Abstract
Conventional cell-based assays commonly used in the drug discovery process are based on a monolayer (2-dimensional; 2D) cell culture system. The obtained drug efficacy data often has a poor correlation with the in vivo data, suggesting a need for a better in vitro model to mimic in vivo environment. NanoCulture Plate (NCP) is a novel experimental system developed for the 3-dimensional (3D) cell culture. The plate bottom has a special film covered with a nano-structure pattern which enables spheroid formation of various cell types. The spheroid formation on NCP depended on the NCP patterns, and medium types. At molecular level, the expression levels of proteins, HIF-1, VEGF and AKT that characterize the in vivo-like environment were altered in the spheroids on NCP compared to 2D cell culture. In addition, 3D spheroids showed different drug susceptibility depending on the combination of drugs and cell types compared to 2D cells. NCP is also useful for spheroid formation of normal cells such as hepatocytes, adipocytes, and mesenchymal stem cells. On NCP, the characteristics of cellular differentiation were maintained and emphasized compared to 2D cells. Hepatocytes secreted higher levels of albumin. Preadipocytes form 3D spheroids on the NCP, differentiate to adipocytes after the induction with the higher content of triglyceride. These results suggest that NCP can create an in vivo like environment as the spheroid. The NCP in vitro 3D culture system offers a new approach for more relevant cell based assays for drug screening and for cellular differentiation study to obtain in vivo comparable results.
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Ozawa, F. et al. (2010). A New Approach for Drug Discovery and Differentiation Study Using Cutting-Edge 3d Cell Culture System. In: Kamihira, M., Katakura, Y., Ito, A. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 16. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3892-0_16
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DOI: https://doi.org/10.1007/978-90-481-3892-0_16
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