Abstract
The cationic polymer polyethylenimine (PEI) is a well-known non-viral delivery vector for cell transfection. In particular, the latest generation based on the linear PEI is more efficient than the first generation of branched polymers to deliver genes into mammalian cells. An overview of the properties of PEI for gene delivery will be presented here.
Transient gene expression in mammalian cells is becoming an attractive alternative for recombinant protein production. Recently, fully chemically-defined media supporting the growth of non-adherent cells have been developed and are compatible with transfection based on non-viral delivery reagents. Both PEI and a novel polymer, named Fecturin™, are synthetic reagents free of animal components, able to transfect mammalian cells types (CHO, HEK-293, HEK-EBNA, NSO, …) which are widely used for production of recombinant protein in these new media and with high protein yields.
The great potential of cationic polymers to deliver plasmids expressing transgenes into mammalian cells and their advantages for biomanufacturing of recombinant proteins using transient gene expression is presented in this article.
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Biri, S., Stock, F., Adib, A., Erbacher, P. (2010). Delivery of Biomolecules with Non-Viral Vectors. In: Noll, T. (eds) Cells and Culture. ESACT Proceedings, vol 4. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-3419-9_19
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DOI: https://doi.org/10.1007/978-90-481-3419-9_19
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