Abstract
Because defucosylation levels (deFuc%) of monoclonal antibodies (MAbs) must be regulated for quality control, it is quite important to analyze the factors that affect deFuc%. For the MAbs produced in the rat hybridoma cell line YB2/0, we found that osmolality of the culture medium is the major determinant of deFuc%. deFuc% was linearly correlated with osmolality, with r2 being as high as 0.92. In addition, deFuc% was not affected by the type of compound used for controlling osmolality (NaCl, KCl, fucose, fructose, creatine and mannitol). We succeeded in controlling deFuc% of MAbs by maintaining medium osmolality constant during cultures (for both perfusion and fed-batch cultures).
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References
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Acknowledgements
We are grateful to Drs. Mitsuo Sato, Kazuhisa Uchida, Mr. Hiroshi Takasugi, Mr. Kazutoshi Maki, and Mr. Noriyuki Takahashi for their helpful discussions and their technical assistance.
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Konno, Y. et al. (2010). Controlling Fucosylation Levels of Antibodies with Osmolality during Cell Culture. In: Kamihira, M., Katakura, Y., Ito, A. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3892-0_20
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DOI: https://doi.org/10.1007/978-90-481-3892-0_20
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