Abstract
Zanthoxylum gilletii is an indigenous tropical tree species that is valued for its structural timber, agroforestry and medicinal properties. Seed of many Zanthoxylum species have been reported to have poor germination. The study investigated the germination of fresh Z. gilletii seed harvested at two maturity stages, green and red (ripe) follicles, and harvested from two provenances: Kakamega and Koiwa in November and December 2006, respectively. Follicles were dried at controlled temperature of 20°C and relative humidity of 20%. During harvesting, Satinwood seed had high MC of 26%, which decreased to 12% after processing and drying. Seed dried to 12.5% MC were divided into two: one seed lot remained unwashed while the other was washed with sodium hydroxide (NaOH) solution. Unwashed seed from Kakamega and Koiwa provenances sown on sand in the glass house gave a germination of 3 and 8%, compared to seed washed with NAOH solution which germinated up to 10 and 23%, respectively, by the 17th week. Washed seed sown on 1% agar in incubators at various constant temperatures (20, 25, 30, and 35°C±1) and alternating temperatures (20/30°C and 15/35°C±1) recorded poor germination of less than 3%, probably due to the presence of chemicals which inhibited germination. These findings suggest that the hard seed coat and oil on the testa influence seed germination and possibly contribute to dormancy exhibited by Z. gilletii seed. Germination of seed from mature green and ripe fruits was similar and therefore Z. gilletii fruits should be harvested when they start to ripen. African Satinwood seed should be washed with soap solution to remove the oil film and fruit appendages on the testa before incubation. These results are still low and there is need for further investigation to improve germination of Z. gilletii seed.
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References
Adesina SK (2005) The Nigerian Zanthoxylum chemical and biological values. Afr J Tradit Complement Altern Med 2(3):282–301
Baskin CC, Baskin JM (1998) Seeds: ecology, biogeography, and evolution of dormancy and germination. Academic, San Diego, CA, 666pp
Baskin CC, Baskin JM (2004) A classification system for seed dormancy. Seed Sci Res 14:1–6
Beentje H, Adamson J (1994) The description of Rutaceae family: in Kenya trees, shrubs and lianas. 365–374
Bewley JD (1997) Seed germination and dormancy. Plant Cell 9:1055–1066
Bewley JD, Black M (1994) Seeds: physiology of development and germination. Plenum, New York, NY, 445pp
Bonner FT (1974) Zanthoxylum L., prickly-ash. In: Schopmeyer CS (ed) tech. cord. Seeds of woody plants in the USA. Agric. handbook. 450. USDA Forest Service, Washington, DC, pp 859–861
De Feo V, De Simone F, Senatore F (2002) Potential allelochemicals from the essential oil of Ruta graveolens. Phytochemistry 61(5):573–578
Finch-Savage and Metzger WE and Metzger GL (2006) Seed dormancy and the control of germination. New Physiol 171:501–523
Francis JK (1991) Zanthoxylum martinecence (Lam.) DC. espino rubial. SO-ITF-SM-42. USDA Forest Service, Southern Forest Experimental Station, New Orleans, LA, 5pp
GenStat (2003) GenStat for Windows (GenStat 7th Edition)
Gomez KA, Gomez AA (1984) Least significant differences and Duncan’s multiple range test. Statistical procedures for agricultural research. Wiley, New York, NY, pp 188–215
Hedberg I, Edwards S (eds) (1989) The description of Rutaceae family. Flora of Ethiopia, 3: Addis Ababa and Asmara Ethiopia Uppsala Sweden, pp 419–432
Hong TD, Ellis RH (1996) A protocol to determine seed storage behaviour. In: Engels JMM, Toll J (eds) Technical bulletin 2. International Plant Genetic Resources Institute, Rome, Italy
ISTA (2003) Handbook on seedling evaluation. 3rd edn. The International Seed Testing Association, Bassersdorf
