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Single-Molecule Kinetic Analysis of Stochastic Signal Transduction Mediated by G-Protein Coupled Chemoattractant Receptors

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Cell Signaling Reactions

Abstract

Cellular chemotactic behaviors are typical examples of stochastic signal transduction in living cells that have been investigated in detail both experimentally and theoretically. In this chapter, we describe single-molecule kinetic analysis for stochastic signal transduction in chemotactic responses mediated by G protein-coupled chemoattractant receptors in order to give deeper understanding of the stochastic nature in chemotactic signaling processes. We also describe theoretical analysis of receptor-mediated chemotactic signaling, which reveals that noise generated in the transmembrane signaling by G protein-coupled chemoattractant receptors limits the precision of the gradient sensing. This suggests that receptor-G protein coupling and its modulation have an important role for improving the signal-to-noise ratio of chemotactic signals and thus cellular chemotaxis. Extending this beyond G protein signaling, combining single-molecule kinetic analysis with theoretical analysis offers a new tool in exploring the relationship between the kinetic properties of signaling molecules and their corresponding cellular responses in general.

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Acknowledgments

The authors thank all of the members of Stochastic Biocomputing Group in Osaka University for discussion and also thank Peter Karagiannis for critical reading of the manuscripts.

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Authors and Affiliations

  1. Laboratories for Nanobiology, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, 565-0871, Japan

    Yukihiro Miyanaga & Masahiro Ueda

  2. Japan Science and Technology Agency, Core Research for Evolutional Science and Technology, Suita, Osaka, 565-0871, Japan

    Yukihiro Miyanaga & Masahiro Ueda

Authors
  1. Yukihiro Miyanaga
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  2. Masahiro Ueda
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Correspondence to Yukihiro Miyanaga .

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Editors and Affiliations

  1. , Cellular Informatics Laboratory, RIKEN, Hirosawa 2-1, Wako, 351-0198, Japan

    Yasushi Sako

  2. Graduate School of Frontier Biosciences, Laboratories of Nanobiology, Osaka University, Yamadaoka 1-3, Suita, 565-871, Japan

    Masahiro Ueda

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Miyanaga, Y., Ueda, M. (2011). Single-Molecule Kinetic Analysis of Stochastic Signal Transduction Mediated by G-Protein Coupled Chemoattractant Receptors. In: Sako, Y., Ueda, M. (eds) Cell Signaling Reactions. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9864-1_2

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