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Involvement of MicroRNAs in Human Cancer: Discovery and Expression Profiling

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An Omics Perspective on Cancer Research

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Abstract

The initial discovery of the involvement of two microRNAs, miR-15a and miR-16-1, in human CLL opened the way to the myriad of studies that have now conclusively proved the central role of microRNAs in all human cancers. Gene expression studies revealed that hundreds of microRNAs are deregulated in cancer cells and functional studies clarified that microRNAs are involved in all the molecular and biological processes that drive tumorigenesis. These findings have greatly improved our understanding on the molecular basis of cancer and, even more importantly, laid the foundation for the exploitation of microRNAs in cancer therapy.

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References

  • Adams BD, Furneaux H, White BA (2007) The micro-ribonucleic acid (miRNA) miR-206 targets the human estrogen receptor-alpha (ERalpha) and represses ERalpha messenger RNA and protein expression in breast cancer cell lines. Mol Endocrinol 21:1132–1147

    Article  CAS  PubMed  Google Scholar 

  • Ahituv N, Zhu Y, Visel A et al (2007) Deletion of ultraconserved elements yields viable mice. PLoS Biol 5:e234

    Article  PubMed  CAS  Google Scholar 

  • Akao Y, Nakagawa Y, Naoe T (2006) let-7 microRNA functions as a potential growth suppressor in human colon cancer cells. Biol Pharm Bull 29:903–906

    Article  CAS  PubMed  Google Scholar 

  • Asangani IA, Rasheed SA, Nikolova DA et al (2008) MicroRNA-21 (miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion, intravasation and metastasis in colorectal cancer. Oncogene 27:2128–2136

    Article  CAS  PubMed  Google Scholar 

  • Bandres E, Cubedo E, Agirre X et al (2006) Identification by real-time PCR of 13 mature microRNAs differentially expressed in colorectal cancer and non-tumoral tissues. Mol Cancer 5:29

    Article  CAS  PubMed  Google Scholar 

  • Baskerville S, Bartel DP (2005) Microarray profiling of microRNAs reveals frequent coexpression with neighboring miRNAs and host genes. RNA 11:241–247

    Article  CAS  PubMed  Google Scholar 

  • Bemis LT, Chen R, Amato CM et al (2008) MicroRNA-137 targets microphthalmia-associated transcription factor in melanoma cell lines. Cancer Res 68:1362–1368

    Article  CAS  PubMed  Google Scholar 

  • Benvenuti S, Comoglio PM (2007) The MET receptor tyrosine kinase in invasion and metastasis. J Cell Physiol 213:316–325

    Article  CAS  PubMed  Google Scholar 

  • Berezikov E, Guryev V, van de Belt J et al (2005) Phylogenetic shadowing and computational identification of human microRNA genes. Cell 120:21–24

    Article  CAS  PubMed  Google Scholar 

  • Bernards R, Brummelkamp TR, Beijersbergen RL (2006) shRNA libraries and their use in cancer genetics. Nat Methods 3:701–706

    Article  CAS  PubMed  Google Scholar 

  • Berx G, Raspe E, Christofori G et al (2007) Pre-EMTing metastasis? Recapitulation of morphogenetic processes in cancer. Clin Exp Metastasis 24:587–597

    Article  CAS  PubMed  Google Scholar 

  • Birchmeier C, Birchmeier W, Gherardi E et al (2003) Met, metastasis, motility and more. Nat Rev Mol Cell Biol 4:915–925

    Article  CAS  PubMed  Google Scholar 

  • Bommer GT, Gerin I, Feng Y et al (2007) p53-mediated activation of miRNA34 candidate tumor-suppressor genes. Curr Biol 17:1298–1307

    Article  CAS  PubMed  Google Scholar 

  • Bottoni A, Zatelli MC, Ferracin M et al (2007) Identification of differentially expressed microRNAs by microarray: a possible role for microRNA genes in pituitary adenomas. J Cell Physiol 210:370–377

    Article  CAS  PubMed  Google Scholar 

  • Brummelkamp TR, Bernards R, Agami R (2002) Stable suppression of tumorigenicity by virus-mediated RNA interference. Cancer Cell 2:243–247

    Article  CAS  PubMed  Google Scholar 

  • Budhu A, Jia HL, Forgues M et al (2008) Identification of metastasis-related microRNAs in hepatocellular carcinoma. Hepatology 47:897–907

    Article  CAS  PubMed  Google Scholar 

  • Bumcrot D, Manoharan M, Koteliansky V et al (2006) RNAi therapeutics: a potential new class of pharmaceutical drugs. Nat Chem Biol 2:711–719

    Article  CAS  PubMed  Google Scholar 

  • Burk U, Schubert J, Wellner U et al (2008) A reciprocal repression between ZEB1 and members of the miR-200 family promotes EMT and invasion in cancer cells. EMBO Rep 9:582–589

    Article  CAS  PubMed  Google Scholar 

  • Butz K, Ristriani T, Hengstermann A et al (2003) siRNA targeting of the viral E6 oncogene efficiently kills human papillomavirus-positive cancer cells. Oncogene 22:5938–5945

    Article  CAS  PubMed  Google Scholar 

  • Cai X, Hagedorn CH, Cullen BR (2004) Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs. RNA 10:1957–1966

    Article  CAS  PubMed  Google Scholar 

  • Calin GA, Dumitru CD, Shimizu M et al (2002) Frequent deletions and down-regulation of micro-NA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia. Proc Natl Acad Sci USA 99:15524–15529

