ 49:611–621
Whyard S, Singh AD, Wong S (2009) Ingested double-stranded RNAs can act as species-specific insecticides. Insect Biochem Mol Biol 39:824–832
Winston WM, Molodowitch C, Hunter CP (2002) Systemic RNAi in C. elegans requires the putative transmembrane protein SID-1. Science 295:2456–2459
Xie Q, Guo H-S (2006) Systemic antiviral silencing in plants. Virus Res 118:1–6
Xue J, Bao Y-Y, B-l L, Cheng Y-B, Peng Z-Y, Liu H, Xu H-J, Zhu Z-R, Lou Y-G, Cheng J-A, Zhang C-X (2010) Transcriptome analysis of the brown planthopper Nilaparvata lugens. PLoS One 5(12):e14233. doi:10.1371/journal.pone.0014233
Yang Y, Zhu YC, Ottea J, Husseneder C, Leonard BR, Abel C, Huang F (2010) Molecular characterization and RNA interference of three midgut aminopeptidase N isozymes from Bacillus thuringiensis-susceptible and -resistant strains of sugarcane borer, Diatraea saccharalis. Insect Biochem Mol Biol 40:592–603
Zhang F, Guo H, Zheng H, Zhou T, Zhou Y, Wang S, Fang R, Qian W, Chen X (2010a) Massively parallel pyrosequencing-based transcriptome analyses of small brown planthopper (Laodelphax striatellus), a vector insect transmitting rice stripe virus (RSV). BMC Genomics 11:303
Zhang J, Liu X, Zhang J, Li D, Sun Y, Guo Y, Ma E, Zhu KY (2010b) Silencing of two alternative splicing-derived mRNA variants of chitin synthase 1 gene by RNAi is lethal to the oriental migratory locust, Locusta migratoria manilensis (Meyen). Insect Biochem Mol Biol 40:824–833
Zhang J, Zhang J, Yang M, Jia Q, Guo Y, Ma E, Zhu KY (2011) Genomics-based approaches to screening carboxylesterase-like genes potentially involved in malathion resistance in oriental migratory locust (Locusta migratoria manilensis). Pest Manag Sci 67:183–190
Zhou X, Qi FM, Scharf ME (2006) Social exploitation of hexamerin: RNAi reveals a major caste-regulatory factor in termites. Proc Natl Acad Sci U S A 103:4499–4504
Zhou X, Wheeler MM, Qi FM, Scharf ME (2008) RNA interference in the termite Reticulitermes flavipes through ingestion of double-stranded RNA. Insect Biochem Mol Biol 38:805–815
Zhu F, Parthasarathy R, Bai H, Woithe K, Kaussmann M, Nauen R, Harrison DA, Palli SR (2010) A brain-specific cytochrome P450 responsible for the majority of deltamethrin resistance in the QTC279 strain of Tribolium castaneum. Proc Natl Acad Sci U S A 107(19):8557–8562
Zhu F, Xu J, Palli R, Ferguson J, Palli SR (2011) Ingested RNA interference for managing the populations of the Colorado potato beetle, Leptinotarsa decemlineata. Pest Manag Sci 67(2):175–182
Acknowledgements
The authors acknowledge support for their research by the Fund for Scientific Research-Flanders (FWO-Vlaanderen), the Flemish agency for Innovation by Science and Technology (IWT-Vlaanderen), the Special Research Funds of Ghent University, in Belgium, and the General Secretariat for Research and Technology, Hellenic Republic Ministry of National Education and Religious Affairs, in Greece. Luc Swevers acknowledges the support with a scholarship for foreign researcher by the Special Research Fund of Ghent University.
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Swevers, L., Smagghe, G. (2012). Use of RNAi for Control of Insect Crop Pests. In: Smagghe, G., Diaz, I. (eds) Arthropod-Plant Interactions. Progress in Biological Control, vol 14. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-3873-7_7
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