ISTA (2004) International rules for seed testing. Seed Sci Technol 32(Suppl)
Kokwaro JO (1982) Flora of tropical East Africa, Rutaceae family 1–45. Prepared at the Royal Botanic Gardens/Kew with the assistance from the East African Herbarium. A.A. Balkema, Rotterdam
Microsoft Excel (2003) Microsoft Excel computer package
Munsell Colour Division (1972) Munsell colour charts for plant tissues, 2nd edn. Munsell Colour Division Kollmorgen Corporation, Baltimore
Njung׳e JT (1996) Species composition and regeneration at South Nandi Forest, Kenya. PhD thesis, University of Wales, Bangor, UK
Odhiambo KO, Njung׳e JT, Opondo-Mbai ML (2004) Some factors influencing regeneration of selected trees in Kakamega forest, Kenya. Discov Innovat 16(2/3):117–124
Omondi W, Maua JO, Gachathi FN (2004) Tree seed handbook of Kenya second edition. Kenya Forestry Research Institute (KEFRI), Nairobi, Kenya, pp 257–284
Owuor BO (1999) An ethnobotanical and phytochemical study of the herbal remedies of Migori District, Kenya. MSc thesis, University of Nairobi, Kenya
Pritchard HW, Sacandé M, Berjerk P (2004) Biological aspects of tropical tree seed desiccation and storage responses. In: Sacande M, Joker D, Duloo ME, Thomas KA (eds.) Comparative storage biology of tropical tree seed. IPGRI, Rome, Italy, pp 319–338
Rodriquez L (1995) Tratamientos pregerminativos para algunas espencies forestales nativas, de la Region Huetar Norte de Costa Rica. Simposio Latino Americano sobre semillas forestales. Managua, Nicaragua, 16–24 October, 1995
Sacande M, Joker D, Duloo ME, Thomas KA (2004) Comparative storage biology of tropical tree seeds. International Plant Genetic Resources Institute (IPGRI), Rome, Italy
Samarah NH, Allataifeh N, Turk MA, Tawaha AM (2004) Seed germination and dormancy of fresh and air-dried seed of common vetch (Vicia sativa L.) harvested at different stages of maturity. Seed Sci Technol 32:11–19
Sanon MD, Gamene CS, Sacande M, Neya O (2004) Desiccation and storage of Kigelia africana, Lophira lanceolata, Parinari curatellifolia and Zanthoxylum zanthoxyloides seeds from Burkina Faso. 16–23
Sautu A, Baskin JM, Baskin CC, Condit R (2006) Studies on the seed biology of 100 native species of trees in a moist tropical forest, Panama, Central America. Forest Ecol Manage 234:245–263
Setzer WN, Noletto JA, Lawton RO, Haber WA (2005) The leaf essential oil composition of Zanthoxylum species from Monteverde, Costa Rica. Mol Divers 9:3–13
Tigabu M, Fjellstrom J, Oden PC, Teketay D (2007) Germination of Juniperus procera seed in response to stratification and smoke treatments, and detection of insect-damaged seed with VIS + NIR spectroscopy. New Forests 33:155–169
Turner SR, Merritt DJ, Baskin CC, Dixon KW, Baskin JM (2005) Physical dormancy in seed of six genera of Australian Rhamnaceae. Seed Sci Res 15:51–58
World Agroforestry Centre (WAC) (2005) Zanthoxylum gilletii. From Agroforestry tree Database: a tree species reference and selection guide
Acknowledgements
This study was funded by Kenya Forestry Research Institute and Seed for Life Project and supervised by Moi University. I thank my supervisors Professor Reuben M. Muasya and Dr. Julius Ochuodho of Moi University and Mr. William O. Omondi of Kenya Forestry Research Institute. All those who assisted in seed collection, processing and monitoring experiments are acknowledged.
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Okeyo, M., Ochoudho, J., Muasya, R., Omondi, W. (2011). Investigation on the Germination of Zanthoxylum gilletii (African Satinwood) Seed. In: Bationo, A., Waswa, B., Okeyo, J., Maina, F., Kihara, J. (eds) Innovations as Key to the Green Revolution in Africa. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-2543-2_69
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