    Article  CAS  PubMed  Google Scholar 

  • Calin GA, Liu CG, Sevignani C et al (2004a) MicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemias. Proc Natl Acad Sci USA 101:11755–11760

    Article  CAS  PubMed  Google Scholar 

  • Calin GA, Sevignani C, Dumitru CD et al (2004b) Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers. Proc Natl Acad Sci USA 101:2999–3004

    Article  CAS  PubMed  Google Scholar 

  • Calin GA, Ferracin M, Cimmino A et al (2005) A MicroRNA signature associated with prognosis and progression in chronic lymphocytic leukemia. N Engl J Med 353:1793–1801

    Article  CAS  PubMed  Google Scholar 

  • Calin GA, Croce CM (2006) Genomics of chronic lymphocytic leukemia microRNAs as new players with clinical significance. Semin Oncol 33:167–173

    Article  CAS  PubMed  Google Scholar 

  • Calin GA, Liu CG, Ferracin M et al (2007) Ultraconserved regions encoding ncRNAs are altered in human leukemias and carcinomas. Cancer Cell 12:215–229

    Article  CAS  PubMed  Google Scholar 

  • Calin GA, Cimmino A, Fabbri M et al (2008) miR-15a and miR-16-1 cluster functions in human leukemia. Proc Natl Acad Sci USA 105:5166–5171

    Article  CAS  PubMed  Google Scholar 

  • Camps C, Buffa FM, Colella S et al (2008) hsa-miR-210 is induced by hypoxia and is an independent prognostic factor in breast cancer. Clin Cancer Res 14:1340–1348

    Article  CAS  PubMed  Google Scholar 

  • Chan JA, Krichevsky AM, Kosik KS (2005) MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells. Cancer Res 65:6029–6033

    Article  CAS  PubMed  Google Scholar 

  • Chen CZ, Li L, Lodish HF et al (2004) MicroRNAs modulate hematopoietic lineage differentiation. Science 303:83–86

    Article  CAS  PubMed  Google Scholar 

  • Chen C, Ridzon DA, Broomer AJ et al (2005) Real-time quantification of micro­RNAs by stem-loop RT-PCR. Nucleic Acids Res 33, e179

    Article  CAS  PubMed  Google Scholar 

  • Ciafre SA, Galardi S, Mangiola A et al (2005) Extensive modulation of a set of microRNAs in primary glioblastoma. Biochem Biophys Res Commun 334:1351–1358

    Article  CAS  PubMed  Google Scholar 

  • Cimmino A, Calin GA, Fabbri M et al (2005) miR-15 and miR-16 induce apoptosis by targeting BCL2. Proc Natl Acad Sci USA 102:13944–13949

    Article  CAS  PubMed  Google Scholar 

  • Corney DC, Flesken-Nikitin A, Godwin AK et al (2007) MicroRNA-34b and microRNA-34c are targets of p53 and cooperate in control of cell proliferation and adhesion-independent growth. Cancer Res 67:8433–8438

    Article  CAS  PubMed  Google Scholar 

  • Corsten MF, Miranda R, Kasmieh R et al (2007) MicroRNA-21 knockdown disrupts glioma growth in vivo and displays synergistic cytotoxicity with neural precursor cell delivered S-TRAIL in human gliomas. Cancer Res 67:8994–9000

    Article  CAS  PubMed  Google Scholar 

  • Cummins JM, He Y, Leary RJ et al (2006) The colorectal microRNAome. Proc Natl Acad Sci USA 103:3687–3692

    Article  CAS  PubMed  Google Scholar 

  • Datta J, Kutay H, Nasser MW et al (2008) Methylation mediated silencing of MicroRNA-1 gene and its role in hepatocellular carcinogenesis. Cancer Res 68:5049–5058

    Article  CAS  PubMed  Google Scholar 

  • Dews M, Homayouni A, Yu D et al (2006) Augmentation of tumor angiogenesis by a Myc-activated microRNA cluster. Nat Genet 38:1060–1065

    Article  CAS  PubMed  Google Scholar 

  • Egle A, Harris AW, Bouillet P et al (2004) Bim is a suppressor of Myc-induced mouse B cell leukemia. Proc Natl Acad Sci USA 101:6164–6169

    Article  CAS  PubMed  Google Scholar 

  • Eis PS, Tam W, Sun L et al (2005) Accumulation of miR-155 and BIC RNA in human B cell lymphomas. Proc Natl Acad Sci USA 102:3627–3632

    Article  CAS  PubMed  Google Scholar 

  • Elmen J, Lindow M, Schutz S et al (2008a) LNA-mediated microRNA silencing in non-human primates. Nature 452:896–899

    Article  CAS  PubMed  Google Scholar 

  • Elmen J, Lindow M, Silahtaroglu A et al (2008b) Antagonism of microRNA-122 in mice by systemically administered LNA-antimiR leads to up-regulation of a large set of predicted target mRNAs in the liver. Nucleic Acids Res 36:1153–1162

    Article  CAS  PubMed  Google Scholar 

  • Esau C, Kang X, Peralta E et al (2004) MicroRNA-143 regulates adipocyte differentiation. J Biol Chem 279:52361–52365

    Article  CAS  PubMed  Google Scholar 

  • Feber A, Xi L, Luketich JD et al (2008) MicroRNA expression profiles of esophageal cancer. J Thorac Cardiovasc Surg 135:255–260

