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Problems Caused by Microbes and Treatment Strategies: Rapid Diagnostics of Microbiologically Influenced Corrosion (MIC) in Oilfield Systems with a DNA-Based Test Kit

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Applied Microbiology and Molecular Biology in Oilfield Systems

Abstract

In the past, many operators have encountered failures due to MIC in pipelines and topside facilities contaminated with sulphate-reducing bacteria (SRB). In some cases, severe pitting has resulted in flow lines being either abandoned or replaced (Davies and Scott, 2006). However, there are reports of little or no significant MIC in some systems, despite an apparent significant contamination with SRB (Maxwell, 2006). As most bacterial counts were conducted using serial dilution techniques such as the most probable number (MPN) technique selective enumeration of SRB strains (depending on the type of growth medium used) will inevitably be conducted. Therefore, high bacterial numbers derived from cultivation-based techniques do not necessarily correlate to high SRB numbers causing MIC in the production system (Larsen et al., 2005). In addition, MIC can be caused by other microbes such as sulphate-reducing archaea (SRA), methanogens and fermentative microbes (Larsen et al., 2008, 2009). Also most samples taken by the oil industry are water samples. However, the majority of microbial activity takes place in biofilms that attach to pipeline walls, well tubing and on the inside of topside facilities.

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References

  • Boopathy R, Daniels L (1991) Effect of pH on anaerobic mild steel corrosion by methanogenic bacteria. Appl Environ Microbiol 57:2104–2108

    CAS  Google Scholar 

  • Crolet J (2005) Microbial corrosion in the oil industry: a corrosionist’s view. In: Ollivier B, Magot M (eds) Petroleum microbiology. ASM Press, Washington, DC, pp 143–170

    Google Scholar 

  • Davies M, Scott PJB (2006) Oilfield water technology. (Houston, TX: NACE International, 2006), pp 213–242

    Google Scholar 

  • Gittel A, Sørensen K, Skovhus TL, Ingvorsen K, Schramm A (2009) Prokaryotic community structure and activity of sulfate reducers in production water from high-temperature oil reservoirs with and without nitrate treatment. Appl Environ Microbiol 75:7086–7096

    Article  CAS  Google Scholar 

  • Hansen LH, Larsen J, Jensen M, Thomsen US, Sørensen K, Lundgaard T, Skovhus TL (2009) The application of bioassays for evaluating in-situ biocide efficiency in offshore oil production in the North sea. SPE 121656, SPE International Symposium on Oilfield Chemistry 2009, The Woodlands, TX

    Google Scholar 

  • Hugenholtz P (2002) Exploring prokaryotic diversity in the genomic area. Genome Biol 3:1–8

    Article  Google Scholar 

  • Larsen J, Rasmussen K, Pedersen H, Sørensen K, Lundgaard T, Skovhus TL (2010) Consortia of MIC Bacteria and Archaea causing pitting corrosion in top side oil production facilities (Corrosion 2010, Paper 10252). (Houston, TX: NACE International, 2010)

    Google Scholar 

  • Larsen J, Skovhus TL, Agerbæk M, Thomsen TR, Nielsen PH (2006) Bacterial diversity study applying novel molecular methods on Halfdan produced waters (Corrosion 2006, Paper 06668). (Houston, TX: NACE International, 2006)

    Google Scholar 

  • Larsen J, Skovhus TL, Saunders AM, Højris B, Agerbæk M (2008) Molecular identification of MIC bacteria from scale and produced water: similarities and differences (Corrosion 2008, Paper 08652). (Houston, TX: NACE International, 2008)

    Google Scholar 

  • Larsen J, Sørensen K, Højris B, Skovhus TL (2009) Significance of troublesome sulfate-reducing prokaryotes (SRP) in oil field systems (Corrosion 2009, Paper 09389). (Houston, TX: NACE International, 2009)

    Google Scholar 

  • Larsen J, Zwolle S, Kjellerup BV, Frølund B, Nielsen JP, Nielsen PH (2005) Identification of bacteria causing souring and biocorrosion in the Halfdan field by application of new molecular techniques (Corrosion 2005, Paper 05629). (Houston, TX: NACE International, 2005)

    Google Scholar 

  • Magot M, Ollivier B, Patel BKC (2000) Microbiology of petroleum reservoirs. Antonie van Leeuwenhoek 77:103–116

    Article  CAS  Google Scholar 

  • Maxwell S (2006) Predicting microbially influenced corrosion (MIC) in seawater injection systems. SPE 100519, SPE International Oilfield Corrosion Symposium 2006, Aberdeen

    Google Scholar 

  • Roberge PR (1999) Microbes and biofouling. Handbook of corrosion engineering. McGraw-Hill, New York, p 195

    Google Scholar 

  • Skovhus TL, Højris B, Saunders AM, Thomsen TR, Agerbæk M, Larsen J (2009) Practical use of new microbiology tools in oil production. SPE Production Operations 24:180–186

    Google Scholar 

  • Woese CR, Kandler O, Wheelis ML (1990) Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya. Proc Natl Acad Sci 87:4576–4579

    Article  CAS  Google Scholar 

  • Zhu XY, Lubeck J, Kilbane JJ II (2003) Characterization of microbial communities in gas industry pipelines. Appl Environ Microbiol 69:5354–5363

    Article  CAS  Google Scholar 

Download references

Acknowledgements

Laboratory experiments and field monitoring were sponsored by DUC Partners (A.P. Møller-Mærsk, Shell and Chevron).

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Correspondence to Torben Lund Skovhus .

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Skovhus, T.L., Sørensen, K.B., Larsen, J. (2010). Problems Caused by Microbes and Treatment Strategies: Rapid Diagnostics of Microbiologically Influenced Corrosion (MIC) in Oilfield Systems with a DNA-Based Test Kit. In: Whitby, C., Skovhus, T. (eds) Applied Microbiology and Molecular Biology in Oilfield Systems. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9252-6_16

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