    Article  CAS  PubMed  Google Scholar 

  • Fire A, Xu S, Montgomery MK et al (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391:806–811

    Article  CAS  PubMed  Google Scholar 

  • Fluiter K, ten Asbroek AL, de Wissel MB et al (2003) In vivo tumor growth inhibition and biodistribution studies of locked nucleic acid (LNA) antisense oligonucleotides. Nucleic Acids Res 31:953–962

    Article  CAS  PubMed  Google Scholar 

  • Fluiter K, Frieden M, Vreijling J et al (2005) On the in vitro and in vivo properties of four locked nucleic acid nucleotides incorporated into an anti-H-Ras antisense oligonucleotide. Chembiochem 6:1104–1109

    Article  CAS  PubMed  Google Scholar 

  • Foekens JA, Sieuwerts AM, Smid M et al (2008) Four miRNAs associated with aggressiveness of lymph node-negative, estrogen receptor-positive human breast cancer. Proc Natl Acad Sci USA 105:13021–13026

    Article  CAS  PubMed  Google Scholar 

  • Fornari F, Gramantieri L, Ferracin M et al (2008) miR-221 controls CDKN1C/p57 and CDKN1B/p27 expression in human hepatocellular carcinoma. Oncogene 27:5651–5661

    Article  CAS  PubMed  Google Scholar 

  • Frankel LB, Christoffersen NR, Jacobsen A et al (2008) Programmed cell death 4 (PDCD4) is an important functional target of the microRNA miR-21 in breast cancer cells. J Biol Chem 283:1026–1033

    Article  CAS  PubMed  Google Scholar 

  • Gabriely G, Wurdinger T, Kesari S et al (2008) MicroRNA 21 promotes glioma invasion by targeting matrix metalloproteinase regulators. Mol Cell Biol 28:5369–5380

    Article  CAS  PubMed  Google Scholar 

  • Galardi S, Mercatelli N, Giorda E et al (2007) miR-221 and miR-222 expression affects the proliferation potential of human prostate carcinoma cell lines by targeting p27kip1. J Biol Chem 282:23716–23724

    Article  CAS  PubMed  Google Scholar 

  • Garzon R, Garofalo M, Martelli MP et al (2008a) Distinctive microRNA signature of acute myeloid leukemia bearing cytoplasmic mutated nucleophosmin. Proc Natl Acad Sci USA 105:3945–3950

    Article  CAS  PubMed  Google Scholar 

  • Garzon R, Volinia S, Liu CG et al (2008b) MicroRNA signatures associated with cytogenetics and prognosis in acute myeloid leukemia. Blood 111:3183–3189

    Article  CAS  PubMed  Google Scholar 

  • Gottardo F, Liu CG, Ferracin M et al (2007) Micro-RNA profiling in kidney and bladder cancers. Urol Oncol 25:387–392

    CAS  PubMed  Google Scholar 

  • Grady WM, Parkin RK, Mitchell PS et al (2008) Epigenetic silencing of the intronic microRNA hsa-miR-342 and its host gene EVL in colorectal cancer. Oncogene 27:3880–3888

    Article  CAS  PubMed  Google Scholar 

  • Gramantieri L, Ferracin M, Fornari F et al (2007) Cyclin G1 is a target of miR-122a, a microRNA frequently down-regulated in human hepatocellular carcinoma. Cancer Res 67:6092–6099

    Article  CAS  PubMed  Google Scholar 

  • Gregory PA, Bert AG, Paterson EL et al (2008) The miR-200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1. Nat Cell Biol 10:593–601

    Article  CAS  PubMed  Google Scholar 

  • Griffiths-Jones S, Grocock RJ, van Dongen S et al (2006) miRBase: microRNA sequences, targets and gene nomenclature. Nucleic Acids Res 34:D140–144

    Article  CAS  PubMed  Google Scholar 

  • Guimaraes-Sternberg C, Meerson A, Shaked I et al (2006) MicroRNA modulation of megakaryoblast fate involves cholinergic signaling. Leuk Res 30:583–595

    Article  CAS  PubMed  Google Scholar 

  • Guo Y, Chen Z, Zhang L et al (2008) Distinctive microRNA profiles relating to patient survival in esophageal squamous cell carcinoma. Cancer Res 68:26–33

    Article  CAS  PubMed  Google Scholar 

  • Han J, Lee Y, Yeom KH et al (2004) The Drosha-DGCR8 complex in primary microRNA processing. Genes Dev 18:3016–3027

    Article  CAS  PubMed  Google Scholar 

  • Han J, Lee Y, Yeom KH et al (2006) Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex. Cell 125:887–901

    Article  CAS  PubMed  Google Scholar 

  • Hanahan D, Weinberg RA (2000) The hallmarks of cancer. Cell 100:57–70

    Article  CAS  PubMed  Google Scholar 

  • Hayashita Y, Osada H, Tatematsu Y et al (2005) A polycistronic microRNA cluster, miR-17–92, is overexpressed in human lung cancers and enhances cell proliferation. Cancer Res 65:9628–9632

    Article  CAS  PubMed  Google Scholar 

  • He H, Jazdzewski K, Li W et al (2005a) The role of microRNA genes in papillary thyroid carcinoma. Proc Natl Acad Sci USA 102:19075–19080

    Article  CAS  PubMed  Google Scholar 

  • He L, Thomson JM, Hemann MT et al (2005b) A microRNA polycistron as a potential human oncogene. Nature 435:828–833

    Article  CAS  PubMed  Google Scholar 

  • Huang J, Wang F, Argyris E et al (2007) Cellular microRNAs contribute to HIV-1 latency in resting primary CD4+ T lymphocytes. Nat Med 13:1241–1247

    Article  CAS  PubMed  Google Scholar 

  • Huang Q, Gumireddy K, Schrier M et al (2008a) The microRNAs miR-373 and miR-520c promote tumor invasion and metastasis. Nat Cell Biol 10:202–210

    Article  CAS  PubMed  Google Scholar 

  • Huang YS, Dai Y, Yu XF et al (2008b) Microarray analysis of microRNA expression in hepatocellular carcinoma and non-tumorous tissues without viral hepatitis. J Gastroenterol Hepatol 23:87–94

    Article  PubMed  CAS  Google Scholar 

  • Ibarra I, Erlich Y, Muthuswamy SK et al (2007) A role for microRNAs in maintenance of mouse mammary epithelial progenitor cells. Genes Dev 21:3238–3243

    Article  CAS  PubMed  Google Scholar 

  • Iorio MV, Ferracin M, Liu CG et al (2005) MicroRNA gene expression deregulation in human breast cancer. Cancer Res 65:7065–7070

    Article  CAS  PubMed  Google Scholar 

  • Iorio MV, Visone R, Di Leva G et al (2007) MicroRNA signatures in human ovarian cancer. Cancer Res 67:8699–8707

    Article  CAS  PubMed  Google Scholar 

  • Isken F, Steffen B, Merk S et al (2008) Identification of acute myeloid leukemia associated microRNA expression patterns. Br J Haematol 140:153–161

    Article  CAS  PubMed  Google Scholar 

  • Jiang J, Gusev Y, Aderca I et al (2008) Association of MicroRNA expression in hepatocellular carcinomas with hepatitis infection, cirrhosis, and patient survival. Clin Cancer Res 14:419–427

    Article  CAS  PubMed  Google Scholar 

  • Johnson SM, Grosshans H, Shingara J et al (2005) RAS is regulated by the let-7 microRNA family. Cell 120:635–647

    Article  CAS  PubMed  Google Scholar 

  • Jopling CL, Yi M, Lancaster AM et al (2005) Modulation of hepatitis C virus RNA abundance by a liver-specific MicroRNA. Science 309:1577–1581

    Article  CAS  PubMed  Google Scholar 

  • Kefas B, Godlewski J, Comeau L et al (2008) microRNA-7 inhibits the epidermal growth factor receptor and the Akt pathway and is down-regulated in glioblastoma. Cancer Res 68:3566–3572

    Article  CAS  PubMed  Google Scholar 

  • Kim DH, Rossi JJ (2007) Strategies for silencing human disease using RNA interference. Nat Rev Genet 8:173–184

    Article  CAS  PubMed  Google Scholar 

  • Kim S, Lee UJ, Kim MN et al (2008) MicroRNA miR-199A* regulates the Met proto-oncogene and the downstream extracellular signal-regulated kinase 2 (ERK2). J Biol Chem 283:18158–18166

    Article  CAS  PubMed  Google Scholar 

  • Kim TY, Jong HS, Song SH et al (2003) Transcriptional silencing of the DLC-1 tumor suppressor gene by epigenetic mechanism in gastric cancer cells. Oncogene 22:3943–3951

    Article  CAS  PubMed  Google Scholar 

  • Kim VN, Nam JW (2006) Genomics of microRNA. Trends Genet 22:165–173

    Article  CAS  PubMed  Google Scholar 

  • Koralov SB, Muljo SA, Galler GR et al (2008) Dicer ablation affects antibody diversity and cell survival in the B lymphocyte lineage. Cell 132:860–874

    Article  CAS  PubMed  Google Scholar 

  • Korpal M, Lee ES, Hu G et al (2008) The miR-200 family inhibits epithelial-mesenchymal transition and cancer cell migration by direct targeting of E-cadherin transcriptional repressors ZEB1 and ZEB2. J Biol Chem 283:14910–14914

    Article  CAS  PubMed  Google Scholar 

  • Krutzfeldt J, Rajewsky N, Braich R et al (2005) Silencing of microRNAs in vivo with ‘antagomirs’. Nature 438:685–689

    Article  PubMed  CAS  Google Scholar 

  • Krutzfeldt J, Kuwajima S, Braich R et al (2007) Specificity, duplex degradation and subcellular localization of antagomirs. Nucleic Acids Res 35:2885–2892

    Article  CAS  PubMed  Google Scholar 

  • Kutay H, Bai S, Datta J et al (2006) Downregulation of miR-122 in the rodent and human hepatocellular carcinomas. J Cell Biochem 99:671–678

    Article  CAS  PubMed  Google Scholar 

  • Ladeiro Y, Couchy G, Balabaud C et al (2008) MicroRNA profiling in hepatocellular tumors is associated with clinical features and oncogene/tumor suppressor gene mutations. Hepatology 47:1955–1963

    Article  CAS  PubMed  Google Scholar 

  • Lagos-Quintana M, Rauhut R, Lendeckel W et al (2001) Identification of novel genes coding for small expressed RNAs. Science 294:853–858

    Article  CAS  PubMed  Google Scholar 

  • Landen CN Jr, Chavez-Reyes A, Bucana C et al (2005) Therapeutic EphA2 gene targeting in vivo using neutral liposomal small interfering RNA delivery. Cancer Res 65:6910–6918

    Article  CAS  PubMed  Google Scholar 

  • Lau NC, Lim LP, Weinstein EG et al (2001) An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans. Science 294:858–862

    Article  CAS  PubMed  Google Scholar 

  • Lawrie CH, Soneji S, Marafioti T et al (2007) MicroRNA expression distinguishes between germinal center B cell-like and activated B cell-like subtypes of diffuse large B cell lymphoma. Int J Cancer 121:1156–1161

    Article  CAS  PubMed  Google Scholar 

  • Lecellier CH, Dunoyer P, Arar K et al (2005) A cellular microRNA mediates antiviral defense in human cells. Science 308:557–560

    Article  CAS  PubMed  Google Scholar 

  • Lee DY, Deng Z, Wang CH et al (2007a) MicroRNA-378 promotes cell survival, tumor growth, and angiogenesis by targeting SuFu and Fus-1 expression. Proc Natl Acad Sci USA 104:20350–20355

    Article  CAS  PubMed  Google Scholar 

  • Lee EJ, Gusev Y, Jiang J et al (2007b) Expression profiling identifies microRNA signature in pancreatic cancer. Int J Cancer 120:1046–1054

    Article  CAS  PubMed  Google Scholar 

  • Lee RC, Feinbaum RL, Ambros V (1993) The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell 75:843–854

    Article  CAS  PubMed  Google Scholar 

  • Lee RC, Ambros V (2001) An extensive class of small RNAs in Caenorhabditis elegans. Science 294:862–864

    Article  CAS  PubMed  Google Scholar 

  • Lee Y, Ahn C, Han J et al (2003) The nuclear RNase III Drosha initiates microRNA processing. Nature 425:415–419

    Article  CAS  PubMed  Google Scholar 

  • Lee Y, Kim M, Han J et al (2004) MicroRNA genes are transcribed by RNA polymerase II. EMBO J 23:4051–4060

    Article  CAS  PubMed  Google Scholar 

  • Lewis BP, Burge CB, Bartel DP (2005) Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell 120:15–20

    Article  CAS  PubMed  Google Scholar 

  • Lin SL, Chiang A, Chang D et al (2008) Loss of miR-146a function in hormone-refractory prostate cancer. RNA 14:417–424

    Article  CAS  PubMed  Google Scholar 

  • Liu CG, Calin GA, Meloon B et al (2004) Anoligonucleotide microchip for genome-wide microRNA profiling in human and mouse tissues. Proc Natl Acad Sci U S A 101:9740–9744

    Article  CAS  PubMed  Google Scholar 

  • Lu J, Getz G, Miska EA et al (2005) MicroRNA expression profiles classify human cancers. Nature 435:834–838

    Article  CAS  PubMed  Google Scholar 

  • Ma L, Teruya-Feldstein J, Weinberg RA (2007) Tumor invasion and metastasis initiated by microRNA-10b in breast cancer. Nature 449:682–688

    Article  CAS  PubMed  Google Scholar 

  • Marcucci G, Radmacher MD, Maharry K et al (2008) MicroRNA expression in cytogenetically normal acute myeloid leukemia. N Engl J Med 358:1919–1928

    Article  CAS  PubMed  Google Scholar 

  • Mayr C, Hemann MT, Bartel DP (2007) Disrupting the pairing between let-7 and Hmga2 enhances oncogenic transformation. Science 315:1576–1579

    Article  CAS  PubMed  Google Scholar 

  • McManus MT, Sharp PA (2002) Gene silencing in mammals by small interfering RNAs. Nat Rev Genet 3:737–747

    Article  CAS  PubMed  Google Scholar 

  • Medina R, Zaidi SK, Liu CG et al (2008) MicroRNAs 221 and 222 bypass quiescence and compromise cell survival. Cancer Res 68:2773–2780

    Article  CAS  PubMed  Google Scholar 

  • Mendell JT (2008) miRiad roles for the miR-17–92 cluster in development and disease. Cell 133:217–222

    Article  CAS  PubMed  Google Scholar 

  • Meng F, Henson R, Lang M et al (2006) Involvement of human micro-RNA in growth and response to chemotherapy in human cholangiocarcinoma cell lines. Gastroenterology 130:2113–2129

    Article  CAS  PubMed  Google Scholar 

  • Meng F, Henson R, Wehbe-Janek H et al (2007) MicroRNA-21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer. Gastroenterology 133:647–658

    Article  CAS  PubMed  Google Scholar 

  • Mertens-Talcott SU, Chintharlapalli S, Li X et al (2007) The oncogenic microRNA-27a targets genes that regulate specificity protein transcription factors and the G2-M checkpoint in MDA-MB-231 breast cancer cells. Cancer Res 67:11001–11011

    Article  CAS  PubMed  Google Scholar 

  • Michael MZ, O’Connor SM, van Holst Pellekaan NG et al (2003) Reduced accumulation of specific microRNAs in colorectal neoplasia. Mol Cancer Res 1:882–891

    CAS  PubMed  Google Scholar 

  • Miller TE, Ghoshal K, Ramaswamy B et al (2008) MicroRNA-221/222 confers tamoxifen resistance in breast cancer by targeting p27(Kip1). J Biol Chem 283:29897–29903

    Article  CAS  PubMed  Google Scholar 

  • Murakami Y, Yasuda T, Saigo K et al (2006) Comprehensive analysis of microRNA expression patterns in hepatocellular carcinoma and non-tumorous tissues. Oncogene 25:2537–2545

    Article  CAS  PubMed  Google Scholar 

  • Naguibneva I, Ameyar-Zazoua M, Polesskaya A et al (2006) The microRNA miR-181 targets the homeobox protein Hox-A11 during mammalian myoblast differentiation. Nat Cell Biol 8:278–284

    Article  CAS  PubMed  Google Scholar 

  • Nam EJ, Yoon H, Kim SW et al (2008) MicroRNA expression profiles in serous ovarian carcinoma. Clin Cancer Res 14:2690–2695

    Article  CAS  PubMed  Google Scholar 

  • O’Donnell KA, Wentzel EA, Zeller KI et al (2005) c-Myc-regulated microRNAs modulate E2F1 expression. Nature 435:839–843

    Article  PubMed  CAS  Google Scholar 

  • Ota A, Tagawa H, Karnan S et al (2004) Identification and characterization of a novel gene, C13orf25, as a target for 13q31–q32 amplification in malignant lymphoma. Cancer Res 64:3087–3095

    Article  CAS  PubMed  Google Scholar 

  • Ozen M, Creighton CJ, Ozdemir M et al (2008) Widespread deregulation of microRNA expression in human prostate cancer. Oncogene 27:1788–1793

    Article  CAS  PubMed  Google Scholar 

  • Pallante P, Visone R, Ferracin M et al (2006) MicroRNA deregulation in human thyroid papillary carcinomas. Endocr Relat Cancer 13:497–508

    Article  CAS  PubMed  Google Scholar 

  • Pan Q, Luo X, Chegini N (2008) Differential expression of microRNAs in myometrium and leiomyomas and regulation by ovarian steroids. J Cell Mol Med 12:227–240

    Article  CAS  PubMed  Google Scholar 

  • Park SM, Gaur AB, Lengyel E et al (2008) The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2. Genes Dev 22:894–907

    Article  CAS  PubMed  Google Scholar 

  • Pasquinelli AE, Reinhart BJ, Slack F et al (2000) Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA. Nature 408:86–89

    Article  CAS  PubMed  Google Scholar 

  • Pekarsky Y, Santanam U, Cimmino A et al (2006) Tcl1 expression in chronic lymphocytic leukemia is regulated by miR-29 and miR-181. Cancer Res 66:11590–11593

    Article  CAS  PubMed  Google Scholar 

  • Perkins DO, Jeffries CD, Jarskog LF et al (2007) microRNA expression in the prefrontal cortex of individuals with schizophrenia and schizoaffective disorder. Genome Biol 8:R27

    Article  PubMed  CAS  Google Scholar 

  • Petrocca F, Visone R, Onelli MR et al (2008) E2F1-regulated microRNAs impair TGFbeta-dependent cell-cycle arrest and apoptosis in gastric cancer. Cancer Cell 13:272–286

    Article  CAS  PubMed  Google Scholar 

  • Porkka KP, Pfeiffer MJ, Waltering KK et al (2007) MicroRNA expression profiling in prostate cancer. Cancer Res 67:6130–6135

    Article  CAS  PubMed  Google Scholar 

  • Ramkissoon SH, Mainwaring LA, Ogasawara Y et al (2006) Hematopoietic-specific microRNA expression in human cells. Leuk Res 30:643–647

    Article  CAS  PubMed  Google Scholar 

  • Raymond CK, Roberts BS, Garrett-Engele P et al (2005) Simple, quantitative primer-extension PCR assay for direct monitoring of microRNAs and shortinterfering RNAs. RNA 11:1737–1744

    Article  CAS  PubMed  Google Scholar 

  • Raver-Shapira N, Marciano E, Meiri E et al (2007) Transcriptional activation of miR-34a contributes to p53-mediated apoptosis. Mol Cell 26:731–743

    Article  CAS  PubMed  Google Scholar 

  • Reinhart BJ, Slack FJ, Basson M et al (2000) The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. Nature 403:901–906

    Article  CAS  PubMed  Google Scholar 

  • Rodriguez A, Griffiths-Jones S, Ashurst JL et al (2004) Identification of mammalian microRNA host genes and transcription units. Genome Res 14:1902–1910

    Article  CAS  PubMed  Google Scholar 

  • Roldo C, Missiaglia E, Hagan JP et al (2006) MicroRNA expression abnormalities in pancreatic endocrine and acinar tumors are associated with distinctive pathologic features and clinical behavior. J Clin Oncol 24:4677–4684

    Article  CAS  PubMed  Google Scholar 

  • Ryan DG, Oliveira-Fernandes M, Lavker RM (2006) MicroRNAs of the mammalian eye display distinct and overlapping tissue specificity. Mol Vis 12:1175–1184

    CAS  PubMed  Google Scholar 

  • Sabbioni S, Callegari E, Spizzo R et al (2007) Anticancer activity of an adenoviral vector expressing short hairpin RNA against BK virus T-ag. Cancer Gene Ther 14:297–305

    Article  CAS  PubMed  Google Scholar 

  • Schetter AJ, Leung SY, Sohn JJ et al (2008) MicroRNA expression profiles associated with prognosis and therapeutic outcome in colon adenocarcinoma. JAMA 299:425–436

    Article  CAS  PubMed  Google Scholar 

  • Schmittgen TD, Jiang J, Liu Q et al (2004) A high-throughput method to monitor the expression of microRNA precursors. Nucleic Acids Res 32:e43

    Article  PubMed  CAS  Google Scholar 

  • Schulte JH, Horn S, Otto T et al (2008) MYCN regulates oncogenic MicroRNAs in neuroblastoma. Int J Cancer 122:699–704

    Article  CAS  PubMed  Google Scholar 

  • Schultz J, Lorenz P, Gross G et al (2008) MicroRNA let-7b targets important cell cycle molecules in malignant melanoma cells and interferes with anchorage-independent growth. Cell Res 18:549–557

    Article  CAS  PubMed  Google Scholar 

  • Scott GK, Goga A, Bhaumik D et al (2007) Coordinate suppression of ERBB2 and ERBB3 by enforced expression of micro-RNA miR-125a or miR-125b. J Biol Chem 282:1479–1486

    Article  CAS  PubMed  Google Scholar 

  • Sengupta S, den Boon JA, Chen IH et al (2008) MicroRNA 29c is down-regulated in nasopharyngeal carcinomas, up-regulating mRNAs encoding extracellular matrix proteins. Proc Natl Acad Sci USA 105:5874–5878

    Article  CAS  PubMed  Google Scholar 

  • Spaderna S, Schmalhofer O, Wahlbuhl M et al (2008) The transcriptional repressor ZEB1 promotes metastasis and loss of cell polarity in cancer. Cancer Res 68:537–544

    Article  CAS  PubMed  Google Scholar 

  • Spurgers KB, Gold DL, Coombes KR et al (2006) Identification of cell cycle regulatory genes as principal targets of p53-mediated transcriptional repression. J Biol Chem 281:25134–25142

    Article  CAS  PubMed  Google Scholar 

  • Subramanian S, Lui WO, Lee CH et al (2008) MicroRNA expression signature of human sarcomas. Oncogene 27:2015–2026

    Article  CAS  PubMed  Google Scholar 

  • Sylvestre Y, De Guire V, Querido E et al (2007) An E2F/miR-20a autoregulatory feedback loop. J Biol Chem 282:2135–2143

    Article  CAS  PubMed  Google Scholar 

  • Takamizawa J, Konishi H, Yanagisawa K et al (2004) Reduced expression of the let-7 microRNAs in human lung cancers in association with shortened postoperative survival. Cancer Res 64:3753–3756

    Article  CAS  PubMed  Google Scholar 

  • Tang G (2005) siRNA and miRNA: an insight into RISCs. Trends Biochem Sci 30:106–114

    Article  CAS  PubMed  Google Scholar 

  • Tanzer A, Stadler PF (2004) Molecular evolution of a microRNA cluster. J Mol Biol 339:327–335

    Article  CAS  PubMed  Google Scholar 

  • Tarasov V, Jung P, Verdoodt B et al (2007) Differential regulation of microRNAs by p53 revealed by massively parallel sequencing: miR-34a is a p53 target that induces apoptosis and G1-arrest. Cell Cycle 6:1586–1593

    Article  CAS  PubMed  Google Scholar 

  • Tavazoie SF, Alarcon C, Oskarsson T et al (2008) Endogenous human microRNAs that suppress breast cancer metastasis. Nature 451:147–152

    Article  CAS  PubMed  Google Scholar 

  • Tazawa H, Tsuchiya N, Izumiya M et al (2007) Tumor-suppressive miR-34a induces senescence-like growth arrest through modulation of the E2F pathway in human colon cancer cells. Proc Natl Acad Sci USA 104:15472–15477

    Article  CAS  PubMed  Google Scholar 

  • Tetzlaff MT, Liu A, Xu X et al (2007) Differential expression of miRNAs in papillary thyroid carcinoma compared to multinodular goiter using formalin fixed paraffin embedded tissues. Endocr Pathol 18:163–173

    Article  CAS  PubMed  Google Scholar 

  • Vasudevan S, Tong Y, Steitz JA (2007) Switching from repression to activation: microRNAs can up-regulate translation. Science 318:1931–1934

    Article  CAS  PubMed  Google Scholar 

  • Visone R, Pallante P, Vecchione A et al (2007) Specific microRNAs are downregulated in human thyroid anaplastic carcinomas. Oncogene 26:7590–7595

    Article  CAS  PubMed  Google Scholar 

  • Volinia S, Calin GA, Liu CG et al (2006) A microRNA expression signature of human solid tumors defines cancer gene targets. Proc Natl Acad Sci USA 103:2257–2261

    Article  CAS  PubMed  Google Scholar 

  • Voorhoeve PM, le Sage C, Schrier M et al (2006) A genetic screen implicates miRNA-372 and miRNA-373 as oncogenes in testicular germ cell tumors. Cell 124:1169–1181

    Article  CAS  PubMed  Google Scholar 

  • Vousden KH, Lane DP (2007) p53 in health and disease. Nat Rev Mol Cell Biol 8:275–283

    Article  CAS  PubMed  Google Scholar 

  • Wang T, Zhang X, Obijuru L et al (2007) A micro-RNA signature associated with race, tumor size, and target gene activity in human uterine leiomyomas. Genes Chromosomes Cancer 46:336–347

    Article  CAS  PubMed  Google Scholar 

  • Wang Y, Lee AT, Ma JZ et al (2008) Profiling microRNA expression in hepatocellular carcinoma reveals microRNA-224 up-regulation and apoptosis inhibitor-5 as a microRNA-224-specific target. J Biol Chem 283:13205–13215

    Article  CAS  PubMed  Google Scholar 

  • Weber F, Teresi RE, Broelsch CE et al (2006) A limited set of human microRNA is deregulated in follicular thyroid carcinoma. J Clin Endocrinol Metab 91:3584–3591

    Article  CAS  PubMed  Google Scholar 

  • Weiss GJ, Bemis LT, Nakajima E et al (2008) EGFR regulation by microRNA in lung cancer: correlation with clinical response and survival to gefitinib and EGFR expression in cell lines. Ann Oncol 19:1053–1059

    Article  CAS  PubMed  Google Scholar 

  • Welch C, Chen Y, Stallings RL (2007) MicroRNA-34a functions as a potential tumor suppressor by inducing apoptosis in neuroblastoma cells. Oncogene 26:5017–5022

    Article  CAS  PubMed  Google Scholar 

  • Wightman B, Ha I, Ruvkun G (1993) Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans. Cell 75:855–862

    Article  CAS  PubMed  Google Scholar 

  • Wilda M, Fuchs U, Wossmann W et al (2002) Killing of leukemic cells with a BCR/ABL fusion gene by RNA interference (RNAi). Oncogene 21:5716–5724

    Article  CAS  PubMed  Google Scholar 

  • Wirth T, Zender L, Schulte B et al (2003) A telomerase-dependent conditionally replicating adenovirus for selective treatment of cancer. Cancer Res 63:3181–3188

    CAS  PubMed  Google Scholar 

  • Wong QW, Lung RW, Law PT et al (2008a) MicroRNA-223 is commonly repressed in hepatocellular carcinoma and potentiates expression of Stathmin1. Gastroenterology 135:257–269

    Article  CAS  PubMed  Google Scholar 

  • Wong TS, Liu XB, Wong BY et al (2008b) Mature miR-184 as potential oncogenic microRNA of squamous cell carcinoma of tongue. Clin Cancer Res 14:2588–2592

    Article  CAS  PubMed  Google Scholar 

  • Xia L, Zhang D, Du R et al (2008) miR-15b and miR-16 modulate multidrug resistance by targeting BCL2 in human gastric cancer cells. Int J Cancer 123:372–379

    Article  CAS  PubMed  Google Scholar 

  • Xiao C, Srinivasan L, Calado DP et al (2008) Lymphoproliferative disease and autoimmunity in mice with increased miR-17–92 expression in lymphocytes. Nat Immunol 9:405–414

    Article  CAS  PubMed  Google Scholar 

  • Yanaihara N, Caplen N, Bowman E et al (2006) Unique microRNA molecular profiles in lung cancer diagnosis and prognosis. Cancer Cell 9:189–198

    Article  CAS  PubMed  Google Scholar 

  • Yang H, Kong W, He L et al (2008) MicroRNA expression profiling in human ovarian cancer: miR-214 induces cell survival and cisplatin resistance by targeting PTEN. Cancer Res 68:425–433

    Article  CAS  PubMed  Google Scholar 

  • Yoshinouchi M, Yamada T, Kizaki M et al (2003) In vitro and in vivo growth suppression of human papillomavirus 16-positive cervical cancer cells by E6 siRNA. Mol Ther 8:762–768

    Article  CAS  PubMed  Google Scholar 

  • Yu F, Yao H, Zhu P et al (2007) let-7 regulates self renewal and tumorigenicity of breast cancer cells. Cell 131:1109–1123

    Article  CAS  PubMed  Google Scholar 

  • Yu SL, Chen HY, Chang GC et al (2008) MicroRNA signature predicts survival and relapse in lung cancer. Cancer Cell 13:48–57

    Article  CAS  PubMed  Google Scholar 

  • Zhang L, Huang J, Yang N et al (2006a) microRNAs exhibit high frequency genomic alterations in human cancer. Proc Natl Acad Sci USA 103:9136–9141

    Article  CAS  PubMed  Google Scholar 

  • Zhang L, Volinia S, Bonome T et al (2008) Genomic and epigenetic alterations deregulate microRNA expression in human epithelial ovarian cancer. Proc Natl Acad Sci USA 105:7004–7009

    Google Scholar 

  • Zhang Z, Jiang G, Yang F et al (2006b) Knockdown of mutant K-ras expression by adenovirus-mediated siRNA inhibits the in vitro and in vivo growth of lung cancer cells. Cancer Biol Ther 5:1481–1486

    Article  CAS  PubMed  Google Scholar 

  • Zhu S, Si ML, Wu H et al (2007) MicroRNA-21 targets the tumor suppressor gene tropomyosin 1 (TPM1). J Biol Chem 282:14328–14336

    Article  CAS  PubMed  Google Scholar 

  • Zhu S, Wu H, Wu F et al (2008) MicroRNA-21 targets tumor suppressor genes in invasion and metastasis. Cell Res 18:350–359

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Dipartimento di Medicina Sperimentale e Diagnostica and Centro Interdipartimentale per la Ricerca sul Cancro, Università di Ferrara, Via Luigi Borsari, 46, 44100, Ferrara, Italy

    Massimo Negrini

  2. Department of Experimental Therapeutics, University of Texas, MD Anderson Cancer Center, Houston, TX, 77030, USA

    George A. Calin

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  1. Massimo Negrini
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Correspondence to Massimo Negrini .

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  1. Dept. Clinical Oncology, Queen Elizabeth Hospital, Gascoigne Road 30, Kowloon, Hong Kong/PR China

    William C.S. Cho

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Negrini, M., Calin, G.A. (2010). Involvement of MicroRNAs in Human Cancer: Discovery and Expression Profiling. In: Cho, W. (eds) An Omics Perspective on Cancer Research. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-2675-0_5

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